Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

Valbuena, Diana; Póo, Maria E.; Aguilar-Gallardo, Cristóbal; Martínez, Sebastián; Cobo, Ana; Pellicer, António; Simón, Carlos

doi:10.1016/j.fertnstert.2011.10.008

Cited by 15 publications

(9 citation statements)

References 42 publications

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“…Most ultra-rapid cooling vitrification techniques require vitrification containers such as electron microscope grids [33], open pulled straws [20,35], CryoLoop [17,18], CryoTech [7], Cryotops [36,38], cryotip [36], Cryotube [21,22], or thin plastic strips [29]. These tools have improved the development of vitrification and led to the successful production of offspring from vitrified and warmed embryos [6,8].…”

Section: Introductionmentioning

confidence: 99%

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Chang

et al. 2015

Cryobiology

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Section: Introductionmentioning

confidence: 99%

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Chang

et al. 2015

Cryobiology

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“…Many studies have been carried out in order to create a vitrification protocol that will be as harmless as possible for oocytes and embryos [4,5,[8][9][10][11][12]. In this work, we designed and tested a new protocol with a short time of exposure in vitrification and thawing solutions in order to reduce the danger of toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…The introduction of vitrification into IVF came along with an enhancement of the relevant research. As a result, new vitrification protocols and devices have appeared aiming to improve further the success rates of cryo cycles [8][9][10][11].…”

mentioning

confidence: 99%

Evaluation of a New Vitrification Protocol for Oocyte Cryopreservation

Παναγοπούλου¹,

Charalabopoulos²,

Papachristou³

et al. 2013

Human Genet Embryol

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During the last years, vitrification was introduced into clinical practice. Although vitrification counts several years of experimental studies, this method is still under development. In this study, a new vitrification protocol was evaluated. Materials and methods: An experimental study conducted in a University laboratory evaluated a new, short vitrification protocol including two vitrification and two thawing solutions, made in-house. The vitrification solutions contained dimethyl sulfoxide, ethylenoglycol and human serum albumin. The thawing solutions contained sucrose and human serum albumin. Rat oocytes, allocated in two groups, were used. In the control group, the oocytes were vitrified using a standard protocol. In the experimental group, the oocytes were vitrified using the new protocol. The endpoint was the survival rate of the oocytes just after thawing and 48 hours later. Results: The survival rate just after thawing was similar in the two groups (62.5% for the control group and 60% for the experimental group, p=0.18). However after 48 hour incubation, there was a significant difference between the two groups: 55% survival for the control group and 45% for the experimental group (p=0.008). Conclusion: The two vitrification protocols achieved similar survival rates just after thawing. However, after 48 hours incubation of thawed oocytes, the standard method had higher survival rates, than the in-house one.

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“…Vitrification of mouse embryos has been performed in a variety of vitrification containers using the EM grid [33,34], cryotop [22,35,36], cryoloop [15], and OPS [10-12] methods. In this study, to develop a simple, rapid, cost-effective, and successful material for simultaneous vitrification, we investigated the effects of cleavage-stage mouse embryos and blastocysts during vitrification using several types of containers.…”

Section: Discussionmentioning

confidence: 99%

Vitrification of mouse embryos using the thin plastic strip method

Ryu

Hur

Ann

et al. 2012

Clin Exp Reprod Med

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ObjectiveThe aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates.MethodsMouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in EM grid, cryotop, and TPS containers by vitrification in 15% ethylene glycol, 15% dimethylsulfoxide, 10 µg/mL Ficoll, and 0.65 M sucrose, and 20% serum substitute supplement (SSS) with basal medium, respectively. For the three groups in which the embryos were thawed in the EM grid, cryotop, and TPS containers, the thawing solution consisted of 0.25 M sucrose, 0.125 M sucrose, and 20% SSS with basal medium, respectively. Rates of survival, re-expansion, reaching the hatched stage, and apoptosis after thawing were compared among the three groups.ResultsDevelopmental competence after thawing of vitrified expanded and hatching-stage blastocysts using cryotop and TPS methods were significantly higher than survival using the EM grid (p<0.05). Also, apoptosis positive nuclei rates after thawing of vitrified expanded blastocysts using cryotop and TPS were significantly lower than when using the EM grid (p<0.05).ConclusionThe TPS vitrification method has the advantages of achieving a high developmental ability and effective preservation.

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Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

Cited by 15 publications

References 42 publications

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)

Evaluation of a New Vitrification Protocol for Oocyte Cryopreservation

Vitrification of mouse embryos using the thin plastic strip method

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