Comparison of biochemical cartilage imaging techniques at 3 T MRI

Rehnitz, Christoph; Kupfer, J.; Streich, N.; Burkholder, Iris; Schmitt, Benjamin; Lauer, Lars; Kauczor, Hans‐Ulrich; Weber, Marc‐André

doi:10.1016/j.joca.2014.04.020

Cited by 58 publications

(97 citation statements)

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“…Here it was obvious that a severe OA could be detected with SE-sequences up to 94% and mild osteoarthritis could be discovered in 10% of the specimen [111]. In several other studies different MRI sequences were used to detect cartilage degeneration [15,116,117]. It was confirmed that even after MRI examinations with FLASH and DESS sequences low correlation in terms of sensitivity and specificity was found for both sequences in normal to mild Mankin grades [112].…”

Section: Discussionmentioning

confidence: 99%

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Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

et al. 2016

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IntroductionThe Göttingen Minipig (GM) is used as large animal model in articular cartilage research. The aim of the study was to introduce osteoarthritis (OA) in the GM by resecting the anterior cruciate ligament (ACLR) according to Pond and Nuki, verified by histological and magnetic resonance imaging (MRI) scoring as well as analysis of gene and protein expression.Materials and MethodsThe eight included skeletally mature female GM were assessed after ACLR in the left and a sham operation in the right knee, which served as control. 26 weeks after surgery the knee joints were scanned using a 3-Tesla high-field MR tomography unit with a 3 T CP Large Flex Coil. Standard proton-density weighted fat saturated sequences in coronal and sagittal direction with a slice thickness of 3 mm were used. The MRI scans were assessed by two radiologists according to a modified WORMS-score, the X-rays of the knee joints by two evaluators. Osteochondral plugs with a diameter of 4mm were taken for histological examination from either the main loading zone or the macroscopic most degenerated parts of the tibia plateau or condyle respectively. The histological sections were blinded and scored by three experts according to Little et al. Gene expression analysis was performed from surrounding cartilage. Expression of adamts4, adamts5, acan, col1A1, col2, il-1ß, mmp1, mmp3, mmp13, vegf was determined by qRT-PCR. Immunohistochemical staining (IH) of Col I and II was performed. IH was scored using a 4 point grading (0—no staining; 3-intense staining).Results and DiscussionSimilar signs of OA were evident both in ACLR and sham operated knee joints with the histological scoring result of the ACLR joints with 6.48 ± 5.67 points and the sham joints with 6.86 ± 5.84 points (p = 0.7953) The MRI scoring yielded 0.34 ± 0.89 points for the ACLR and 0.03 ± 0.17 for the sham knee joints. There was no correlation between the histological and MRI scores (r = 0.10021). The gene expression profiles as well as the immunohistochemical findings showed no significant differences between ACLR and sham knee joints. In conclusion, both knee joints showed histological signs of OA after 26 weeks irrespective of whether the ACL was resected or not. As MRI results did not match the histological findings, MRI was obviously unsuitable to diagnose the OA in GM. The analysis of the expression patterns of the 10 genes could not shed light on the question, whether sham operation also induced cartilage erosion or if the degeneration was spontaneous. The modified Pond-Nuki model may be used with reservation in the adult minipig to induce an isolated osteoarthritis.

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Section: Discussionmentioning

confidence: 99%

“…Development and grading of OA is mainly determined by histological analyses [4,6,14]. In humans, grade and prevalence of OA may be detected by MRI scanning [15,16] and through X-ray examination [17]. Thus, correlation of histological changes and MRI scanning is needed in diagnosis of OA.…”

