Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate into Motor Neurons on Electrospun Poly(ε-caprolactone) Scaffold

Abstract

Human endometrial and bone marrow-derived mesenchymal stem cells can be differentiated into a number of cell lineages. Mesenchymal stem cells (MSCs) are potential candidates for cellular therapy. The differentiation of human bone marrow MSCs (hBM-MSCs) and endometrial stem cells (hEnSCs) into motor neuron-like cells has been rarely investigated previously; however, the comparison between these stem cells when they are differentiated into motor neuron-like cell is yet to be studied. The aim of this study was therefore to investigate and compare the capability of hBM-MSCs and hEnSCs cultured on tissue culture polystyrene (TCP) and poly ε-caprolactone (PCL) nanofibrous scaffold to differentiate into motor neuron-like cells in the presence of neural inductive molecules. Engineered hBM-MSCs and hEnSCs seeded on PCL nanofibrous scaffold were differentiated into beta-tubulin III, islet-1, Neurofilament-H (NF-H), HB9, Pax6, and choactase-positive motor neurons by immunostaining and real-time PCR, in response to the signaling molecules. The data obtained from PCR and immunostaining showed that the expression of motor neuron markers of both hBM-MSCs and hEnSCs differentiated cells on PCL scaffold are significantly higher than that of the control group. The expression of these markers in hEnSCs differentiated cells was higher than that in hBM-MSCs. However, this difference was not statistically significant. In conclusion, differentiated hBM-MSCs and hEnSCs on PCL can provide a suitable three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system. Both cells may be potential candidates for cellular therapy in motor neuron disorders. However, differentiation of hEnSCs into motor neuron-like cells was better than hBM-MSCs.

Critical‐sized full‐thickness skin defect regeneration using ovine small intestinal submucosa with or without mesenchymal stem cells in rat model

“…Suitable cells for tissue engineering should be easily cultured and adhere . Flow cytometry analyses of these cells showed that CD73, CD105, CD29, CD44, and CD90 were highly expressed in BM‐MSCs while the expression of BM‐MSCs surface antigens, such as CD45 and CD34, were extremely low.…”

“…They have been in the spotlight of the regenerative medicine because of their various remarkable properties, such as self‐renewal ability, immunomodulatory properties, strong secretory properties, and their ability to differentiate into various epidermal cell types, such as keratinocytes and fibroblasts as well as dermal appendixes including, apocrine glands or hair follicle. It has also been reported that increase in basic fibroblast growth factor (b‐FGF), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) levels are induced by MSCs . Local transplantation of rat adipose‐derived MSCs to an excisional wound‐healing model revealed enhanced granulation tissue deposition and epithelialization …”

Comparison of Cell Proliferation and Adhesion of Human Osteoblast Differentiated Cells on Electrospun and Freeze-Dried PLGA/Bioglass Scaffolds

“…Stem cells may be extracted from different origins, like adipose tissue, bone marrow, muscle, periosteum, and placenta (26)(27)(28)(29). Endometrial stem cells are in fact a group of stem cells (adult stem cell), which are extracted from endometrial tissue of the uterus, which present some features similar to mesenchymal stem cells.…”

The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine

“…Cultured endometrial MSCs usually express typical BM-MSC markers, such as CD90, CD105, CD146 28 , CD9, CD13, CD14, CD29, CD31, CD44, CD73, CD117 and CD133 18 , but they do not express other markers, such as STRO 1, CD31 (typical of endothelial origin cells), or CD34, from haematopoietic stem cells (HSCs) and endothelial cells. 29 General adult stem cell markers, such as bcl-2, c-kit (CD117) and CD34, have been identified in human endometrial tissue.…”

“…28,92,93 Therefore, these cells might be used to treat affections such as cardiac disease, muscle degeneration, diabetes type 1 and neurodegenerative diseases in the future.…”