Comparison of earthworm responses to petroleum hydrocarbon exposure in aged field contaminated soil using traditional ecotoxicity endpoints and 1H NMR-based metabolomics

Åslund, Melissa Whitfield; Stephenson, Gerald R.; Simpson, André J.; Simpson, Myrna J.

doi:10.1016/j.envpol.2013.07.026

Cited by 23 publications

(6 citation statements)

References 42 publications

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“…This method is fast and can determine quantitative changes in the levels of metabolites, such as amino acids and sugars, provoked by an external stressor [26,27,28,29,30]. Being an untargeted technique, such an analysis can be reproduced accurately across different laboratories [31,32,33,34,35,36], providing insights into the reaction of the organism to an external source of stress, its molecular endpoints in terms of changes in metabolite levels, and in depicting the mode of toxicity of the contaminants [37].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Zhang

Zhou

2019

Molecules

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Although metalaxyl and metalaxyl-M are widely used fungicides, very little is known about their subacute and enantiospecific effects on the earthworm metabolome. In this study, Eisenia fetida were exposed to metalaxyl and metalaxyl-M at three concentrations (0.5, 5 and 50 mg/kg) for seven days. 1H nuclear magnetic resonance (1H-NMR)-based untargeted metabolomics showed that metalaxyl and metalaxyl-M exposure disturbed earthworms’ metabolism at all three concentrations. Endogenous metabolites, such as succinate, arginine, aspartate, urea, asparagine, alanine, trimethylamine, taurine, cysteine, serine, threonine, histidine, lysine, glucose, choline, carnitine, citric acid, alpha-ketoisovaleric acid, fumaric acid and so on, were significantly changed. These results indicate that metalaxyl and metalaxyl-M produce different, enantiospecific disturbances in the earthworm metabolism, particularly in the tricarboxylic acid (TCA) and urea cycles. The application of untargeted metabolomics thus provides more information for evaluating the toxic risks of metalaxyl and metalaxyl-M.

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Section: Introductionmentioning

confidence: 99%

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Zhang

Zhou

2019

Molecules

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“…These types of studies are very informative and provide toxicological endpoints but do not always provide information about the toxic mode of action of the pollutant at sublethal levels [1]. A promising tool that has emerged to assess organism responses to sublethal pollutant exposure is 1 H nuclear magnetic resonance (NMR) metabolomics [1,[5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. This technique quickly and quantitatively measures changes in a range of metabolites such as sugars and amino acids in response to an external stressor [20].…”

Section: Introductionmentioning

confidence: 99%

Analysis of earthworm sublethal toxic responses to atrazine exposure using ¹H nuclear magnetic resonance (NMR)‐based metabolomics

Dani

Simpson

2017

Enviro Toxic and Chemistry

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Atrazine toxicity to earthworms is still not fully understood, particularly at sublethal concentrations. Because of the ubiquity of atrazine in the environment, it is imperative to understand the impacts of atrazine presence to soil-dwelling organisms. To examine this in detail, we used H nuclear magnetic resonance (NMR)-based metabolomics to elucidate earthworm (Eisenia fetida) responses after 48 h of atrazine exposure in contact tests. Earthworms were exposed to 4 sublethal concentrations of 362.4, 181.2, 90.6, and 45.3 ng/cm , which correspond to 1/8th, 1/16th, 1/32nd, and 1/64th of the median lethal concentration (LC50) values, respectively. After exposure, polar metabolites were isolated from earthworm tissues and analyzed using H NMR spectroscopy. Sublethal atrazine exposure induced a nonmonotonic response with respect to exposure concentration and caused an overall suppression in earthworm metabolism. Maltose, fumarate, malate, threonine/lactate, adenosine-5'-triphosphate (ATP), betaine, scyllo-inositol, glutamate, arginine, and glutamine were the metabolites identified as most sensitive to atrazine exposure. These observed fluctuations in the metabolic profile suggest that atrazine reduced ATP synthesis and negatively impacted the health of earthworms after acute sublethal exposure. Our study also demonstrates the utility of NMR-based metabolomics for the basic assessment of sublethal toxicity, which can then be used for more targeted approaches with other molecular techniques. Environ Toxicol Chem 2018;37:473-480. © 2017 SETAC.

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“…On the contrary, other authors reported that petroleum concentration of 9.5 g/kg resulted in high mortality of E. fetida, А. chlorotica and L. terrestris in soils without organic matter after 28 days of colonization [12]. Whitfield Aslund et al, 2013, observed the absence of petroleum-contaminated soil acute toxicity for worms E. fetida and 90% survival at low petroleum concentrations (to 20 g/kg), but failure of the reproduction as well [13]. Eom I.C.…”

Section: Introductionmentioning

confidence: 93%

Biological Remediation of the Engine Lubricant Oil-contaminated Soil with Three Kinds of Earthworms, Eisenia Fetida, Eisenia Andrei, Dendrobena Veneta, and a Mixture of Microorganisms

Чачина

Воронкова

Бакланова

2015

Procedia Engineering

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The survival of the earthworms in used engine oil-contaminated soil with concentration of 20-100 g/kg was estimated. The investigation was made of the contaminated soil bioremediation efficiency with the use of Eisenia fetida, Eisenia andrei and Dendrobena veneta in presence of the photosynthetic bacteria Thiorhodaceae, Athiorhodaceae and Chlorobacteriaceae, nitrogenfixing bacteria Azotobacter and Clostridium, yeast cells Saccharomyces, fungi Aspergillus and Penicillium, and Actinomycetales, composing microbiologic product Baykal EM. It is determined that the used oil concentration in the soils with earthworms decreases 4-10 times faster than in contaminated soil without earthworms. High rates of engine oil removal are registered in presence of E. andrei and D. veneta. Oil concentration in soil decreased by 60-90%. With oil concentration of 60-100 g/kg and earthworms (Eisenia fetida) cultivation, the oil concentration decreased 4-6 times, with introduction of Californian earthworms (Eisenia andrei) 12 times and with Dendrobena veneta 12-23 times. At the addition of the microbiologic product along with earthworms, oil content in the soil decreased 58 times and constituted 1.1 g/kg, soil remediation efficiency being 99.9%.

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Comparison of earthworm responses to petroleum hydrocarbon exposure in aged field contaminated soil using traditional ecotoxicity endpoints and 1H NMR-based metabolomics

Cited by 23 publications

References 42 publications

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Analysis of earthworm sublethal toxic responses to atrazine exposure using ¹H nuclear magnetic resonance (NMR)‐based metabolomics

Biological Remediation of the Engine Lubricant Oil-contaminated Soil with Three Kinds of Earthworms, Eisenia Fetida, Eisenia Andrei, Dendrobena Veneta, and a Mixture of Microorganisms

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Comparison of earthworm responses to petroleum hydrocarbon exposure in aged field contaminated soil using traditional ecotoxicity endpoints and 1H NMR-based metabolomics

Cited by 23 publications

References 42 publications

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Analysis of earthworm sublethal toxic responses to atrazine exposure using 1H nuclear magnetic resonance (NMR)‐based metabolomics

Biological Remediation of the Engine Lubricant Oil-contaminated Soil with Three Kinds of Earthworms, Eisenia Fetida, Eisenia Andrei, Dendrobena Veneta, and a Mixture of Microorganisms

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Analysis of earthworm sublethal toxic responses to atrazine exposure using ¹H nuclear magnetic resonance (NMR)‐based metabolomics