Comparison of chitosan, alginate and chitosan/alginate nanoparticles with respect to their size, stability, toxicity and transfection

Rafiee, Aras; Alimohammadian, Mohammad Hossein; Gazori, Taraneh; Riazi-Rad, Farhad; Fatemi, Seyed Mohammad Reza; Parizadeh, Amirabbas; Haririan, Ismaeil; Havaskary, M.

doi:10.1016/s2222-1808(14)60590-9

Cited by 57 publications

(31 citation statements)

References 25 publications

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“…Further supporting this hypothesis, we observed that CS/alginate nanoparticles mediated cellular uptake of antisense better than naked antisense in T47D cells . CS and CS/alginate nanoparticles showed better capacity to load pDNA and had the higher transfection efficacy into HEK293 cells than alginate nanoparticles .…”

Section: Polysaccharide Nanoparticlessupporting

confidence: 70%

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Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

246

143

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Nanoparticulate drug/gene carriers have gained much attention in the past decades because of their versatile and tunable properties. However, efficacy of the therapeutic agents can be further enhanced using naturally occurring materials‐based nanoparticles. Polysaccharides are an emerging class of biopolymers; therefore, they are generally considered to be safe, non‐toxic, biocompatible and biodegradable. Considering that the target of nanoparticle‐based therapeutic strategies is localization of biomedical agents in subcellular compartments, a detailed understanding of the cellular mechanism involved in the uptake of polysaccharide‐based nanoparticles is essential for safe and efficient therapeutic applications. Uptake of the nanoparticles by the cellular systems occurs with a process known as endocytosis and is influenced by the physicochemical characteristics of nanoparticles such as size, shape and surface chemistry as well as the employed experimental conditions. In this study, we highlight the main endocytosis mechanisms responsible for the cellular uptake of polysaccharide nanoparticles containing drug/gene.

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Section: Polysaccharide Nanoparticlessupporting

confidence: 70%

“…Actually, not only alginate exhibits ‘proton sponge effect’, but also its degradation promotes the osmotic pressure, thereby increasing the endosomal release of cargo. Besides, swelling properties of the alginate owing to hydrogel property may mediate the cellular uptake and the release of pDNA into the cytosol .…”

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

246

143

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“…This peak value was interpreted as an interaction between the two polymers [24]. XRD spectra of PEG nanoparticles have shown the presence of 2 peaks of SA and a large bump.…”

Section: Resultsmentioning

confidence: 97%

Novel Nanoparticles for the Oral Delivery of Low Molecular Weight Heparin: In Vitro and in Vivo Assessment

Nallaguntla

Muzib

Aukunuru³

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of the present study was to prepare and evaluate a novel oral formulation of nanoparticles for the systemic delivery of lowmolecular-weight heparin (LMWH).Methods: Nanoparticles were prepared by polyelectrolyte complexation method using polymers, i.e., sodium alginate and chitosan (CH). Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD), entrapment efficiency, In vitro release and scanning electron microscopic studies were carried out for nanoparticles. Ex vivo permeation studies were performed with optimized formulation using small intestine of rat and in vivo studies were conducted on rat model. Results:Entrapment efficiency of LMWH in nanoparticles was found to be 88%. In vitro release studies demonstrated that the release of LMWH was negligible in the stomach and high in the small intestine. FTIR has indicated that there is no interaction between the ingredients in nanoparticle. DSC and XRD studies confirmed that the amino groups of CH interacted with the carboxylic groups of alginate. In vitro % drug release of 95% was shown by formulation AC5. Ex vivo permeation studies have elucidated that ~73% of LMWH was transported across the epithelium. Nanoparticles have shown enhanced oral bioavailability of LMWH as revealed by 4.5-fold increase in area under the curve of plasma drug concentration-time curve. Conclusion:The results suggest that the nanoparticles prepared can result in targeted delivery of LMWH into systemic circulation through intestinal and colon routes. Novel nanoparticles thus prepared in this study can be considered as a promising delivery system.

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“…Coating of chitosan with anionic alginate reduces the interaction between chitosan and DNA, thereby mediating the dissociation of DNA upon entry into cell. 23,79 Therefore, encapsulated p-DNA and siRNA can be preserved from nuclease degradation without being damaged. In addition, chitosan nanoparticles may be more efficient in delivering encapsulated siRNA into target cells as compared with liposomes.…”

Section: Polysaccharide Nanoparticles For Gene Deliverymentioning

confidence: 99%

Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery

Salatin¹,

Jelvehgari²

2017

Pharm Sci

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Comparison of chitosan, alginate and chitosan/alginate nanoparticles with respect to their size, stability, toxicity and transfection

Cited by 57 publications

References 25 publications

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Novel Nanoparticles for the Oral Delivery of Low Molecular Weight Heparin: In Vitro and in Vivo Assessment

Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery

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Comparison of chitosan, alginate and chitosan/alginate nanoparticles with respect to their size, stability, toxicity and transfection

Cited by 57 publications

References 25 publications

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Novel Nanoparticles for the Oral Delivery of Low Molecular Weight Heparin: In Vitro and in Vivo Assessment

Natural Polysaccharide based Nanoparticles for Drug/Gene Deliv­ery

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Product

Resources

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Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery