Comparison of chromatographic ion-exchange resins

Staby, Arne; Jensen, Inge Holm; Mollerup, Inger

doi:10.1016/s0021-9673(00)00780-9

Cited by 77 publications

(18 citation statements)

References 27 publications

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“…Q Ceramic HyperD and Poros 50 D are rigid resins specifically designed for use at high flow rates tolerating 200 bar (Q Ceramic HyperD) or 100 bar (Poros 50 D), respectively. For these two resins, very little differences between dynamic capacities at low and high flow rates have been reported previously [19,20].…”

Section: Chromatographic Breakthrough Studiesmentioning

confidence: 57%

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

et al. 2008

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This study evaluates the applicability of the liquid handling workstation Tecan Evo Freedom 200 and 200 lL Media Scout RoboColumns to dynamic chromatographic operations such as frontal analysis (breakthrough) and elution experiments in a high throughput screening mode. Breakthrough experiments were conducted using BSA as a model protein and the stationary phases Poros 50 D, Q Ceramic HyperD and DEAE Sepharose FF. The obtained dynamic capacities at 10 and 50 % breakthrough matched well with reference data. Elution experiments were performed applying three protein mixtures: a) lipolase and BSA, b) human growth hormone and a process-related impurity, and c) an insulin analogue and a process-related impurity. The resins used resembled a variety of different ion exchange resins. In all cases, the resulting elution curves matched well with reference measurements performed on an ÄKTA explorer system at 1 mL or 2 mL scale.

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Section: Chromatographic Breakthrough Studiesmentioning

confidence: 57%

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

et al. 2008

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“…It has been reported that the dynamic capacity (at 10% breakthrough) of the commercially available biporous polymer-based anion-exchange resin POROS QE/M for BSA was 38 mg mL 1 at a flow rate of 720 cm h 1; this is lower than those of TSK-Gel-Q-5PW-HR, Express-Ion Q, and Q Sepharose FF (42, 50, and 54 mg mL 1, respectively) [22]. Obviously, Resin B has a dynamic binding capacity higher than that of this biporous resin and TSK-Gel-Q-5PW-HR, and comparable with those of Express-Ion Q and Q Sepharose FF.…”

Section: Dynamic Binding Capacilymentioning

confidence: 87%

“…It has been reported that plate heights for lysozyme of columns packed with 21.81xm POROS 20SP and 26.6 ixm SP POROS 20 OH beads under unretained conditions were 0.87 and 1.20 mm, respectively, at 1222 cm h 1 [16], and that the value for myoglobin of the column packed with 20 ixm POROS QE/M beads was approximately 2 mm at 720 cm h 1 [22]. The results presented here all indicate that column efficiency for Resin B was comparable with those of the commercially available biporous polymeric media of smaller particle size.…”

Section: Column Efficiencymentioning

confidence: 98%

Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

Shi

Sun

2003

Chromatographia

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SummaryA novel rigid spherical biporous poly(glyciclyl methacrylate-triallyl isocyanurate-clivinyJbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene and n-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity ancl column efficiency at high flow rates.

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“…h 1 for BSA are 38, 21, 28 and 38mg.mL 1, respectively [21]. Obviously, the dynamic capacity of Resin C is higher than that of Macro-Prep 25Q and Fractogel EMD TMAE 650s, and comparable to that of POROS QE/M, the typical commercial biporous anion-exchange resin, and Source 30Q.…”

Section: Adsorption Capacitymentioning

confidence: 88%

Development of rigid biporous polymeric adsorbent for protein chromatography

2002

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SummaryA novel biporous poly (glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted as Resin C) was prepared by in-situ co-polymerization with both superfine granules of sodium sulfate and the solvents toluene and n-heptane as porogenic agents. This material is based on a novel porogenic mode of a combination of both solid granules and solvents. The properties of Resin C were characterized, and then compared with both Resin A (where only solvents as porogen) and Resin B (where only solid granules are used as porogen). The biporous resin showed good mechanical performance, high dynamic adsorption capacity and column efficiency at high flow-rates. These factors indicate the biporous resin as a promising adsorbent for high-speed protein separation by chromatography.

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Comparison of chromatographic ion-exchange resins

Cited by 77 publications

References 27 publications

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

Development of rigid biporous polymeric adsorbent for protein chromatography

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