Comparison of catheter thrombogenicity in a modified chandler loop model using goat blood

Olsen, Aaron L.; Long, Michael T.

doi:10.1002/jbm.a.36509

Cited by 5 publications

(6 citation statements)

References 41 publications

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“…While the above study established the framework of the dynamic test system and also provided a new static thrombosis pre‐test method to estimate donor‐specific anticoagulation levels, several test conditions (e.g., blood species, test temperature, and test duration) that could impact thrombosis require further investigation to broaden our knowledge in this area. Several in vitro flow loop thrombogenicity test systems have been described by other research groups 9,13–24 . However, currently there are no widely accepted or standardized methods.…”

Section: Introductionmentioning

confidence: 99%

“…9,13-24 However, currently there are no widely accepted or standardized methods. Although different types of animal blood have been used as a substitute for human blood in flow loop thrombogenicity tests, 9,[13][14][15][16][17][18][19][20][21][22][23][24] and some studies have compared blood properties of different species under various experimental settings, [25][26][27][28][29][30][31][32] these studies did not evaluate whether it is appropriate or acceptable to use animal blood instead of fresh human blood for in vitro dynamic thrombogenicity testing. To address this issue, the current study aimed to compare and evaluate the ability of blood from humans and four common animal sources (ovine, bovine and porcine blood from live donors, and abattoir porcine blood) to differentiate materials based on their relative thrombogenicity potentials in our dynamic in vitro thrombogenicity test system.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials

et al. 2022

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Background To determine suitable alternatives to human blood for in vitro dynamic thrombogenicity testing of biomaterials, four different animal blood sources (ovine, bovine, and porcine blood from live donors, and abattoir porcine blood) were compared to fresh human blood. Methods To account for blood coagulability differences between individual donors and species, each blood pool was heparinized to a donor‐specific concentration immediately before testing in a dynamic flow loop system. The target heparin level was established using a static thrombosis pre‐test. For dynamic testing, whole blood was recirculated at room temperature for 1 h at 200 ml/min through a flow loop containing a single test material. Four materials with varying thrombotic potentials were investigated: latex (positive control), polytetrafluoroethylene (PTFE) (negative control), silicone (intermediate thrombotic potential), and high‐density polyethylene (HDPE) (historically thromboresistant). Thrombus weight and surface area coverage on the test materials were quantified, along with platelet count reduction in the blood. Results While donor‐specific heparin levels varied substantially from 0.6 U/ml to 7.0 U/ml among the different blood sources, each source was able to differentiate between the thrombogenic latex and the thromboresistant PTFE and HDPE materials (p < 0.05). However, only donor ovine and bovine blood were sensitive enough to differentiate an increased response for the intermediate thrombotic silicone material compared to PTFE and HDPE. Conclusions These results demonstrated that multiple animal blood sources (particularly donor ovine and bovine blood) may be suitable alternatives to fresh human blood for dynamic thrombogenicity testing when appropriate control materials and donor‐specific anticoagulation levels are used.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials

et al. 2022

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“…To analyze coagulation characteristics of the printed BG SA-COP , two established models were used: first, coagulation activation and solid thrombus formation by whole blood was investigated in the dynamic Chandler Loop thrombosis model and second platelet activation was analyzed by applying platelet rich plasma (PRP) in a platelet-activation-assay. [38] After 30 min in the chandler loop, only the BG SA showed distinct thrombus formation indicating a higher thrombogenicity than the other experimental groups. After 60 min thrombi occurred in all four experimental groups.…”

Section: Coagulation Activation and Thrombogenicitymentioning

confidence: 84%

“…To analyze coagulation characteristics of the printed BG SA‐COP , two established models were used: first, coagulation activation and solid thrombus formation by whole blood was investigated in the dynamic Chandler Loop thrombosis model and second platelet activation was analyzed by applying platelet rich plasma (PRP) in a platelet‐activation–assay. [ 38 ]…”

Section: Resultsmentioning

confidence: 99%

4D Printing of Bioartificial, Small‐Diameter Vascular Grafts with Human‐Scale Characteristics and Functional Integrity

Pfarr,

Zitta,

Hummitzsch

et al. 2024

Adv Materials Technologies

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A novel developed 4D bioprinting technique is used for the manufacturing of human‐scale, small‐diameter vascular grafts. Accordingly, a bio‐ink is synthesized from a hybrid molecule containing sodium alginate (SA) and collagen peptide (COP). Endothelial progenitor cells (EPC) isolated from human whole blood are integrated into the bioartificial vascular graft as an autologous cell source. Likewise, human umbilical vein endothelial cells (HUVEC) are used as experimental standard. The evolving vascular grafts are printed by a customized 4D bioprinter into CaCl2 support medium for rapid cross‐linking inducing the temporospatial shaping of the grafts. After culturing for 21 days, histological and ultrastructural analyses of the bioartificial vascular grafts reveal a well‐organized matrix with imbedded EPC or HUVEC. Live‐3D‐cell–imaging and cell viability assays demonstrate a multitude of vital and metabolically active cells. Biomechanics of the grafts are proven to be comparable to human saphenous veins. Coagulation analysis reveals low thrombogenicity and high functional integrity of the vascular grafts. Surgical implantation of the grafts in a perfused cadaver model can be performed effectively and without technical issues. Finally, the current study describes for the first time the 4D bioprinting and characterization of a small‐diameter, human‐scale vascular graft for putative clinical translation.

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“…Several in vitro dynamic thrombogenicity test methods have been developed to evaluate the thrombogenic potentials of materials and medical devices. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] However, due to the lack of standardized test protocols and the inherent variability of blood, the results produced by these dynamic flow systems tend to differ greatly. 12,25 In our lab, we previously developed an in vitro dynamic blood flow system in which the relative thrombogenic potentials of multiple materials with catheterlike geometries were effectively differentiated by circulating ovine blood through a flow loop at room temperature (RT) for 1 hour.…”

mentioning

confidence: 99%

Effect of Temperature on Thrombogenicity Testing of Biomaterials in an In Vitro Dynamic Flow Loop System

et al. 2023

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To develop and standardize a reliable in vitro dynamic thrombogenicity test protocol, the key test parameters that could impact thrombus formation need to be investigated and understood. In this study, we evaluated the effect of temperature on the thrombogenic responses (thrombus surface coverage, thrombus weight, and platelet count reduction) of various materials using an in vitro blood flow loop test system. Whole blood from live sheep and cow donors was used to assess four materials with varying thrombogenic potentials: negative-control polytetrafluoroethylene (PTFE), positivecontrol latex, silicone, and high-density polyethylene (HDPE). Blood, heparinized to a donor-specific concentration, was recirculated through a polyvinyl chloride tubing loop containing the test material at room temperature (22-24°C) for 1 hour, or at 37°C for 1 or 2 hours. The flow loop system could effectively differentiate a thrombogenic material (latex) from the other materials for both test temperatures and blood species (p < 0.05). However, compared with 37°C, testing at room temperature appeared to have slightly better sensitivity in differentiating silicone (intermediate thrombogenic potential) from the relatively thromboresistant materials (PTFE and HDPE, p < 0.05). These data suggest that testing at room temperature may be a viable option for dynamic thrombogenicity assessment of biomaterials and medical devices. ASAIO

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Comparison of catheter thrombogenicity in a modified chandler loop model using goat blood

Cited by 5 publications

References 41 publications

Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials

Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials

4D Printing of Bioartificial, Small‐Diameter Vascular Grafts with Human‐Scale Characteristics and Functional Integrity

Effect of Temperature on Thrombogenicity Testing of Biomaterials in an In Vitro Dynamic Flow Loop System

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