Section: Introductionmentioning

confidence: 99%

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

et al. 2016

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“…To obtain dGEMRIC images, a 3D T 1 ‐weighted VIBE sequence with two excitation flip angles (5° and 26°), TR/TE 15/2.63 msec, voxel size 0.4 × 0.4 × 3 mm 3 , field of view (FOV) 140 × 140 mm 2 , imaging matrix 384 × 384, echo train length: 1 and total acquisition time of 5:24 minutes was performed based on similar protocols at the knee before (t = 0) and after (see timepoints below) intravenous administration of a double dose of gadopentetate dimeglumine (0.2 mmol/kg of Gd‐DTPA, Magnevist, Berlex Laboratories, Wayne, NJ). After Gd‐DTPA administration the subjects moved their wrist for 5 minutes in all directions, as exercise has been shown to increase contrast agent delivery to the joint .…”

Section: Methodsmentioning

confidence: 99%

Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) andT₂mapping at 3T MRI of the wrist: Feasibility and clinical application

Rehnitz

Klaan

Burkholder

et al. 2016

J. Magn. Reson. Imaging

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“…Shorter T2 values were found at higher field strengths [24]. In addition, the sequence type, coil architecture and calculation method of T2 mapping affect the T2 result [11].…”

Section: Discussionmentioning

confidence: 99%

MRI T2 Mapping of Knee Articular Cartilage Using Different Acquisition Sequences and Calculation Methods at 1.5 Tesla

Mars¹,

Chelli²,

Tbini³

et al. 2018

Med Princ Pract

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Objective: This study aims to determine how magnetic resonance imaging (MRI) acquisition techniques and calculation methods affect T2 values of knee cartilage at 1.5 tesla and to identify sequences that can be used for high-resolution T2 mapping in short scanning times. Materials and Methods: This study was performed on phantom and 29 patients who underwent MRI of the knee joint at 1.5 tesla. The protocol includes T2 mapping sequences based on Single-Echo Spin Echo (SESE), Multi-Echo Spin Echo (MESE), Fast Spin Echo (FSE) and Turbo Gradient Spin Echo (TGSE). The T2 relaxation times were quantified and evaluated using three calculation methods (MapIt, Syngo Offline and mono-exponential fit). signal-to-noise ratios (SNR) were measured in all sequences. All statistical analyses were performed using the t-test. Results: The average T2 values in phantom were 41.7 ± 13.8 ms for SESE, 43.2 ± 14.4 ms for MESE, 42.4 ± 14.1 ms for FSE and 44 ± 14.5 ms for TGSE. In the patient study, the mean differences were 6.5 ± 8.2 ms, 7.8 ± 7.6 ms and 8.4 ± 14.2 ms for MESE, FSE and TGSE compared to SESE, respectively; these statistical results were not significantly different (p > 0.05). The comparison between the three calculation methods showed no significant difference (p > 0.05). The t-test showed no significant difference between SNR values for all sequences. Conclusion: T2 values depend not only on the sequence type but also on the calculation method. None of the sequences revealed significant differences compared to the SESE reference sequence. TGSE with its short scanning time can be used for high-resolution T2 mapping.

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Comparison of biochemical cartilage imaging techniques at 3 T MRI

Abstract: CEST is able to detect normal and damaged cartilage and is non-inferior in distinguishing both when compared to dGEMRIC and T2 mapping.

Cited by 58 publications

References 56 publications

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) andT₂mapping at 3T MRI of the wrist: Feasibility and clinical application

MRI T2 Mapping of Knee Articular Cartilage Using Different Acquisition Sequences and Calculation Methods at 1.5 Tesla

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Comparison of biochemical cartilage imaging techniques at 3 T MRI

Abstract: CEST is able to detect normal and damaged cartilage and is non-inferior in distinguishing both when compared to dGEMRIC and T2 mapping.

Cited by 58 publications

References 56 publications

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) andT2mapping at 3T MRI of the wrist: Feasibility and clinical application

MRI T2 Mapping of Knee Articular Cartilage Using Different Acquisition Sequences and Calculation Methods at 1.5 Tesla

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Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) andT₂mapping at 3T MRI of the wrist: Feasibility and clinical application