“…The increment in DHA and TPH degradation might also be attributed to enhanced photosynthetic and metabolic activities of UV-exposed S. vacuolatus resulting from improved cellular activities after the UV exposure (Choi et al 2017 ; Ganapathy et al 2017 ; Kumar et al 2018 ). Previous studies by Megharaj et al ( 2000 ) and Eregie and Jamal ( 2019 , 2021 ) also reported an increase in DHA and TPH degradation of HCs using the Chlorella and Scenedesmus sp, respectively . Fig.…”
Section: Resultsmentioning
confidence: 77%
“…Scenedesmus vacuolatus isolated from spent lubricant oil waste (Eregie and Jamal 2019 ) was used for the UV mutagenesis in this study. Kultschar et al ( 2019 ) method was modified for the UV mutagenesis experiment.…”
Section: Methodsmentioning
confidence: 99%
“…The detailed BG11 growth medium constituent is described in section " Culture medium composition ". The culture flasks were incubated at 25 °C for 21 days and sampling were carried out at regular interval (Eregie and Jamal-Ally 2019 , 2021 ). Samples were thereafter analyzed for biomass concentration, cellular biomolecules (chlorophyll, carotenoid, protein, carbohydrate and lipid contents), growth rate, substrate versatility and substrate affinity.…”
This study presents the effect of ultra-violet (UV) light radiation on the process kinetics, metabolic performance, and biodegradation capability of Scenedesmus vacuolatus. The impact of the UV radiation on S. vacuolatus morphology, chlorophyll, carotenoid, carbohydrates, proteins, lipid accumulation, growth rate, substrate affinity and substrate versatility were evaluated. Thereafter, a preliminary biodegradative potential of UV-exposed S. vacuolatus on spent coolant waste (SCW) was carried out based on dehydrogenase activity (DHA) and total petroleum hydrocarbon degradation (TPH). Pronounced structural changes were observed in S. vacuolatus exposed to UV radiation for 24 h compared to the 2, 4, 6, 12 and 48 h UV exposure. Exposure of S. vacuolatus to UV radiation improved cellular chlorophyll (chla = 1.89-fold, chlb = 2.02-fold), carotenoid (1.24-fold), carbohydrates (4.62-fold), proteins (1.44-fold) and lipid accumulations (1.40-fold). In addition, the 24 h UV exposed S. vacuolatus showed a significant increase in substrate affinity (1/Ks) (0.959), specific growth rate (µ) (0.024 h−1) and biomass accumulation (0.513 g/L) by 1.50, 2 and 1.9-fold respectively. Moreover, enhanced DHA (55%) and TPH (100%) degradation efficiency were observed in UV-exposed S. vacuolatus. These findings provided major insights into the use of UV radiation to enhance S. vacuolatus biodegradative performance towards sustainable green environment negating the use of expensive chemicals and other unfriendly environmental practices.
Graphical abstract
“…The increment in DHA and TPH degradation might also be attributed to enhanced photosynthetic and metabolic activities of UV-exposed S. vacuolatus resulting from improved cellular activities after the UV exposure (Choi et al 2017 ; Ganapathy et al 2017 ; Kumar et al 2018 ). Previous studies by Megharaj et al ( 2000 ) and Eregie and Jamal ( 2019 , 2021 ) also reported an increase in DHA and TPH degradation of HCs using the Chlorella and Scenedesmus sp, respectively . Fig.…”
Section: Resultsmentioning
confidence: 77%
“…Scenedesmus vacuolatus isolated from spent lubricant oil waste (Eregie and Jamal 2019 ) was used for the UV mutagenesis in this study. Kultschar et al ( 2019 ) method was modified for the UV mutagenesis experiment.…”
Section: Methodsmentioning
confidence: 99%
“…The detailed BG11 growth medium constituent is described in section " Culture medium composition ". The culture flasks were incubated at 25 °C for 21 days and sampling were carried out at regular interval (Eregie and Jamal-Ally 2019 , 2021 ). Samples were thereafter analyzed for biomass concentration, cellular biomolecules (chlorophyll, carotenoid, protein, carbohydrate and lipid contents), growth rate, substrate versatility and substrate affinity.…”
This study presents the effect of ultra-violet (UV) light radiation on the process kinetics, metabolic performance, and biodegradation capability of Scenedesmus vacuolatus. The impact of the UV radiation on S. vacuolatus morphology, chlorophyll, carotenoid, carbohydrates, proteins, lipid accumulation, growth rate, substrate affinity and substrate versatility were evaluated. Thereafter, a preliminary biodegradative potential of UV-exposed S. vacuolatus on spent coolant waste (SCW) was carried out based on dehydrogenase activity (DHA) and total petroleum hydrocarbon degradation (TPH). Pronounced structural changes were observed in S. vacuolatus exposed to UV radiation for 24 h compared to the 2, 4, 6, 12 and 48 h UV exposure. Exposure of S. vacuolatus to UV radiation improved cellular chlorophyll (chla = 1.89-fold, chlb = 2.02-fold), carotenoid (1.24-fold), carbohydrates (4.62-fold), proteins (1.44-fold) and lipid accumulations (1.40-fold). In addition, the 24 h UV exposed S. vacuolatus showed a significant increase in substrate affinity (1/Ks) (0.959), specific growth rate (µ) (0.024 h−1) and biomass accumulation (0.513 g/L) by 1.50, 2 and 1.9-fold respectively. Moreover, enhanced DHA (55%) and TPH (100%) degradation efficiency were observed in UV-exposed S. vacuolatus. These findings provided major insights into the use of UV radiation to enhance S. vacuolatus biodegradative performance towards sustainable green environment negating the use of expensive chemicals and other unfriendly environmental practices.
Graphical abstract
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