Comparison of Cecal Microbiota and Performance Indices Between Lean-Type and Fatty-Type Pekin Ducks

Abstract

Fatty-type (FT) Pekin ducks exhibit higher lipid deposition than lean-type (LT) ducks. The gut microbiota plays an important role in modulating fat metabolism. We compared the growth performance, slaughter performance, and cecal microbiota of FT and LT Pekin ducks and analyzed the role of cecal microbiota in lipid deposition in Pekin ducks. A total of 140 1-day-old FT and LT Pekin ducks with similar body weights were randomly assigned to 10 cages, with 14 ducks in each replicate. All ducks were fed commercial diets from 28 to 42 days of age. Results showed that the average body weight and feed intake of FT ducks were higher than those of LT ducks. The breast muscle and eviscerated percentages of LT ducks were higher than those of FT ducks; the abdominal fat and sebum percentages of LT ducks were lower than those of FT ducks at 6 weeks of age (P < 0.01). 16S DNA sequencing of the cecal microbiota revealed that the bacterial abundance differed between FT and LT ducks at 4 and 6 weeks of age. The abundance of Firmicutes was higher, while that of Fusobacteria and Fusobacterium was lower in LT ducks than in FT ducks at 4 weeks of age. The abundance of Spirochaetes was higher, while that of Firmicutes and Bacteroides was lower in LT ducks than in FT ducks at 6 weeks of age. The abundance of Spirochaetes and Brachyspira in LT ducks was higher at 6 weeks than at 4 weeks of age. Interestingly, the abundance of Firmicutes and Bacteroides in FT ducks was higher at 6 weeks of age than at 4 weeks of age, while that of Fusobacteria and Fusobacterium was lower at 6 weeks than at 4 weeks of age. Linear discriminant analysis effect size analysis showed that Spirochaetes, Brachyspira, Alistipes, Campylobacter, Megamonas, Butyricicoccus, and Fusobacteria may be involved in the fat metabolism pathway as specific markers. We reveal the differences in microbial abundance in the cecal microbiota between FT and LT Pekin ducks and provide an insight into the role of cecal microbiota in lipid deposition in Pekin ducks.

Distribution of bacteria in different regions of the small intestine with Zanthoxylum bungeanum essential oil supplement in small-tailed Han sheep

“…Relative abundances of Firmicutes, Bacteroidetes, Tenericutes, and Proteobacteria increased significantly in DLW, CLW, BLW, and ALW groups than in the other groups (p < 0.001, p < 0.001, p = 0.002, and p = 0.001, respectively). Spirochaetes were closely associated with fat metabolism pathways (Yang et al, 2022) and can degrade pectin and xylan (Liu et al, 2014;Tokuda et al, 2018). Spirochaetes was the dominant bacteria in this study, but the difference in relative abundance between groups was not significant.…”

Association of residual feed intake with intestinal microbiome and metabolome in laying period of ducks

“…The abundance of Anaerobiospirillum was significantly positively correlated with Norsanguinarine, (3-[4-[2-3.5,7trihydroxy-6-3,4-dihydro-2H-1-benzopyran-2-yllphenyl) oxidanesulfonic acid, (−)-11-hydroxy-9,10-dihydrojasmonic acid 11-beta-Dglucoside and (4-{2-hexatriaconta-16,24,26,28-tetraen-12-yl] propyl}-2-hydroxycyclohexyl) oxidanesulfonic acid Dextrin D-Ribose. Anaerobiospirillum may reduce fat deposition (Li et al, 2022), and studies have found that it is significantly enriched in the cecum of lean ducks compared to Peking ducks (Yang et al, 2022). Lactobacillus can improve the feed efficiency of chickens (Gao et al,FIGURE 6 The rectal metabolomics pathway of two groups of laying ducks with different residual feed intake was analyzed.…”

Effects of cage versus floor rearing system on goose intestinal histomorphology and cecal microbial composition

“…A study found that the abundance of Megamonas in male chickens was significantly positively correlated with glycogen phosphorylase L ( PYGL ) expression in the cecum ( Cui et al, 2021 ), and the PYGL gene was involved in glycan metabolism. Compared to fatty-type ( FT ) Pekin ducks, Anaerobiospirillum has significantly enriched in the cecum of lean-type ( LT ) ducks ( Yang et al, 2022 ). In this study, we also found that the relative abundance of Megamonas (R = −0.52 and R = −0.51) and Anaerobiospirillum (R = −0.58 and R = −0.60) were significantly negatively correlated with abdominal fat weight and relative abdominal fat weight ( P < 0.01).…”