Comparison of different methods aiming to account for/overcome matrix effects in LC/ESI/MS on the example of pesticide analyses

Kruve, Anneli; Leito, Ivo

doi:10.1039/c3ay26551j

Cited by 44 publications

(16 citation statements)

References 40 publications

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“…Some of the common methods that account for overcoming matrix effects in LC–ESI‐MS (matrix‐matched calibration, standard addition, etc.) were compared . The relationship between the matrix effects and physicochemical properties was investigated, and it indicated that the molecular polarity and volume of the analyte increase the matrix effect, while hydrophobicity and an increasing number of nonpolar carbon atoms in the analyte decrease the matrix effects .…”

Section: Resultsmentioning

confidence: 99%

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

Yan

Liu

et al. 2014

J of Separation Science

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This paper describes a novel, rapid, and sensitive analytical method for monitoring four triazolone herbicides in cereals (wheat, rice, corn, and soybean), using a quick, easy, cheap, effective, rugged, and safe sample extraction procedure followed by ultrahigh performance liquid chromatography coupled with tandem mass spectrometry. The four triazolone herbicides (amicarbazone, carfentrazone-ethyl, sulfentrazone, and thiencarbazone-methyl) were extracted using acidified acetonitrile (containing 1% v/v formic acid) and subsequently purified with octadecylsilane (C18 ) prior to sample analysis. Ultrahigh performance liquid chromatography coupled with tandem mass spectrometry was operated in positive and negative ionization switching mode. Amicarbazone and carfentrazone-ethyl were detected in the positive mode (ESI+), while sulfentrazone and thiencarbazone-methyl were detected in the negative mode (ESI-). All compounds were successfully separated in less than 3.0 min. Further optimization achieved desired recoveries ranging from 74.5 to 102.1% for all analytes with relative standard deviation values ≤17.2% in all tested matrices at three levels (10, 100, and 500 μg/kg). The limits of detection for all compounds were ≤2.3 μg/kg, and the limits of quantitation did not exceed 7.1 μg/kg. The developed method showed excellent linearity (R(2) ≥ 0.994) and was proven to be highly efficient and reliable for the routine monitoring of triazolone herbicides in cereals.

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Section: Resultsmentioning

confidence: 99%

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

Yan

Liu

et al. 2014

J of Separation Science

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“…According to the experiment performed, the matrix factor value obtained for the QCL was 101.376%, meaning that at this concentration, there was ions enhancement, although not significantly, the value was near 100% [10][11][12][13]. The matrix factor value obtained in the high concentration was 85.053%, meaning that there was ions suppression [14,15].…”

Section: Selectivity Testmentioning

confidence: 95%

Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

Harahap

Maysyarah

2017

Int J App Pharm

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Objective: This study developed a sensitive, selective, and valid method for analyzing clopidogrel in human plasma using liquid chromatographytandem mass spectrometry (LC-MS/MS). Methods:The chromatography separation was performed on the waters ACQUITY UPLC Class BEH C 18 1.7 µm (2.1 × 100 mm) column, consisting of 0.1% formic acid in water and 0.1% formic acid in acetonitrile (30-70) as the mobile phase, and an isocratic elution with a flow rate of 0.2 mL/minutes. The mass detection was performed using a Waters Xevo TQD equipped with positive electrospray ionization in the multiple reaction monitoring modes. The clopidogrel was detected at m/z 322.086>212.097 and irbesartan as an internal standard at m/z 429.233>207.131. The sample was prepared with protein precipitation method using acetonitrile, vortex mixed for 10 minutes, and centrifuged at 13,000 rpm for 20 minutes.Results: This method showed a linear result at the concentration range of 0.2-10 ng/ml with r≥0.9997. Conclusions:The developed method provides sensitivity, linearity, precision, and accuracy and is suitable for analysis of clopidogrel in human plasma using LC-MS/MS.

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“…One reason for the small matrix effect can be the low flow rate in this method, which can reduce matrix effects in ESI [51]. This can be further improved by post-column splitting [58] or using smaller dimensions of the column with proportionally lower flow rate. Moreover, using other ionization sources such as APCI [59] and APPI [60] might also help to reduce the matrix effect as they are less prone to interferences.…”

Section: Methods Validationmentioning

confidence: 99%

Development of an LC-MS method for determination of nitrogen-containing heterocycles using mixed-mode liquid chromatography

2020

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N-containing heterocycles (NCHs) are largely used as precursors for pharmaceuticals and can enter the environment. Some NCHs have been shown to be toxic, persistent, and very mobile in the environment. Thus, they have received increasing attention in the past years. However, the analysis of these polar compounds in environmental samples is still a challenge for liquid chromatography. This paper investigates the use of mixed-mode liquid chromatography (MMLC), which has reversed-phase and ion exchange characteristics for measurements of NCHs in water. NCHs with low pK a (i.e., < 2.5) display mainly reversedphase interactions (neutral species) with the stationary phase and those with higher pK a (i.e., > 5) interact by a mixture of reversed-phase/ion exchange/HILIC mechanism. It was also shown that the presented method performs well in the quantification of the majority of the selected NCHs in surface water with MDLs between 3 and 6 μg/L, a low matrix effect and recoveries in the range of 77-96% except for pyridazine exhibiting 32% were achieved. The method was successfully employed to follow the degradation of NCHs in ozonation. Keywords Nitrogen-containing heterocycles. Liquid chromatography. Reversed-phase. Cation exchange. Mixed-mode chromatography Published in the topical collection Persistent and Mobile Organic Compounds-An Environmental Challenge with guest editors Torsten C.

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Comparison of different methods aiming to account for/overcome matrix effects in LC/ESI/MS on the example of pesticide analyses

Cited by 44 publications

References 40 publications

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

Development of an LC-MS method for determination of nitrogen-containing heterocycles using mixed-mode liquid chromatography

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