Comparison of the effects of enamel matrix derivative and mineral trioxide aggregate on the mineralization potential of human cementum-derived cells

Abstract

KEYWORDS enamel matrix derivative; human cementumderived cells; matrix metalloproteinase-13; mineral trioxide aggregate Abstract Background/purpose: Cementogenesis is a critical event in the repair of endodontic perforations and the enamel matrix derivative (EMD) was reported to induce cementogenesis when used in periodontal disease. Yet, there is no study investigating the effects of EMD compared with mineral trioxide aggregate (MTA), a well-known material used for perforation repair. Because cementoblasts play important roles in the repair and regeneration of cementum, the aim of this study was to investigate the effect of EMD on the mineralizing potential of human cementum-derived cells (HCDCs). Materials and methods: HCDCs were cultured as previously described. ProRoot MTA was mixed and placed on the bottom of 6-well tissue culture plate inserts under aseptic conditions. These were allowed to set for 24 hours in a humidified incubator at 37 C. Cells were seeded onto culture plates at an initial density of 5000 cells/cm 2 and were treated with either MTA or EMD. Cells with only culture medium were used as the control. Levels of osteopontin and matrix metalloproteinase-13 were assessed by an enzyme-linked immunosorbent assay with supernatants of the culture media collected on Days 3 and 6 of incubation. Mineralization was detected on Day 6 by Alizarin red staining of cells. Results: Concentrations of osteopontin in the EMD group were significantly higher (P < 0.05) than those in the control group at all time points. On both Days 3 and 6, there was an increase in matrix metalloproteinase-13 expression in the EMD group compared with the control group, but the difference was significant only on Day 6 (P < 0.05).

The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells

“…In our current study, OPN expression was found to be up-regulated in the MTA/BMP-2 and MTA/EMD groups on day 3, suggesting that the mineralization induced by combination of MTA and BMP-2 or EMD in MC3T3-E1 cells may promote OPN expression during an early stage. These findings are consistent with the results of a previous study that OPN is up-regulated in EMD-treated MTA groups on day 3 14. In our previous study, we found that MTA and EMD significantly stimulated the mineralization of human cementum-derived cells, which is consistent with the findings of Weishaupt et al who reported that the beneficial periodontal regeneration after treatment with EMD may be related to increased expression of the mineralization markers BSP and OPN at the mRNA level 31…”

“…Cementogenesis is a critical event in the repair of endodontic perforations, and EMD has been reported to induce cementogenesis when used for periodontal disease 13. The findings in our other study confirmed the capacity of MTA mixed with EMD to accelerate cementoblastic activity more than MTA alone and that EMD significantly stimulates the mineralization of human cementum-derived cells 14. The results of several previous studies have also suggested the beneficial effects of BMP-2 and EMD on hard tissue repair and regeneration.…”

“… 13 The findings in our other study confirmed the capacity of MTA mixed with EMD to accelerate cementoblastic activity more than MTA alone and that EMD significantly stimulates the mineralization of human cementum-derived cells. 14 The results of several previous studies have also suggested the beneficial effects of BMP-2 and EMD on hard tissue repair and regeneration. However, to the best of our knowledge, there has been no study comparing the combined effect of MTA/BMP-2 and MTA/EMD.…”

Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells

“…In addition, the use of EMD to promote pulp stem cell proliferation and hard tissue regeneration in endodontic dentistry has recently been reported [26][27][28][29][30]. Conventionally, EMD was considered to be a material with great potential to enhance the regeneration of periodontal tissue [22][23][24][25]. In this study, we compared the biocompatibility and osteogenic potential of ProRoot MTA, RetroMTA, and Endocem MTA Premixed combined with Emdogain on hBMSCs.…”

“…Enamel matrix derivative (EMD) is mostly composed of amelogenin and is known to contain growth factors. It has demonstrated significant potential in the regeneration of periodontal defects when applied to the root surface during periodontal surgery [22][23][24][25]. There are many recent reports regarding the use of Emdogain Gel (Straumann, Basel, Switzerland) in endodontics [26][27][28][29][30].…”