Comparison of Culture and PCR Methods in Detection of Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis in Children with Otitis Media with Effusion

Abstract

A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : The etiology and pathogenesis of otitis media with effusion (OME) is still unclear despite many studies within the last four decades. Polymerase chain reaction (PCR) based procedures are suggested for detection of the causative bacteria supposed to inflict multiple infections. In the current study, culture and PCR based approaches were used to detect the frequency of Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, which have been known as common pathogens in middle ear effusions (MEE) of patients with otitis media. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : The DNAs of these three bacteria were detected by standard and multiplex PCR techniques in MEE specimens and their diagnostic values were evaluated in comparison to the conventional culture method. R Re es su ul lt ts s: : Samples from 67 OME suspected children were analysed retrospectively. Two H. influenzae and two M. catarrhalis isolates were recovered by conventional culture method (6.0%; 4/67). Out of the 67 samples, seven S. pneumoniae, nine H. influenzae, and eleven M. catarrhalis isolates were detected vith PCR. In five samples, two concurrent bacteria were detected in following combinations: two S. pneumoniae and H. influenzae, two S. pneumoniae and M. catarrhalis, and one H. influenzae and M. catarrhalis. Sensitivity, specificity, positive predictive value and negative predictive value rates of the PCR technique were 100.0%, 71.4%, 18.2% and 100.0%, respectively. The difference between culture and PCR was statistically significant (p<0.001). C Co on nc cl lu us si io on n: : Although the specificity and positive predictive values are low, PCR, which allows rapid screening is feasible for detecting the most common fastidious bacteria that lead to OME. K Ke ey y W Wo or rd ds s: : Otitis media with effusion; streptococcus pneumoniae; haemophilus influenzae; moraxella catarrhalis; polymerase chain reaction; culture Ö ÖZ ZE ET T A Am ma aç ç: : Efüzyonlu orta kulak iltihabının (EOKİ) etiyolojisi ve patogenezi, son 40 yıl boyunca yapılan çok sayıda çalışmaya rağmen hala çok açık değildir. Çoklu enfeksiyöz ajanların sorumlu olduğu düşünülen bu hastalıkların nedenini saptamak için polimeraz zincir reaksiyonu (PZR) temeline dayanan yöntemler önerilmektedir. Bu çalışmada, EOKİ şüpheli çocuklardan alınmış 67 orta kulak sıvısı (OKS) örneği incelendi. OKS'de yaygın patojenler olarak bilinen H. influenzae, S. pneumoniae ve M. catarrhalis oranlarını saptamak için kültür ve PZR teknikleri uygulandı. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu üç bakterinin DNA'ları OKS örnek-lerinde standart ve multipleks PZR yöntemleri ile saptandı ve bunların tanısal değerleri, altın standart olarak kabul edilen konvansiyonel kültür yöntemi ile karşılaştırmalı olarak değerlendirildi. B Bu ul lg gu ul la ar r: : Konvansiyonel kültür yöntemiyle iki H. influenzae ve iki M. catarrhalis kökeni izole edildi (%6,0, 4/67). PZR ile 7 S. pneumoniae, 9 H. influenzae ve 11 M. ca...

Enhancing ICU care through NGS – based identification of infectious agents : A comparative study

“…Use of culture based diagnosis is especially prevalent in low-income countries due to the low cost, quick turn around time of culture testing. However, there are drawbacks to relying solely on this test (speci cally where unusual or slow growing microbes are present) and the nature of the test may result in preferential enhancement of speci c organisms which can impact clinical speci city [19].…”