Comparison of dot enzyme-linked immunosorbent assay (dot-ELISA), passive haemagglutination test (PHT) and thin layer immunoassay (TIA) in the diagnosis of natural or experimental fasciola hepatica infections in sheep

Morilla, Camila Arriaga De; Paniagua, Ricardo; Ruíz-Navarrete, Angela; Bautista, Carlos R.; Morilla, Antonio

doi:10.1016/0304-4017(89)90015-0

Cited by 16 publications

(7 citation statements)

References 11 publications

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“…While we cannot know whether those were animals with previous exposure to F. hepatica this may be an indication of cross-reactivity which can be further supported by Ibarra et al (1998) who reported cross-reactivity of an ES antigen ELISA first described by Arriaga de Morilla et al in 1989 with Paramphistomum spp. [45]. …”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Performance of Five Diagnostic Tests for Fasciola hepatica Infection in Naturally Infected Cattle Using a Bayesian No Gold Standard Approach

et al. 2016

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The clinical and economic importance of fasciolosis has been recognised for centuries, yet diagnostic tests available for cattle are far from perfect. Test evaluation has mainly been carried out using gold standard approaches or under experimental settings, the limitations of which are well known. In this study, a Bayesian no gold standard approach was used to estimate the diagnostic sensitivity and specificity of five tests for fasciolosis in cattle. These included detailed liver necropsy including gall bladder egg count, faecal egg counting, a commercially available copro-antigen ELISA, an in-house serum excretory/secretory antibody ELISA and routine abattoir liver inspection. In total 619 cattle slaughtered at one of Scotland’s biggest abattoirs were sampled, during three sampling periods spanning summer 2013, winter 2014 and autumn 2014. Test sensitivities and specificities were estimated using an extension of the Hui Walter no gold standard model, where estimates were allowed to vary between seasons if tests were a priori believed to perform differently for any reason. The results of this analysis provide novel information on the performance of these tests in a naturally infected cattle population and at different times of the year where different levels of acute or chronic infection are expected. Accurate estimates of sensitivity and specificity will allow for routine abattoir liver inspection to be used as a tool for monitoring the epidemiology of F. hepatica as well as evaluating herd health planning. Furthermore, the results provide evidence to suggest that the copro-antigen ELISA does not cross-react with Calicophoron daubneyi rumen fluke parasites, while the serum antibody ELISA does.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Performance of Five Diagnostic Tests for Fasciola hepatica Infection in Naturally Infected Cattle Using a Bayesian No Gold Standard Approach

et al. 2016

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“…Several groups reported the use of dot-ELISA for screening anti-Fasciola antibodies in cattle (Ibarra et al 1998;Maisonnave 1999;Castro et al 2000), in llamas (Rickard 1995), in sheep (Zimmerman et al 1985;Arriaga de Morilla et al 1989), and in rabbits (Rivera Marrero et al 1988). This serodiagnostic test has also been applied to diagnose human fasciolosis with an emphasis on crude helminthic antigens (Itoh and Sato 1990;Shaheen et al 1994) or partially purified antigen of somatic adult worms (Shaheen et al 1989).…”

Section: Discussionmentioning

confidence: 98%

Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

Ali

2011

Parasitol Res

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For the diagnosis of human fasciolosis, a new dipstick assay was developed and its diagnostic efficacy was evaluated in comparison to enzyme linked immunosorbent assay (ELISA) using Fasciola gigantica excretory-secretory antigen. Sera samples were collected from patients with confirmed fasciolosis and patients with other parasitic diseases as well as from normal healthy individuals. The sensitivity, specificity, and diagnostic accuracy of the dipstick assay as well as ELISA were 100%, 96.7%, and 98.3%, respectively. The dipstick assay is extremely easy to apply, rapid (15 min), with a visually interpreted result, field portable, inexpensive, reagent conservative, and as being both sensitive and specific in diagnosis of human fasciolosis; the test could be an acceptable alternative for use in clinical laboratories lacking specialized equipment needed for ELISA.

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“…This technique provides a useful method for the diagnosis of fasciolosis or the evaluation of an anthelmintic treatment because many sera can be processed and reactants are reasonably priced (Arriaga de Morilla et al 1989). The main inconvenience of the indirect ELISA is the long persistence of elevated titres of antibodies, even when flukes have been removed (Espino et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and expression of a Fasciola hepatica cDNA encoding a 2.9-kDa recombinant protein for the diagnosis of ovine fasciolosis

et al. 2004

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A 400-bp Fasciola hepatica cDNA clone was isolated from an expression library by immunological screening using rat sera taken 2 weeks after experimental infection. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 78 bp which encoded a 25 amino acid polypeptide with a predicted molecular weight of 2.9 kDa. This polypeptide was expressed in bacteria as a GST-fusion protein and used for the production of specific antigen. The 2.9 kDa recombinant protein (APS) was evaluated against sera from experimentally infected sheep using an indirect ELISA, and the results were compared to those obtained using F. hepatica excretory/secretory products (ESP). The pattern of IgG was very similar both against the recombinant and the native proteins, increasing early following the infection. After treatment with triclabendazole, the IgG response against the APS seroreverted to negative values, whereas it remained elevated against the ESP. We conclude that this recombinant protein could be used in diagnostic assays for the identification of recently infected sheep.

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Comparison of dot enzyme-linked immunosorbent assay (dot-ELISA), passive haemagglutination test (PHT) and thin layer immunoassay (TIA) in the diagnosis of natural or experimental fasciola hepatica infections in sheep

Cited by 16 publications

References 11 publications

Evaluation of the Performance of Five Diagnostic Tests for Fasciola hepatica Infection in Naturally Infected Cattle Using a Bayesian No Gold Standard Approach

Evaluation of the Performance of Five Diagnostic Tests for Fasciola hepatica Infection in Naturally Infected Cattle Using a Bayesian No Gold Standard Approach

Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

Isolation, identification and expression of a Fasciola hepatica cDNA encoding a 2.9-kDa recombinant protein for the diagnosis of ovine fasciolosis

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