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The survival of the earthworms in used engine oil-contaminated soil with concentration of 20-100 g/kg was estimated. The investigation was made of the contaminated soil bioremediation efficiency with the use of Eisenia fetida, Eisenia andrei and Dendrobena veneta in presence of the photosynthetic bacteria Thiorhodaceae, Athiorhodaceae and Chlorobacteriaceae, nitrogenfixing bacteria Azotobacter and Clostridium, yeast cells Saccharomyces, fungi Aspergillus and Penicillium, and Actinomycetales, composing microbiologic product Baykal EM. It is determined that the used oil concentration in the soils with earthworms decreases 4-10 times faster than in contaminated soil without earthworms. High rates of engine oil removal are registered in presence of E. andrei and D. veneta. Oil concentration in soil decreased by 60-90%. With oil concentration of 60-100 g/kg and earthworms (Eisenia fetida) cultivation, the oil concentration decreased 4-6 times, with introduction of Californian earthworms (Eisenia andrei) 12 times and with Dendrobena veneta 12-23 times. At the addition of the microbiologic product along with earthworms, oil content in the soil decreased 58 times and constituted 1.1 g/kg, soil remediation efficiency being 99.9%.

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The recultivation technology for soils contaminated with oil in the concentration of 50 g/kg in Western Siberia with the use of microbiological preparation received from coprolites of earthworms Eisenia fetida and vermiculture of earthworms E. fetida, E. andrei, D. veneta was developed. The coprolites microflora composition of the earthworms E. fetida was studied. The microbiological preparation from coprolites of the earthworms was received. Microorganisms – oil destructors were isolated, which were used as the basis of the microbiological preparation “Muck” containing Pseudomonas aeroginosa, Bacillus careus, Bacillus mucoides, Bacillus thuringiens, Pseudomonas putida, CFU – 3.3*108. The following characteristics were determined: the remediation effectiveness for the soil contaminated with oil in the concentration of 50 g/kg when using the microbiological preparation “Muck” and earthworms E. fetida, E. Andrei and D. veneta.