Comparison of Chironomus usenicus and Chironomus curabilis with species of the group plumosus (Diptera) inferred from the mitochondrial DNA Gene COI and by the polytene chromosomes banding pattern

Polukonova, N. V.; Djomin, A. G.; Mugue, N. S.; Шайкевич, Е. В.

doi:10.1134/s102279540908002x

Cited by 13 publications

(15 citation statements)

References 7 publications

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“…; Polukonova et al . ). There were also particular species that magnified the overlap, and it is worth re‐examining those samples.…”

Section: Discussionmentioning

confidence: 97%

“…; Polukonova et al . ). Considering the wide habitat range of Oh‐yusurika in Japan, far more extensive sampling is needed, even though our collection sites were located in eastern and western Japan, and covered a wide area of Japan (Table ).…”

Section: Discussionmentioning

confidence: 97%

“…1, Tables 2,3). This overlap was partially caused by sibling species, such as the C. plumosus group, and a group of Chironomus tentans Fabricius, Chironomus pallidivittatus Malloch, Chironomus biwaprimus Sasa and Kawai, and Chironomus dilutus Shobanov et al (Martin et al 2002;Polukonova et al 2009). There were also particular species that magnified the overlap, and it is worth re-examining those samples.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is not known whether the clade of Japanese C. plumosus consisted of multiple species or not because of the lack of cytological data in this study and sequence data from reliable specimens confirmed by cytological analyses. In previous studies of C. usenicus, the COI gene sometimes afforded lower resolution than the karyological method for identifying the closest among sibling species of the C. plumosus group (Polukonova & Beljanina 2002;Golygina et al 2007;Polukonova et al 2009). Considering the wide habitat range of Oh-yusurika in Japan, far more extensive sampling is needed, even though our collection sites were located in eastern and western Japan, and covered a wide area of Japan (Table 1).…”

Section: Chironomus Plumosusmentioning

confidence: 99%

“…For the Chironomidae, there is a growing number of studies of DNA barcoding and phylogenetic analysis using DNA sequences (Guryev et al 2001;Martin et al 2002Martin et al , 2007Ekrem et al 2007Ekrem et al , 2010aPfenninger et al 2007;Sinclair & Gresens 2008;Polukonova et al 2009;Krosch et al 2011;Stur & Ekrem 2011;Cranston et al 2012;Kim et al 2012;Roongruangwongse et al 2012;Anderson et al 2013;Proulx et al 2013;Lin et al 2015) and attempts at aquatic biomonitoring using DNA sequences (Sharley et al 2004;Carew et al 2007Carew et al , 2011Brodin et al 2013). In these studies, the commonly used sequences were of the mitochondrial genes cytochromec oxidase subunit I (COI) and cytochromeb oxidase (cytb) and the nuclear genes globin 2β (gb2β), rudimentary (CAD) and ribosomal RNA (18S and 28S rRNA).…”

Section: Introductionmentioning

confidence: 99%

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DNA barcoding supports reclassification of JapaneseChironomusspecies (Diptera: Chironomidae)

Kondo

Ueno

Ohbayashi

et al. 2016

Entomological Science

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Non‐biting midges (Diptera: Chironomidae) adapt to species‐specific environmental conditions and hence are promising bioindicators for aquatic and ecotoxicological monitoring. Although their utility for these purposes was historically limited by difficulties in their morphological identification, DNA barcoding offers a possible solution. Here, eight Japanese species of the genus Chironomus, which is characterized by its worldwide distribution and abundance among Chironomidae, were subjected to DNA barcoding using cytochromec oxidase subunit I (COI). To examine whether this DNA barcode is a useful indicator for Japanese species of Chironomus, we calculated genetic distances within and between the COI sequences of Chironomus species both from this study and worldwide and constructed phylogenetic trees. Based on 415 bp COI sequences and the Kimura two‐parameter model, the average genetic distances within 37 species and between 72 species were 2.6% and 17.2%, respectively. Although the ranges of genetic distances within and between species overlapped from 0.8% to 17.3%, 99.7% of average genetic distances between species were >3.0%. Some of this overlap is attributable to distances within species that were “too large” as well as those between species that were “too small”. Of eight Japanese species examined, two showed genetic distances between species that were below a 3.0% threshold, and four had distances within species that were greater than 3.0%. These results suggest a possible reclassification of these species and the need for further sampling to unveil biogeographic variations among different countries and regions.

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“…; Polukonova et al . ). There were also particular species that magnified the overlap, and it is worth re‐examining those samples.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Chironomus Plumosusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DNA barcoding supports reclassification of JapaneseChironomusspecies (Diptera: Chironomidae)

Kondo

Ueno

Ohbayashi

et al. 2016

Entomological Science

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DNA barcoding of Chironomidae from the Lake Skadar region: Reference library and a comparative analysis of the European fauna

Gadawski

Montagna

Rossaro

et al. 2022

Diversity and Distributions

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Aims:The main aim of this study was to fill a gap in barcoding data of the European Chironomidae of the Balkan region, developing and testing the efficiency of a reference DNA barcode library for ancient Lake Skadar basin species (Montenegro/Albania), a region of Europe never before subjected to barcoding studies on Chironomidae.Another aim was to test the efficiency of DNA barcoding for the identification of European Chironomidae, including the estimation of optimal identification thresholds, using >12,000 barcodes.Location: Lake Skadar basin and adjacent area (Montenegro/Albania).Methods: Through this study, 770 individuals of Chironomidae from the Lake Skadar region were barcoded, both at adult and pre-imaginal stages. Adults were morphologically identified, while larvae were assigned to species by molecular identification, using different methods, of which the efficiency was tested, for a total of 97 different barcoded species. Results:The identification efficiency of the reference dataset developed for the Lake Skadar region was 98.6%, a value in line with that obtained when the identification efficiency for European Chironomidae was evaluated (95.8%), which confirms the accuracy of DNA barcoding for the identification of these insects. Moreover, we found that the optimal threshold for the molecular identification of the family is 1.6% nucleotide distance, though more specific thresholds are suggested for the identification of species belonging to Chironomidae subfamilies, since they are related to lower identification errors than to the use of a general threshold. The analysis of inconsistency between molecular and morphological identification shed light on taxonomic issues within European Chironomidae. Previously postulated species synonyms were confirmed, and also further cases requiring deeper investigation were detected.Main conclusions: Our de novo DNA barcode library was shown to have a high identification efficiency. Taxon-specific thresholds increase the efficacy of molecular identification. Hypothesized species synonyms could be validated through molecular techniques.

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Microevolutionary changes in populations of Chironomus nuditarsis Str. (Keyl, 1962) (Chironomidae, Diptera) from central caucasus

Polukonova

Karmokov

2013

Russ J Genet

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Comparison of Chironomus usenicus and Chironomus curabilis with species of the group plumosus (Diptera) inferred from the mitochondrial DNA Gene COI and by the polytene chromosomes banding pattern

Cited by 13 publications

References 7 publications

DNA barcoding supports reclassification of JapaneseChironomusspecies (Diptera: Chironomidae)

DNA barcoding supports reclassification of JapaneseChironomusspecies (Diptera: Chironomidae)

DNA barcoding of Chironomidae from the Lake Skadar region: Reference library and a comparative analysis of the European fauna

Microevolutionary changes in populations of Chironomus nuditarsis Str. (Keyl, 1962) (Chironomidae, Diptera) from central caucasus

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