Comparison of CTAB Method and Wizard Genomic DNA Purification System Kit from Promega on DNA Isolation of Local Varities of Rice of South Sumatera

Hanum, Laila; Windusari, Yuanita; Setiawan, Arum; Muharni, Muharni; Adriansyah, Fickri; Mubarok, Amin Ali

doi:10.26554/sti.2018.3.1.26-29

Cited by 11 publications

(9 citation statements)

References 2 publications

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“…However, the low DNA concentration of cosmetic showed by UV/VIS spectrophotometer (3.252-4.222 ng/ µL) satisfyingly to perform PCR assay. Conversely, compared to the previous study by Hanum et al (2018), the isolation of DNA from unprocessed food and processed food gave significantly high DNA yields and purity of DNA, however, when it was applied to the cream-based product, the purity of DNA and DNA yields was lower. This may be due to the over-processing of the sample or low extraction efficiency for a collagen-based product which is generally present in the form of protein.…”

Section: Optimization Of Dna Extraction Using Wizard Genomic Dna Puricontrasting

confidence: 71%

“…It has been reported that isolation of DNA from rice and corn by using Wizard Genomic DNA Purification System kit from Promega is more efficient, high DNA yield and purity when compared with CTAB method (Hanum et al, 2018). However, the most suitable extraction kit for the isolation of porcine DNA from cosmetics is Power PrepTM DNA when compared with CTAB method, Nucleo spin food kit, QIAamp DNA stool mini kit, TIANamp Genomic DNA kit, and Wizard Genomic DNA purification kit (Kim et al, 2018).…”

Section: Optimization Of Dna Extraction Using Wizard Genomic Dna Purimentioning

confidence: 99%

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Screening porcine DNA in collagen cream cosmetic products

Zabidi¹,

Fauzi²,

Razak³

et al. 2020

Food Res.

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Pig derivatives such as collagen are commonly added as an ingredient in cosmetics to improve appearance and skin health. To ensure cosmeceutical products comply with halal regulations in Muslim countries, the development of a quick, valid, practical, and economical method to detect the presence of porcine DNA is necessary. The aim of this study was to detect the presence of pork DNA from cosmetic product. Genomics DNA from highly processed cosmetics cream products and raw meat (as positive control) were isolated by using Wizard Genomic DNA purification kit from Promega. Five cosmetics cream samples that labeled as collagen cream were purchased through the online store. One of the products is declared contains piggy collagens, one is halal and other three are unknown source. Polymerase chain reaction (PCR) assay was performed to amplify the fragment of the 12S rRNA gene by a set of species-specific primer which produces amplicons length 387 bp in porcine DNA. The result showed the presence of porcine DNA which was isolated from raw pork, cream cosmetics that contain piggy collagens and cream hands that contains collagen from unknown source using commercially PCR MyTaq™ DNA polymerase kit and a set of species-specific primer with an annealing temperature of 44.4 ºC. The band produced from this PCR was the highest intensity. The success of the amplification of porcine DNA shows that this method is practical, easy and efficient for routine product analysis for halal authentication in undeclared and declared of the porcine material presence in the product. Hence, consuming cosmetic cream contains porcine DNA is prohibited according to the Islamic view in Malaysia.

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Section: Optimization Of Dna Extraction Using Wizard Genomic Dna Puricontrasting

confidence: 71%

Section: Optimization Of Dna Extraction Using Wizard Genomic Dna Purimentioning

confidence: 99%

Screening porcine DNA in collagen cream cosmetic products

Zabidi¹,

Fauzi²,

Razak³

et al. 2020

Food Res.

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“…Extraction of bacterial DNA was conducted using the Wizard Genomic DNA Purification Kit (PROMEGA, USA) following the manufacturer's protocols as described in a published procedure [6] . The cells were pelletized by centrifuging 1 ml of overnight culture in a 1.5 ml microcentrifuge tube at 13,000 × g for 2 min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Dataset of 16S ribosomal DNA sequences of bacteria isolated from marine red algae Kappaphycus alvarezii

et al. 2022

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The data provided in the article contains bacterial community profiles present on the surface of red algae ( Kappaphycus alvarezii ) isolated directly after collection and after 30 days of cultivation in a closed circulation system. The explants of Kappaphycus alvarezii were cultivated in a laboratory setting under controlled growth conditions for 30 days in order to determine bacteria that could adapt to controlled culture conditions. Amplification and sequencing of bacterial 16S rDNA amplicon were performed on bacterial isolates associated with the seedlings. The 16S rDNA gene sequences were analyzed, trimmed, and assembled into contigs using DNA Baser Sequence Assembler (V5) software. Taxonomic identification for the assembled sequences was achieved using the online BLAST (blastn) algorithm, and the construction of a phylogenetic tree was performed using the MEGA7 software. The data reveals a distinct set of microbial variations between day one and day 30. The phylogenetic tree depicts four major clusters, Vibrio, Pseudoalteromonas, Alteromonas , and Bacterioplanes resident on the surface of the K. alvarezii . Comparison between these two bacterial groups provides evidence of the persistent marine bacteria that adapt to the long-term culture in closed circulation systems. Raw data files are available at the GenBank, NCBI database under the accession number of MZ570560 to MZ570580.

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“…The supernatant was discarded and washed with 70% ethanol (1 vol). After washing the purified DNA was vacuum dried for 15–20 min and dissolved in 100 µl of TE buffer [17] . Prior to dissolution the TE buffer was pre-warmed at 65 °C.…”

Section: Protocol Iv-wizard Genomic Dna Purification Kit (Promega)mentioning

confidence: 99%

Comparison and optimization of DNA Isolation protocols for high throughput genomic studies of Acacia pachyceras Schwartz

et al. 2022

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Comparison of CTAB Method and Wizard Genomic DNA Purification System Kit from Promega on DNA Isolation of Local Varities of Rice of South Sumatera

Cited by 11 publications

References 2 publications

Screening porcine DNA in collagen cream cosmetic products

Screening porcine DNA in collagen cream cosmetic products

Dataset of 16S ribosomal DNA sequences of bacteria isolated from marine red algae Kappaphycus alvarezii

Comparison and optimization of DNA Isolation protocols for high throughput genomic studies of Acacia pachyceras Schwartz

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