Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of <i>C. jejuni</i> and <i>C. coli</i> from Broiler Flocks at Primary Production

Vidal, Ana Maria Centola; Rodgers, John; Arnold, Mark; Clifton-Hadley, F. A.

doi:10.1111/zph.12009

Cited by 22 publications

(32 citation statements)

References 50 publications

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“…However, there is not yet an accepted standard method for the detection and isolation of Campylobacter spp. at farm level (Vidal et al, 2013). In commercial broiler chickens, Campylobacter spp.…”

Section: Resultsmentioning

confidence: 99%

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

2016

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Campylobacteriosis and salmonellosis are the most frequently reported zoonoses and among the most common causes of diarrhoeal illness in the European Union and the United States, and their incidence appears to be increasing. Campylobacter species are routinely found in poultry, swine, cattle, dairy cows and sheep. So far, there are few descriptions of Campylobacter isolation from rabbits. Rabbit meat is a common item in the Mediterranean diet. In this context, the aim of the present study was to investigate the occurrence of Campylobacter spp. in healthy rabbits reared in intensive farms in the Alto Palancia region, eastern Spain. Caecal contents from 70 healthy does reared on 7 different farms were collected. Bacteriological culture was performed in accordance with ISO 10272-1:2006. All samples tested negative for Campylobacter spp. To our knowledge, this is the first study in which comprehensive monitoring was specifically carried out in order to provide data on the occurrence of thermophilic Campylobacter spp. in large intensive rabbit farms in Spain. However, further microbiological studies throughout the Spanish territory are needed to determine the prevalence and risk of other foodborne pathogens in rabbits at farm level.

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Section: Resultsmentioning

confidence: 99%

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

2016

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“…The use of boot swabs moistened in transport media can be an efficient alternative to caecal or faecal sampling at the farm (Vidal et al, 2013). This kind of sample has shown a good sensitivity and can be a good method for an early PCR detection of a Campylobacterpositive flock, for monitoring purposes.…”

Section: Resultsmentioning

confidence: 99%

“…This includes gaining insight into the dynamics of infection in broiler farms. There are few studies that have compared the suitability of different types of sample for the detection of Campylobacter at the slaughterhouse (Ugarte-Ruiz et al, 2012), or the performance of sampling methods on the farm (Vidal et al, 2013). As a first step to conduct studies of Campylobacter dynamics at farm level, we evaluated the sensitivity of two types of sample (caecal contents and cloacal swabs) in order to choose the most suitable one for the early detection of Campylobacter when culture methods are used.…”

Section: Introductionmentioning

confidence: 99%

Assessment of two different types of sample for the early detection and isolation of thermophilicCampylobacterin broiler farms

2015

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In order to assess the optimal method for the early detection and isolation of thermophilic Campylobacter in broilers at farm level, two types of samples were compared: caecal contents obtained by necropsy and cloacal swabs transported in charcoal Amies medium. The study was conducted in five batches of broilers from five different farms, where weekly samples (caecal contents and cloacal swabs) from 30 birds were obtained. Samples were plated onto selective agar (modified charcoal cefoperazone desoxycholate agar, mCCDA) for Campylobacter isolation. Four out of five batches were positive for Campylobacter. No marked differences in sensitivity of both sample types were observed. However, a higher percentage of positive birds were detected when cloacal swabs were used. The results show that cloacal swab samples are adequate, and in some cases even better than caecal samples for the early detection of Campylobacter in broiler flocks at farm level. Also, this sample avoids sacrificing birds to test Campylobacter, which not only allows saving time in sample collection, transportation and processing at the laboratory, but also improves bird welfare and cost of sampling.

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“…These authors conclude that the enrichment method used affects both the number and species of Campylobacter isolated from naturally contaminated samples. In a separate study, Vidal et al (2013) found that enrichment in Exeter broth significantly increased the sensitivity of swab and cecal samples for the detection of Campylobacter spp. in broiler flocks.…”

Section: Isolation Enrichment and Maintenancementioning

confidence: 96%

The Family Campylobacteraceae

Lastovica¹,

On²,

Zhang³

2014

The Prokaryotes

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Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Cited by 22 publications

References 50 publications

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

Assessment of two different types of sample for the early detection and isolation of thermophilicCampylobacterin broiler farms

The Family Campylobacteraceae

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