Comparison of Chalquest and Hayflick media, with and without ammonium reineckate, for isolating Mycoplasma pulmonis from rats

Lai, Wayne C.; Pakes, S. P.; Stefanu, C; Lu, Y S

doi:10.1128/jcm.23.5.817-821.1986

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…Modified Hayflick medium is recommended for the isolation of M. bovis in milk samples (Pfützner & Sachse 1996) and successfully used by several authors (Pretto et al 2001, Cai et al 2005, Riekerink et al 2006. The use of more than one culture medium may increase the isolation rate of Mycoplasma spp., as observed by Lai et al (1986), when comparing Chalquest and Hayflick's means for detecting M. pulmonis, with 92% and 66% bacterial isolation, respectively. Tortorelli et al (2017) obtained only positive isolation on Hayflick medium in 32 sections of bovine respiratory disease, stressing a low percentage when compared to other studies.…”

Section: Microbiological Analysismentioning

confidence: 57%

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

et al. 2020

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The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil.

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Section: Microbiological Analysismentioning

confidence: 57%

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

et al. 2020

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“…We used the T2 strain, which has been described before (13). Twenty mice (10 female and 10 male) of the B6 and C3H (16). Lung suspensions were made after the lungs were aseptically removed and homogenized in 1 ml of Chalquest's broth plus ammonium reineckate in a tissue grinder (Fisher, Pittsburgh, Pa.).…”

Section: Methodsmentioning

confidence: 99%

Genetic control of resistance to Mycoplasma pulmonis infection in mice

et al. 1993

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The differences in susceptibility of various inbred strains of mice to a highly pathogenic strain ofMycoplasma pulmonis CT (T2) has been known for some time. We assessed the genetic control of resistance to T2 infection. Tracheolung lavage samples and lungs of mice were assessed for T2 organisms after intratracheal injection of T2. We found that H-2b C57BL/6 (B6) and H-2k B1O.BR mice were resistant, whereas H-2b A.By, H-2k C3HIBi, H-2k C3H/HeJ (C3H), and H-2b BALB.B mice were susceptible. We also typed individual B6C3F2 mice for H-2 and for resistance to T2 and observed that resistance to T2 infections is controlled by a single dominant gene not linked to H-2. Histologic examination revealed severe lung lesions typical of M. pulmonis infections in susceptible C3H mice, in contrast to minimal lung lesions in resistant B6 mice. No significant titers of local or systemic antimycoplasma antibodies were detected in either resistant or susceptible mice at 5 days postinfection. Macrophages taken from uninfected B6 or C3H mice failed to inhibit growth of T12 in vitro. However, macrophages from B6 mice did inhibit growth of T2 much better than C3H macrophages when harvested on day 5 of infection. Thus, there is an association between activation of macrophage bactericidal function and genetic resistance to growth of T2 organisms.

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“…Mycoplasma isolation, titration, and identification. The procedures for mycoplasma isolation, titration, and identification were modified as described previously (20). We used a macrotitration method; 0.1-ml samples were cultured in Chalquest agar medium.…”

Section: Methodsmentioning

confidence: 99%

Vaccination of Lewis rats with temperature-sensitive mutants of Mycoplasma pulmonis: adoptive transfer of immunity by spleen cells but not by sera

Lai

Bennett

et al. 1991

Infect Immun

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Comparison of Chalquest and Hayflick media, with and without ammonium reineckate, for isolating Mycoplasma pulmonis from rats

Cited by 10 publications

References 13 publications

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Genetic control of resistance to Mycoplasma pulmonis infection in mice

Vaccination of Lewis rats with temperature-sensitive mutants of Mycoplasma pulmonis: adoptive transfer of immunity by spleen cells but not by sera

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