Comparison of commonly used ICR stocks and the characterization of Korl:ICR

Abstract

Mouse is a commonly used animal in life science studies and is classified as outbred if genetically diverse and inbred if genetically homogeneous. Outbred mouse stocks, are used in toxicology, oncology, infection and pharmacology research. The National Institute of Food and Drug Safety Evaluation (NIFDS; former the Korea National Institute of Health) have bred ICR mice for more than 50 years. We investigated to provide users with information and promote accountability to the Korl:ICR. To secure the indigenous data, biological characteristics of Korl:ICR were identified by comparing with other ICR stocks. This domestic ICR stock was denominated as ‘Korl:ICR’. Phylogenetic analysis using SNPs indicated that the population stratification of the Korl:ICR was allocated different area with other ICR. In addition, we measured litter size, body weight, body length, various organ weight, hematology and clinical blood chemistry of the Korl:ICR compared to other ICR. Otherwise, there are no significant differences among the biological phenotypes of Korl:ICR and other ICR. These results suggest that as a genetically indigenous source colony, the Korl:ICR is seperated (or independent) stock with other ICR. Also, we confirmed that there is no difference among the Korl:ICR and other ICR on biological phenotypes. Therefore, the Korl:ICR source colony might be a new stock in distinction from other ICR, it is a good milestone in securing ownership of the national laboratory animal resource. The NIFDS expects that the Korl:ICR mice will be useful animal resource for our domestic researchers.

Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources

“…These changes in the parameter values are considered be due to the administration of BoNT/A muscle injection. However, many of the changed clinical pathologic parameter values were found to be within the normal range of reference to the previous studies [25][26][27]. Given that this is the first report of clinical pathology data after intramuscular administration of BoNT/A in ICR mice, our findings are meaningful and justify further experiments.…”

High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

“…Surprisingly, in contrast with the in vitro results, in vivo embryo development was enhanced in 6-month-old mice treated with high frequency of human ASC-CM IV. The mean numbers of implanted fetuses was increased two-fold compared to the control group, which is even higher than the average litter size of young commercial ICR mice [36]. The litter size is an intricate physiological element affected by sequential events including ovulation, fertilization, embryo development, and fetal survival [37].…”

Therapeutic potential of neurogenesis and melatonin regulation in Alzheimer's disease

“…4 In AD mouse models, melatonin supplementation was found to ameliorate mitochondrial damage and downregulate the expression of key harmful proteins, leading to a significant improvement of cognitive function. [14][15][16] The antioxidative properties of melatonin were reported to prevent the overproduction of damaging free radicals generated from pathological processes in AD and reduce the subsequent neuronal damage that would otherwise have occurred. 17 Several preclinical studies have also indicated that melatonin may play a role in adult neurogenesis (see Tables 1 and 2).…”