The recultivation technology for soils contaminated with oil in the concentration of 50 g/kg in Western Siberia with the use of microbiological preparation received from coprolites of earthworms Eisenia fetida and vermiculture of earthworms E. fetida, E. andrei, D. veneta was developed. The coprolites microflora composition of the earthworms E. fetida was studied. The microbiological preparation from coprolites of the earthworms was received. Microorganisms – oil destructors were isolated, which were used as the basis of the microbiological preparation “Muck” containing Pseudomonas aeroginosa, Bacillus careus, Bacillus mucoides, Bacillus thuringiens, Pseudomonas putida, CFU – 3.3*108. The following characteristics were determined: the remediation effectiveness for the soil contaminated with oil in the concentration of 50 g/kg when using the microbiological preparation “Muck” and earthworms E. fetida, E. Andrei and D. veneta.

Food processing (FP) industries are dominated by thermal inputs for food preservation. Such treatments can, however, contribute to some undesirable changes in the quality attributes and nutritional value of food. Non-thermal storage processes, on the other hand, have a negligible effect on the sensory consistency and nutritional status of food. Food preservation has used food with non-thermal methods with the aim of preserving the nutritious properties and physic-chemical features of foods. In this respect, the pulsed electrical field offers an alternate option for different food items, in particular liquid food products, in order to improve the protection and maintenance of new, consistent aspects of food. Similarly, this study emphasizes the promise of pulsed electric field technology as a useful method for preserving a variety of foods, with a focus on microbial inactivation.The key goal of this research is to find out how widely nonthermal FP equipment is used. Innovative technology can be able to help strike the right balance between protection and minimum processing, as well as sufficient economic constraints and superior efficiency.

The article investigates bioremediation efficiency of the soil contaminated with petroleum (20 to 60 g/kg) and diesel fuel (20 to 40 g/kg) with the help of earthworms Dendrobena veneta in the presence of bacteria Paenibacillus pabuli, Azotobacter vinelandii, Lactobacillus casei, Clostridium limosum, Cronobacter sakazakii, Rhodotorulla mucilaginosa, Cryptococcus albidus, eria, yeasts Saccharomyces, fungi Aspergillus and Penicillium, as well as Actinomycetales, all being components of biopreparation Baykal-EM. It was demonstrated that in oil-contaminated soil, the content of hydrocarbons decreased by 95 % after 22 weeks in the presence of worms and bacteria. The microbiological preparation introduction increased the earthworms’ survivability in oil-contaminated substrate. Microbiological preparation introduction improved the earthworms’ survival in contaminated substrate. The microbiological preparation introduction, therefore, allowed to use Dendrobena veneta for soil recultivation with higher oil concentrations that was impossible in the past. In petroleum-contaminated soil the content of hydrocarbons decreased by 97% after 5 months. The presence of the diesel fuel in the amount of 40 g per 1 kg soil had an acute toxic effect and caused the death of 90 % earthworm species in 14 days. Bacteria introduction enhanced the toxic effect of the diesel fuel and resulted in the death of 100 % earthworms after 7 days.

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