Comparison of Clinical Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing guidelines for the interpretation of antibiotic susceptibility at a University teaching hospital in Nairobi, Kenya: a cross-sectional study

Kassim, Ali; Omuse, Geoffrey; Premji, Zul; Revathi, Gunturu

doi:10.1186/s12941-016-0135-3

Cited by 105 publications

(93 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strains were identified by classical bacteriological diagnosis and the antimicrobial testing was performed by disc diffusion according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) (7). …”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance development following surgical site infections

Călina

Docea

Rosu

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Surgical site infections (SSIs) determine an increase in hospitalization time and antibiotic therapy costs. The aim of this study was to identify the germs involved in SSIs in patients from the Clinical Emergency County Hospital of Craiova (SCJUC) and to assess their resistance to antimicrobials, with comparisons between surgical wards and the intensive care unit (ICU). The biological samples were subjected to classical bacteriological diagnostics. Antibiotic resistance was tested by disc diffusion. We used hierarchical clustering as a method to group the isolates based upon the antibiotic resistance profile. The most prevalent bacterial species isolated were Staphylococcus aureus (S. aureus; 50.72%), followed by Escherichia coli (E. coli; 17.22%) and Pseudomonas aeruginosa; 10.05%). In addition, at lower percentages, we isolated glucose-non-fermenting, Gram-negative bacteria and other Enterobacteriaceae. The antibiotic resistance varied greatly between species; the most resistant were the non-fermenting Gram-negative rods. E. coli exhibited lower resistance to third generation cephalosporins, quinolones and carbapenems. By contrast, Klebsiella was resistant to many cephalosporins and penicillins, and to a certain extent to carbapenems due to carbapenemase production. The non-fermenting bacteria were highly resistant to antibiotics, but were generally sensitive to colistin. S. aureus was resistant to ceftriaxone (100%), penicillin (91.36%), amoxicillin/clavulanate (87.50%), amikacin (80.00%) and was sensitive to levofloxacin, doxycycline, gentamycin, tigecycline and teicoplanin. The Enterobacteriaceae resistance was only slightly higher in the ICU, particularly to carbapenems (imipenem, 31.20% in the ICU vs. 14.30% in the surgical wards; risk ratio = 2.182). As regards Staphylococcus species, but for non-fermenting bacteria, even if the median was almost the same, the antibiotic resistance index values were confined to the upper limit in the ICU. The data gathered from this study may help infection control teams to establish effective guidelines for antibiotic therapies in various surgical procedures, in order to minimize the risk of developing SSIs by the efficient application of the anti-infection armamentarium.

show abstract

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance development following surgical site infections

Călina

Docea

Rosu

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

show abstract

“…Resistance or antibiotic sensitivity of the isolated strains was tested on Mueller-Hinton agar (MHA, oxoid) by the standard single disk method as described previously (Bauer, Kirby, Sherris, & Turck, 1966) and recently (Kassim, Omuse, Premji, & Revathi, 2016). Antibiograms of the isolates were critically examined.…”

Section: Antibiotic Sensitivity Testingmentioning

confidence: 99%

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

Samad

Abbas

Ahmad

et al. 2018

Journal of Food Safety

View full text Add to dashboard Cite

The objective of this study was to conduct a qualitative analysis of raw beef meat sold in the city of Quetta, Pakistan for presence and drug sensitivity of the potentially pathogenic Escherichia coli strain O157:H7. The study used 200 raw beef meat samples collected from retail butcher shops. Conventional and rapid biochemical tests, latex agglutination and multiplex polymerase chain reaction (PCR) using primers designed for the rfb O157 and flic H7 genes were used to detect E. coli O157:H7. All O157:H7 isolates were also tested for Shiga toxin genes stx 1 and stx 2. The prevalence of E. coli O157:H7 in collected beef meat samples was 10%. Detection through PCR was found more sensitive than detection of O and H antigens. The quantity of E. coli O157:H7 isolates positive for Shiga toxins was 50% (20% for stx 1, 45% for stx 2 and 10% for both stx 1 and stx 2). Season wise variation showed highest E. coli O157:H7 prevalence during summer months. A further concern is that E. coli O157:H7 isolates were resistant to a range of common antibiotics. The results indicate an urgent need for applying proper food hygiene practices in the Quetta region to reduce incidence of foodborne diseases and they also emphasize the global problem of antimicrobial resistance. Practical applications E. coli O157:H7 is as a potentially threatening foodborne pathogen. A significant prevalence of E. coli O157:H7 detected in raw beef meat from retail outlets in the city of Quetta indicates an urgent need for applying proper food hygiene practices in the Quetta region to reduce the incidence of foodborne diseases. Furthermore, resistance of the E. coli O157:H7 isolates to a range of commonly used antibiotics emphasizes the global problem of antimicrobial resistance. The multiplex PCR method used here is a reliable, sensitive, and relatively rapid technique for detecting E. coli O157:H7 in food and environmental samples and important for ongoing surveillance to minimize contamination of raw meat products and associated cross contamination by E. coli O157:H7.

show abstract

“…The degree of sensitivity was determined by measuring the zone of growth inhibition produced by the diffusion of the antibiotic into the surrounding medium after the incubation at 37 °C for 24 h under aerobic conditions. The results were interpreted according to the methods of Kassim et al (2016) and Sahu et al (2018).…”

Section: Antibacterial Sensitivity Testmentioning

confidence: 99%

Bacteriological, Hematological and Biochemical Diagnostic Studies on Diarrheic Arabian Horse Foals Caused by Enterobacterial Infections

Ata¹,

Nasr²,

Mohamed³

et al. 2020

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

F oal diarrhea is a worldwide major problem in equines and is reported to be the most common cause of death in young age (Barr, 2018; Haq et al., 2018). The affected animals show diarrhea, inappetance, and poor body condition as major clinical signs. While in some per-acute cases, high fever, leukopenia, increase heart rate, anorexia and septic shock were detected (Haq et al., 2017a). It is recorded that nearly 80% of the foals suffered from diarrhea at least one time during their life (Frederick et al., 2009). A single causative agent is often not found (Schoster et al., 2015). However, coinfection between infectious agents was recorded as being more prevalent and may result in

show abstract

Comparison of Clinical Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing guidelines for the interpretation of antibiotic susceptibility at a University teaching hospital in Nairobi, Kenya: a cross-sectional study

Cited by 105 publications

References 9 publications

Antimicrobial resistance development following surgical site infections

Antimicrobial resistance development following surgical site infections

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

Bacteriological, Hematological and Biochemical Diagnostic Studies on Diarrheic Arabian Horse Foals Caused by Enterobacterial Infections

Contact Info

Product

Resources

About