Comparison of DNA isolation methods for detection of foodborne pathogens by real-time PCR from foods

구은정,; 김동호,; 오세욱,

doi:10.9721/kjfst.2016.48.4.335

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Salmonella was initially screened by biochemical assays, namely indole, sugar fermentation assay, production of urease and H 2 S, and production of lysine and ornithine decarboxylase. Genomic DNA of Salmonella species was extracted by the boiling method described by Coo et al [ 26 ] to be used as template DNA for PCR. The isolates were identified as Salmonella by PCR detection of the specific gene inv A, as previously described [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation, antimicrobial resistance and virulence characterization of Salmonella spp. from fresh foods in retail markets in Hangzhou, China

Qian,

Xu,

Wang

et al. 2023

PLoS ONE

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Salmonella can cause severe foodborne diseases. This study investigated the prevalence of Salmonella spp. in fresh foods in Hangzhou market and their harborage of antibiotic resistance and virulence genes, antibiotic susceptibility, and pathogenicity. A total of 500 samples (pork, n = 140; chicken, n = 128; vegetable, n = 232) were collected over a one-year period. Salmonella was found in 4.2% (21) of samples with the detection rate in pork, chicken and vegetables as 4.3% (6), 6.3% (8), and 3% (7), respectively. One Salmonella strain was recovered from each positive sample. The isolates were identified as six serotypes, of which S. Enteritidis (n = 7) and S. Typhimurium (n = 6) were the most predominant serotypes. The majority of isolates showed resistance to tetracycline (85.7%) and/or ciprofloxacin (71.4%). Tetracycline resistance genes showed the highest prevalence (90.5%). The occurrence of resistance genes for β-lactams (blaTEM-1, 66.7%; and blaSHV, 9.5%) and aminoglycosides (aadA1, 47.6%; Aac(3)-Ia, 19%) was higher than sulfonamides (sul1, 42.9%) and quinolones (parC, 38.1%). The virulence gene fimA was detected in 57.1% of isolates. Gene co-occurrence analysis implied that resistance genes were associated with virulence genes. Furthermore, selected S. Typhimurium isolates (n = 4) carrying different resistance and virulence genes up-regulated the secretions of cytokines IL-6 and IL-8 by Caco-2 cells in different degrees, suggesting that virulence genes may play a role in inflammatory transcription. In in vivo virulence test, microbiological counts in mouse feces and tissues showed that all included S. Typhimurium were able to infect mice, with one strain showing significantly higher virulence than others. In conclusion, this study indicates Salmonella contamination in fresh foods in Hangzhou market poses a risk to public health and it should be closely monitored to prevent and control foodborne diseases.

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Section: Methodsmentioning

confidence: 99%

Isolation, antimicrobial resistance and virulence characterization of Salmonella spp. from fresh foods in retail markets in Hangzhou, China

Qian,

Xu,

Wang

et al. 2023

PLoS ONE

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“…In addition, there is a strong industrial demand to comply with legislation and Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon the rapid release of food products to the market. Recent advances in technologies for the detection and identification of contaminated foodborne pathogens have provided faster, more sensitive and specific alternatives to conventional methods (Paula, et al 2002;Kawasaki, et al, 2009;Koo, et al, 2016;Suo, et al, 2017) These assays are generally referred as "fast" or alternative methods, terms commonly used to describe a variety of assays including miniaturized biochemical kits, immunoassays, DNA/ RNA-based assays, and combinations with cultural methods (Lofstrom et al, 2010;Almeida et al, 2013;Kim, et al, 2017). Molecular testing uses a specific sequence of bacterial nucleic acids as a target for pathogen detection and this is the category of alternative methods that most improved in recent years (Valderrama et al, 2016;Hu, et al, 2018;Bundidamorn, et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon

et al. 2021

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Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assurance and tracking infectious agents within the chain. In this context, this study aimed to evaluate the equivalence of two rapid detection tests, as alternative methods to the conventional Salmonella detection method, as well as to verify the microbiological quality parameters of two commercially important fish species in the Amazon biome. The plate count of aerobic bacteria ranged from 7.76 x 10 4 to 8.71 x 10 7 CFU.g-1 for mesophiles and 1.70 x 10 6 to 4.27 x 10 8 CFU.g-1 for psychrotrophic whereas the maximum for this group of microorganisms in fresh fish is 10 6 CFU.g-1. Regarding the Staphylococcus count, the two species presented variations between 1.35 x 10 4 to 1.51 x 10 5 CFU.g-1. This represents unsatisfactory conditions of handling, storage and conservation of fish species. The immunoenzymatic and molecular methods have been shown to be reliable, fast and effective in the detection of Salmonella and for its high index of agreement with the conventional detection method. We also emphasize the convenience of multiplex PCR application due to the high sensitivity, specificity, speed and accuracy of Salmonella detection.

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A new single-tube platform of melting temperature curve analysis based on multiplex real-time PCR using EvaGreen for simultaneous screening detection of Shiga toxin-producing Escherichia coli, Salmonella spp. and Listeria monocytogenes in food

2018

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Comparison of DNA isolation methods for detection of foodborne pathogens by real-time PCR from foods

Cited by 5 publications

References 17 publications

Isolation, antimicrobial resistance and virulence characterization of Salmonella spp. from fresh foods in retail markets in Hangzhou, China

Isolation, antimicrobial resistance and virulence characterization of Salmonella spp. from fresh foods in retail markets in Hangzhou, China

Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon

A new single-tube platform of melting temperature curve analysis based on multiplex real-time PCR using EvaGreen for simultaneous screening detection of Shiga toxin-producing Escherichia coli, Salmonella spp. and Listeria monocytogenes in food

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