Comparison of different methods of measuring 8-oxoguanine as a marker of oxidative DNA damage

Collins, Andrew; Brown, J.; Bogdanov, M.; Cadet, Jean Paul; Cooke, Marcus S.; Douki, Thierry; Dunster, C.; Eakins, J.; Epe, Bernd; Evans, Mark D.; Farmer, P.; Gedik, Catherine M.; Halliwell, Barry; Herbert, K.; Hofer, Tim; Hutchinson, R.; Jenner, A.; Jones, Geoffrey; Kasai, Hiroshi; Kelly, F.; Lloret, A.; Loft, Steffen; Lunec, Joseph; Mcewan, M.; Möller, Lennart; Oliński, Ryszard; Podmore, I.; Poulsen, Henrik Enghusen; Ravanat, J. L.; Rees, J. F.; Reetz, F.; Shertzer, Howard G.; Spiegelhalder, B.; Turesky, R.; Tyrrell, R.; Vina, J.; Vinicombe, D.; Weimann, Allan; Wergifosse, B. De; Wood, Sharon

doi:10.1080/10715760000300331

Cited by 122 publications

(22 citation statements)

References 4 publications

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“…Although the other nucleobases of DNA react with OH − in a similar manner, lesions associated with 8-oxo-dG are the most abundant DNA lesions because they form easily; 8-oxo-dG is promutagenic and is therefore a potential biomarker of carcinogenesis [82]. 8-OH-dG level can be measured in animal organs and in human samples such as in the urine, organs, and leukocyte DNA and serves as a biomarker of oxidative stress, aging, and carcinogenesis [83, 84]. …”

Section: Biomarkers Of Oxidative Stress and Nucleic Acid Oxidationmentioning

confidence: 99%

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Sung

Hsu

Chen

et al. 2013

Oxidative Medicine and Cellular Longevity

162

110

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Patients with chronic kidney disease (CKD) have high cardiovascular mortality and morbidity and a high risk for developing malignancy. Excessive oxidative stress is thought to play a major role in elevating these risks by increasing oxidative nucleic acid damage. Oxidative stress results from an imbalance between reactive oxygen/nitrogen species (RONS) production and antioxidant defense mechanisms and can cause vascular and tissue injuries as well as nucleic acid damage in CKD patients. The increased production of RONS, impaired nonenzymatic or enzymatic antioxidant defense mechanisms, and other risk factors including gene polymorphisms, uremic toxins (indoxyl sulfate), deficiency of arylesterase/paraoxonase, hyperhomocysteinemia, dialysis-associated membrane bioincompatibility, and endotoxin in patients with CKD can inhibit normal cell function by damaging cell lipids, arachidonic acid derivatives, carbohydrates, proteins, amino acids, and nucleic acids. Several clinical biomarkers and techniques have been used to detect the antioxidant status and oxidative stress/oxidative nucleic acid damage associated with long-term complications such as inflammation, atherosclerosis, amyloidosis, and malignancy in CKD patients. Antioxidant therapies have been studied to reduce the oxidative stress and nucleic acid oxidation in patients with CKD, including alpha-tocopherol, N-acetylcysteine, ascorbic acid, glutathione, folic acid, bardoxolone methyl, angiotensin-converting enzyme inhibitor, and providing better dialysis strategies. This paper provides an overview of radical production, antioxidant defence, pathogenesis and biomarkers of oxidative stress in patients with CKD, and possible antioxidant therapies.

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Section: Biomarkers Of Oxidative Stress and Nucleic Acid Oxidationmentioning

confidence: 99%

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Sung

Hsu

Chen

et al. 2013

Oxidative Medicine and Cellular Longevity

162

110

View full text Add to dashboard Cite

show abstract

“…The Programme's more recent focus on interlaboratory sample exchange and the contribution of many of the contractors to the work of the ESCODD project, has greatly improved the reliability and reproducibility of the data. ESCODD, which involves 27 laboratories, was established in 1997 after a need for the development of a quality control programme, associated with the measurement of 8‐oxo‐dG was identified (Collins et al . 2000; http://www.rowett.ac.uk/escodd).…”

Section: Oxidised Dna‐base Damagementioning

confidence: 99%

Antioxidants in food: a summary of the review conducted for the Food Standards Agency

et al. 2002

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The British Nutrition Foundation recently completed a critical review of the Food Standards Agency's Antioxidants in Food program. The projects funded as part of this program, which addressed biomarkers of oxidative damage, gene expression, supplementation with dietary antioxidants and bioavailability of dietary antioxidants, were evaluated and set within an international context. This paper summarises the findings and recommendations of this review. Although there is a substantial body of evidence that a diet rich in plant foods (particularly fruit and vegetables) conveys health benefits, intervention trials have been unable to demonstrate any causal link between antioxidant supplementation and chronic disease. Difficulties in undertaking such trials with long‐term disease endpoints, as is the case with cancer and cardiovascular disease, has led to considerable interest in the identification of robust biomarkers of oxidative damage. However, the relevance of these biomarkers to disease risk remains an issue and little is known about what might be regarded as a normal level of endogenous oxidative damage. The review concluded that the research program should focus on a wider scope of potential mechanisms, rather than antioxidant mechanisms alone. It also recommended that more research is needed on the bioavailability and tissue distribution of antioxidants and the impact of factors such as genotype, age and illness before embarking on a multicentre intervention trial.

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“…Therefore, the 8-OHdG levels are considered to be a valid marker of oxidative DNA damage. In addition, new techniques have been introduced into this field as gas chromatography with mass spectrometry [17,25], electrospray ionization mass spectrometry (MS) [26], liquid chromatography with mass spectrometry [27][28][29][30] and capillary electrophoresis [31][32][33][34][35][36]. From the direct ones, high performance liquid chromatography (HPLC) with electrochemical detection (ED) [23,24] belongs to the most commonly used method for determination of 8-OHdG.…”

Section: Determination Of 8-ohdgmentioning

confidence: 99%

Electrochemical Study of DNA Damaged by Oxidation Stress

Zítka¹,

Křížková²,

Skaličková³

et al. 2013

Combinatorial Chemistry & High Throughput Screening

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Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed. The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.

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Comparison of different methods of measuring 8-oxoguanine as a marker of oxidative DNA damage

Cited by 122 publications

References 4 publications

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Antioxidants in food: a summary of the review conducted for the Food Standards Agency

Electrochemical Study of DNA Damaged by Oxidation Stress

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