Comparison of Biochemical and Chemical Digestion and Detection Methods for Carbohydrates

Miloski, Katie; Wallace, K.; Fenger, Ashley; Schneider, E.; Bendinskas, Kestutis

doi:10.33697/ajur.2008.015

Cited by 22 publications

(18 citation statements)

References 8 publications

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“…For non-reducing sugars like sucrose, firstly, the acid was hydrolyzed, followed by incubating at 90 • C in a hot water bath for 15 min and then neutralized to pH 7 by NaOH 6N. Then, the reducing sugar detected by the DNS method [33].…”

Section: Detection Of Dry Cell Weight (Dcw) and Reducing Sugar In Thementioning

confidence: 99%

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

2020

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The coproduction of lipid and carotenoid by red yeasts in one cycle is more convenient and economical for the industrial sectors, while the kinetics correlation between both products under different culture conditions has been scarcely studied. This study is aiming to correlate the impact of different carbon sources, carbon to phosphorus ratio (C/P), temperature, aeration, pH, and metals on dry cell weight, lipid (GC and fluorescence microscope), and carotenoid (HPLC) production by Rhodotorula glutinis, and applying a novel feeding approach using a 5 L bioreactor to enhance carotenoid and unsaturated fatty acid production by R. glutinis. Whatever the culture condition is, the reversible correlation between lipid and carotenoid production was detected. Remarkably, when adding 0.1 mM BaCl2, cellular lipid was significantly increased 14% more than the control, with 79.3% unsaturated fatty acid (46% C18:2 and C18:3) and 50% γ-carotene, while adding 1 mM NiSO4, cellular carotenoid was enhanced around 53% than the control (torulene 88%) with 81% unsaturated fatty acid (61% oleic acid). Excitingly, 68.8 g/l biomass with 41% cellular lipid (79% unsaturated fatty acid) and 426 µgpigment/gdcw cellular carotenoid (29.3 mg/L) (71% torulene) were obtained, when the pH-temperature dual controlled process combined with metallo-sulfo-phospho-glucose feeding approach in the 5 L bioreactor during the accumulation phase was conducted. This is the first study on the kinetic correlation between lipid and carotenoid under different C/P ratio and the dual effect of different metals like NiSO4 on lipid and carotenoid production by red oleaginous yeasts, which in turn significant for enhancing the coproduction of lipid and carotenoid by R. glutinis.

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Section: Detection Of Dry Cell Weight (Dcw) and Reducing Sugar In Thementioning

confidence: 99%

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

2020

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“…Conventional chemical and biochemical analyses conducted to investigate the fruit ripening are limited by factors such as processing time and destructive nature [3]. ese methods are often laborious and expensive and require access to laboratory facility.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Ripening Degree of Avocado by Electrical Impedance Spectroscopy and Support Vector Machine

Islam

Wahid

Dinh

2018

Journal of Food Quality

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Avocado, a climacteric fruit, exerts high rate of respiration and ethylene production and thereby subject to ripening during storage. erefore, its ripening is a signi cant factor to impart optimum quality in postharvest storage. To understand the dynamics of ripening and to assess the degree of ripening in the avocado, electrical sensing technique is utilized in this study. In particular, electrical impedance spectroscopy (EIS) is found to uncover the physiological and structural characteristics in plants and vegetables and to follow physiological progressions due to environmental impacts. In this work, we present an approach that will integrate EIS and machine learning technique that allows us to monitor the ripening degree of the avocado. It is evident from our study that the impedance absolute magnitude of the avocado gradually decreases as the ripening stages ( rm, breaking, ripe, and overripe) proceed at a particular frequency. In addition, principal component analysis shows that impedance magnitude (two principal components combined explain 99.95% variation) has better discrimination capabilities for ripening degrees compared to impedance phase angle, impedance real part, and impedance imaginary part. Our classi er utilizes two principal component features over 100 EIS responses and demonstrates classi cation over rm, breaking, ripe, and overripe stages with an accuracy of 90%, precision of 93%, recall of 90%, f1-score of 90%, and auc of 88%. e study o ers plant scientists a low cost and nondestructive approach to monitor postharvest ripening process for quality control during storage.

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“…Total carbon (C), hydrogen (H), nitrogen (N), and sulfur (S) content in the sludge was measured using an elemental analyzer. While for the determination of phosphorus (P), standard reagent method using a spectrophotometer (DR 5000, HACH) at 420 nm was used (10).…”

Section: Proximate Analysis Of Sludgementioning

confidence: 99%

Electricity generation using membrane‐less microbial fuel cell powered by sludge supplemented with lignocellulosic waste

Makhtar

Tajarudin

2020

Int J Energy Res

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Summary A membrane‐less microbial fuel cell (ML‐MFC) is an electrochemical device that incorporates microorganisms into the design in order to produce electricity through biologically catalyzed oxidation of soluble, electron‐donating substrates. In this study, three lignocellulosic raw materials were added into the ML‐MFC whereby the sludge acted as the pseudomembrane. All three materials were used as the substrates in ML‐MFC for the production of electricity that was measured using a digital multimeter. Results showed that the ML‐MFC that contained sludge supplemented with banana peel produced the highest electricity, followed by corn bran and palm oil mill effluent (POME) at 237.1 mV (23.75 mW/m2), 176.8 mV (12.65 mW/m2), and 138 mV (22.03 mW/m2) after 138 h, 192 h, and 108 h of incubation period, respectively. For the control test (sludge only), about 162.7 mV was recorded at shorter incubation period (84 h). This showed that long‐term operation of the ML‐MFC using these complex lignocellulosic compounds as a direct substrate for electricity generation is feasible, though their degradation is slow.

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Comparison of Biochemical and Chemical Digestion and Detection Methods for Carbohydrates

Cited by 22 publications

References 8 publications

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

Assessment of Ripening Degree of Avocado by Electrical Impedance Spectroscopy and Support Vector Machine

Electricity generation using membrane‐less microbial fuel cell powered by sludge supplemented with lignocellulosic waste

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