Comparison of bovine pericardium decellularization protocols for production of biomaterial for cardiac surgery

Sokol, A. A.; Grekov, D. A.; Yemets, G. I.; Galkin, A. Yu.; Shchotkina, N.; Dovghaliuk, A. A.; Telehuzova, O. V.; Руденко, Н. М.; Romaniuk, O. M.; Yemets, I. M.

doi:10.7124/bc.000a3c

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…Post-decellularization, treatment with low concentrations of enzymes is used to wash away remnant nuclear waste from the matrix. Sokol et al 241 tested multiple standardized detergent-based pericardium decellularization protocols and found that while multiple detergent-based protocols adequately removed cellular content, protocols which use single step detergent or enzyme decellularization were most likely to negatively impact the collagen structure of the resulting matrix. Single step decellularization also led to higher levels of trace nucleic acid in the matrix than combinative approaches.…”

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

“…Single step decellularization also led to higher levels of trace nucleic acid in the matrix than combinative approaches. 241 SDS and Trypsin enzyme combinative approaches in particular are of interest, as they were shown to result in the most optimal proteomic and biomechanical results. 241 …”

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

“… 241 SDS and Trypsin enzyme combinative approaches in particular are of interest, as they were shown to result in the most optimal proteomic and biomechanical results. 241 …”

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

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Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

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Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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“…SDS is an effective decellularization agent and successfully applied in the decellularization of many tissues and whole organs [1][2][3]. However, many studies based on ECM ultrastructure report deleterious effects of SDS including reduction in collagen [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…The concept of extracellular matrix (ECM) isolation of cells from native tissues and genetic material in order to produce a natural scaffold is a main goal of the decellularization. Clinically, ECM of biological tissues has been used in manufacturing heart valve prostheses, small-diameter vascular grafts, and biological patches [1][2][3]. The scientific literature confirms the effective use of ionic detergent SDS for decellularization process.…”

Section: Introductionmentioning

confidence: 93%

Microarchitectonic of Decellularized Bovine Pericardium Matrix

Shchotkina¹,

Sokol²,

Yemets³

et al. 2021

Proceedings of the 7th World Congress on New Technologies

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The use of xenotissue for the needs of regenerative and cardiac medicine is a promising area of tissue engineering. The decellularization process provides complete purification of the elastin-collagen matrix of the bovine pericardium from cells and their components. The use of high concentrations of sodium dodecyl sulfate and glutaraldehyde can lead to a damage of the matrix architectonics. Therefore, the purpose of this study was to study the microarchitectonics of the decellularized matrix using a low concentration of sodium dodecyl sulfate (0.1% solution) and avoiding glutaraldehyde. Further stabilization and fixation of the matrix was carried out using 10 mM 1-Ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride and 10 mM N-Hydroxysuccinimide. The effect of decellularization was assessed by staining the samples with hematoxylin-eosin and by scanning electron microscopy. The research results confirmed the absence of structural changes in the collagen-elastin fibers of the matrix. Thus, the efficiency of using the proposed technology for obtaining a decellularized matrix based on the bovine pericardium was confirmed.

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