Comparison of drug release from liquid crystalline monoolein dispersions and solid lipid nanoparticles using a flow cytometric technique

Abstract

Colloidal lipid particles such as solid lipid nanoparticles and liquid crystalline nanoparticles have great opportunities as drug carriers especially for lipophilic drugs intended for intravenous administration. In order to evaluate drug release from these nanoparticles and determine their behavior after administration, emulsion droplets were used as a lipophilic compartment to which the transfer of a model drug was measured. The detection of the model drug transferred from monoolein cubic particles and trimyristin solid lipid nanoparticles into emulsion droplets was performed using a flow cytometric technique. A higher rate and amount of porphyrin transfer from the solid lipid nanoparticles compared to the monoolein cubic particles was observed. This difference might be attributed to the formation of a highly ordered particle which leads to the expulsion of drug to the surface of the crystalline particle. Furthermore, the sponge-like structure of the monoolein cubic particles decreases the rate and amount of drug transferred. In conclusion, the flow cytometric technique is a suitable technique to study drug transfer from these carriers to large lipophilic acceptors. Monoolein cubic particles with their unique structure can be used successfully as a drug carrier with slow drug release compared with trimyristin nanoparticles.

Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity

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O/W emulsions stabilised by solid lipid particles: Understanding how the particles’ Pickering functionality can be retained post their dehydration and subsequent rehydration

“…The observed reduction in the interfacial tension of the reconstituted systems suggests that a proportion of the protein content, assumed to be associated with the wax particles prior to freeze-drying, has now been expelled. Several studies in literature concerning drug-loaded solid lipid nanoparticles have reported on the expulsion of the drug molecule to the surface of the crystalline particle as a result of a highly ordered structure (Westesen, Bunjes, & Koch, 1997;zur Mühlen, Schwarz, & Mehnert, 1998;Dawoud & Nasr, 2016). This tendency could also be the case in the investigated lipid particulate systems, which crystallise in the most stable polymorphic forms allowing little space, particularly for a large protein molecule to fit in.…”

Preclinical assessment of nanostructured liquid crystalline particles for the management of bacterial keratitis: in vivo and pharmacokinetics study

“…Pure poloxamer 407 exhibited characteristic broad peak at 3425 cm − 1 due to O-H group stretching, 2889 cm − 1 peak indicated C-H stretching vibration, sharp peak at 1596 cm − 1 corresponded to C-C stretching and sharp peak at 1384 cm − 1 indicated nitrate absorption band. Peak at 1113 cm − 1 corresponded to C-O stretching vibration and slight peak at 766 cm − 1 was related to C-N stretch of aromatic and peak at 618 cm − 1 were due to C-H bending [41,42].…”