Comparison of Catabolic Rates of &lt;i&gt;sn&lt;/i&gt;-1, &lt;i&gt;sn&lt;/i&gt;-2, and &lt;i&gt;sn&lt;/i&gt;-3 Fatty Acids in Triacylglycerols Using &lt;sup&gt;13&lt;/sup&gt;CO&lt;sub&gt;2&lt;/sub&gt; Breath Test in Mice

Beppu, Fumiaki; Kawamatsu, Takashi; Yamatani, Yoshio; Nagai, Toshiharu; Yoshinaga, Kazuaki; Mizobe, Hoyo; Yoshida, Akihiko; Kubo, Atsushi; Kanda, Jun; Gotoh, Naohiro

doi:10.5650/jos.ess16124

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…These results indicate that palmitic acid or oleic acid bound to the b position of TAG can be catabolized for a longer duration. These trends are consistent with those reported in our studies comparing the catabolic rates of 13 C-labeled palmitic acid or oleic acid at the α and b positions 17,18 . Our previous studies showed that palmitic acid or oleic acid bound to the α position is quickly catabolized, whereas palmitic acid or oleic acid bound to the b position is slowly catabolized for a long time.…”

Section: Discussionsupporting

confidence: 92%

“…However, the rates gradually changed, and 4-6 hours after administration of labeled TAG, the catabolic rate of the fatty acid at the b position was higher than that of the fatty acid at the α position. Furthermore, we compared the catabolic rates of 13 C-labeled palmitic acid or oleic acid at the sn-1, sn-2, and sn-3 positions of TAG 18 . We found differences between the α sn-1 and sn-3 position and b sn-2 position in all experiments, and the fatty acids at the α position were catabolized faster than those at the b position.…”

Section: Introductionmentioning

confidence: 99%

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Examination of the Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using 13CO2 Expired from Mice

et al. 2019

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Fatty acids in triacylglycerol (TAG) are catabolized after digestion. However, the catabolic rates of several fatty acids bound to the α (sn-1, 3) or β (sn-2) position of TAG have not been thoroughly compared. In this study, the catabolic rates of 13 C-labeled palmitic acid, oleic acid, linoleic acid, α-linolenic acid, eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA) bound to the α and β position of TAG were compared using isotope ratio mass spectrometry. The catabolic rates of the studied fatty acids were evaluated using the ratio of 13 C and 12 C in carbon dioxide expired from mice. The results indicated that palmitic acid, oleic acid, or α-linolenic acid bound to the β position was slowly catabolized for a long duration compared to that when bound to the α position. In contrast, EPA bound to the β position was quickly catabolized, and EPA bound to the α position was slowly catabolized for a long time. For linoleic acid or DHA, no difference in the catabolic rates was detected between the binding positions in TAG. Furthermore, EPA and DHA were less catabolized than the other fatty acids. These results indicate that the catabolic rates of fatty acids are influenced by their binding positions in TAG and that this influence on the catabolic rate differed depending on the fatty acid species.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Examination of the Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using 13CO2 Expired from Mice

et al. 2019

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“…The results showed that catabolic rate was different between α and β positions and that in the first stage, the palmitic and oleic acids at α position were more quickly catabolized than that at β position. Thereafter, the rates changed gradually, and at 4-6 hours after administration of labeled TAG, the catabolic rate of the palmitic and oleic acids at β position became higher than that of these acids at the α position 18 . However, our recent study comparing the catabolic rates of 13 C-labeled palmitic acid, oleic acid, and linoleic acid at α and β positions of TAG showed that there was a slight difference in the catabolic rates of 13 C-labeled fatty acids between the binding positions of TAG 19 .…”

Section: Discussionmentioning

confidence: 99%

Effect of Calcium Treatment on Catabolic Rates of 13C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol

et al. 2019

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The absorption efficacies and catabolic rates of fatty acids are affected by their binding position on triacylglycerol (TAG). However, the kind of effect calcium treatment has on the catabolism of fatty acids is unclear. In this study, the catabolic rates of 13 C-labeled palmitic acid, oleic acid, and linoleic acid bound to sn-1, 3 (α) and sn-2 (β) position of TAG in the presence of calcium were compared using isotope ratio mass spectrometry. The catabolic rates of 13 C-labeled fatty acids were evaluated using the ratio of 13 C to 12 C in the carbon dioxide expired by mice. The catabolic rate of palmitic acid bound to the α position was significantly lower than that of palmitic acid bound to the β position of TAG. The rates of 13 CO 2 formation from palmitic acid at the β position remained higher for a long time. In contrast, oleic and linoleic acids at the α position were as well catabolized as those at the β position. These results indicate that in the presence of calcium, the saturated fatty acid bound to the β position is highly catabolized, whereas that bound to the α position is not well catabolized. Saturated fatty acid at the α position is hydrolyzed by pancreatic lipase to promptly form insoluble complexes with calcium, which are excreted from the body, and thereby reducing the catabolic rate of these fatty acids.

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“…In addition, the effect of the binding position of fatty acids in TAG on their catabolic rates using a CO 2 breath test in mice was investigated. The catabolic rates of 1-13 C-labeled P or O at the sn-1, sn-2, and sn-3 positions of TAG were compared in our previous study 42 . Differences were observed between the αsn-1 and sn-3 and βsn-2 position, and the fatty acids at the α position were for a short while catabolized faster than those at the β position after administration.…”

Section: Metabolism Of Triacylglycerol Positional Isomersmentioning

confidence: 99%

Comparison of Catabolic Rates of <i>sn</i>-1, <i>sn</i>-2, and <i>sn</i>-3 Fatty Acids in Triacylglycerols Using <sup>13</sup>CO<sub>2</sub> Breath Test in Mice

Cited by 4 publications

References 20 publications

Examination of the Catabolic Rates of <sup>13</sup>C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using <sup>13</sup>CO<sub>2</sub> Expired from Mice

Examination of the Catabolic Rates of <sup>13</sup>C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol Using <sup>13</sup>CO<sub>2</sub> Expired from Mice

Effect of Calcium Treatment on Catabolic Rates of <sup>13</sup>C-Labeled Fatty Acids Bound to the α and β Positions of Triacylglycerol

Development of Analytical Methods and Nutritional Studies Using Synthetic Fatty Acids and Triacylglycerols

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