Comparison of Calcium and Silicate Cement and Mineral Trioxide Aggregate Biologic Effects and Bone Markers Expression in MG63 Cells

Chen, Chih-Lin; Huang, Tsui‐Hsien; Ding, Shinn-Jyh; Shie, Ming‐You; Kao, Chia‐Tze

doi:10.1016/j.joen.2009.02.002

Cited by 79 publications

(68 citation statements)

References 20 publications

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“…17e19 The increase in OPN expression by MTA and EMD was demonstrated to be time dependent in MG63 cells and murine cementoblasts, respectively. 20,21 Although the experimental period of this study was not long enough to confirm those articles, levels of OPN on Day 6 in both cells with MTA and EMD were higher than those on Day 3. Unlike OPN, few studies evaluated the expression of MMP-13 by MTA and EMD.…”

Section: Discussionmentioning

confidence: 56%

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Chung

Yang

Kim

et al. 2011

Journal of Dental Sciences

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KEYWORDS enamel matrix derivative; human cementumderived cells; matrix metalloproteinase-13; mineral trioxide aggregate Abstract Background/purpose: Cementogenesis is a critical event in the repair of endodontic perforations and the enamel matrix derivative (EMD) was reported to induce cementogenesis when used in periodontal disease. Yet, there is no study investigating the effects of EMD compared with mineral trioxide aggregate (MTA), a well-known material used for perforation repair. Because cementoblasts play important roles in the repair and regeneration of cementum, the aim of this study was to investigate the effect of EMD on the mineralizing potential of human cementum-derived cells (HCDCs). Materials and methods: HCDCs were cultured as previously described. ProRoot MTA was mixed and placed on the bottom of 6-well tissue culture plate inserts under aseptic conditions. These were allowed to set for 24 hours in a humidified incubator at 37 C. Cells were seeded onto culture plates at an initial density of 5000 cells/cm 2 and were treated with either MTA or EMD. Cells with only culture medium were used as the control. Levels of osteopontin and matrix metalloproteinase-13 were assessed by an enzyme-linked immunosorbent assay with supernatants of the culture media collected on Days 3 and 6 of incubation. Mineralization was detected on Day 6 by Alizarin red staining of cells. Results: Concentrations of osteopontin in the EMD group were significantly higher (P < 0.05) than those in the control group at all time points. On both Days 3 and 6, there was an increase in matrix metalloproteinase-13 expression in the EMD group compared with the control group, but the difference was significant only on Day 6 (P < 0.05).

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Section: Discussionmentioning

confidence: 56%

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Chung

Yang

Kim

et al. 2011

Journal of Dental Sciences

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“…10 Both the MTA-positive control group and the experimental laser treatment group under -100 kPa tension induced similar cellular reaction patterns. Morphologic evidence of PDL cell disruption at tension sites in tooth movement has also been described after only 5 min of tension loading, further suggesting the involvement of an inflammatory mechanism.…”

Section: Discussionmentioning

confidence: 86%

“…The extracts of mineral trioxide aggregate (MTA), which have osteoinduction ability, were used as a positive control group. 10 After treatment, cells were analyzed for their proliferation and expression of cellular proteins.…”

Section: Tension Incubator and Diode Laser On Pdl Cellsmentioning

confidence: 99%

Low-Level Laser Effects on Simulated Orthodontic Tension Side Periodontal Ligament Cells

Huang

Liu²,

Chen³

et al. 2013

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to analyze proliferation, inflammation, and osteogenic effects on periodontal ligament (PDL) cells after low-level laser therapy (LLLT) under simulated orthodontic tension conditions. Background data: Low-level lasers affect fibroblast proliferation and collagen synthesis and reduce inflammation. Few studies have focused on the LLLT changes in the PDL caused by moving teeth. Materials and methods: A human PDL cell line was cultured in a -100 kPa tension incubator. The PDL cells were treated with a 670 nm low-level diode laser, output power of 500 mW (continuous wave modus) for 2.5 or 5 sec, spot area 0.25 cm 2 , corresponding to 1.25 and 2.5 J at an energy density of 5 or 10 J/cm 2 , respectively. PDL cell viability was assayed by detecting the ability of the cells to cleave tetrazolium salt to formazan dye. Inflammation and osteogenic markers were analyzed by Western blot analysis. Results: PDL cell viablity increased in the experimental group, based on the ability of the cells to cleave tetrazolium salt at day 7 ( p < 0.05). The experimental group showed no difference in PDL cellular morphology compared with the control group. The inflammation markers inducible NO synthase (iNOS), cyclooxygenase (COX)-2 and interleukin (IL)-1 showed stronger expression in 5 and 10 J/cm 2 therapy at days 1 and 5, but decreased in expression at day 7. The osteogenic marker osteocalcin (OC) expression level was significantly higher at day 7 ( p < 0.05) than in the control cells. Conclusions: LLLT significantly increased PDL cell proliferation, decreased PDL cell inflammation, and increased PDL OC activity under the tension conditions used in this study.

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“…The CS cement was made according to our previously reported laboratory procedures (14). According to the MTA manufacturer's instructions, a liquid/powder ratio of 0.3 mL/g was used to mix the cement.…”

Section: Mta and Cs Materials Preparationmentioning

confidence: 99%

“…The biocompatibility of MTA has been assessed both in vitro and in vivo (10)(11)(12)(13). MTA and CS cements exhibit good biocompatibility with bone cells (MG63) and appeared to have osteoconduction effects on bone cells in our previous study (14).…”

mentioning

confidence: 92%

Expression of the Inflammatory Marker Cyclooxygenase-2 in Dental Pulp Cells Cultured with Mineral Trioxide Aggregate or Calcium Silicate Cements

Chen

Kao

Ding

et al. 2010

Journal of Endodontics

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Comparison of Calcium and Silicate Cement and Mineral Trioxide Aggregate Biologic Effects and Bone Markers Expression in MG63 Cells

Cited by 79 publications

References 20 publications

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Low-Level Laser Effects on Simulated Orthodontic Tension Side Periodontal Ligament Cells

Expression of the Inflammatory Marker Cyclooxygenase-2 in Dental Pulp Cells Cultured with Mineral Trioxide Aggregate or Calcium Silicate Cements

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