Comparison of efficacy and toxicity of bevacizumab, endostar and apatinib in transgenic and human lung cancer xenograftzebrafish model

Wei, Lingxiao; Jiang, Qi; Song, Xiaowei; Teng, Chong; Fan, Chengjuan; Lv, Yanju; Liu, Ying; Shen, Weitao; Li, Li; Huang, Dayong; Xin, Tao

doi:10.1038/s41598-018-34030-5

Cited by 29 publications

(20 citation statements)

References 50 publications

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“…Besides, the tested four DKPs had a promising antitumor effect on HT-29 xenograft zebrafish. In agreement with our findings, three anti-angiogenic drugs such as bevacizumab, endostar, and lapatinib were demonstrating a remarkable inhibition of tumor growth in a xenografted zebrafish model within a nonlethal dose range 44 . Fu and co-workers reported that pan-phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 significantly reduced the xenograft tumor size in zebrafish by decreasing the viability of metastatic cancer cells 45 .…”

Section: Discussionsupporting

confidence: 92%

Induction of mitochondria-mediated apoptosis and suppression of tumor growth in zebrafish xenograft model by cyclic dipeptides identified from Exiguobacterium acetylicum

Sekar

Teng

Dahms‬

et al. 2020

Sci Rep

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ponnusamy 5 , charles chien-chih chiu 2 , B. S. Dileep Kumar 6* & natesan Sivakumar 1* colorectal cancer is the most common type of gastrointestinal cancers with poor survival and limited therapeutic options. In this study, four structurally different cyclic dipeptides (or diketopiperazine) were isolated and identified as cyclo (l-pro-l-Leu), cyclo (l-pro-l-Val), cyclo (l-pro-l-Phe) and cyclo (l-pro-l-Tyr) from the ethyl acetate extract in the cell-free filtrate of Exiguobacterium acetylicum S01. The anticancer potential of identified DKPs on colorectal cancer HT-29 cells in vitro and in vivo zebrafish xenograft model was evaluated. The MTT (3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide)) assay showed that four DKPs exhibited significant inhibition of HT-29 cells viability in a dose-dependent manner whereas there were no cytotoxic effects on normal mouse fibroblast 3T3 cells. Also, we observed that all DKPs induce early and late apoptotic cell death in HT-29 cells. Moreover, the expression levels of apoptotic (cytochrome-c, caspase-3 and Bid) and anti-apoptotic (Bcl-2) markers were up-and down-regulated in HT-29 cells in response to DKPs treatments. Furthermore, these four DKPs remarkably inhibited the tumor progression in a zebrafish xenograft model within a nonlethal dose range. Overall, our findings suggest that cyclic dipeptides derived from E. acetylicum S01 could be promising chemopreventive/ therapeutic candidates against cancer. Colorectal cancer (CRC) is the third most commonly diagnosed malignancy and the second leading cause of cancer-related deaths in the world. According to the World Health Organization reports, the global burden of CRC is expected to increase by 60% i.e., 2.2 million new cases and 1.1 million deaths by the year 2030 1. The risk factor for developing CRC is connected with age, genetic predisposition, disease history, and lifestyle including

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Section: Discussionsupporting

confidence: 92%

Induction of mitochondria-mediated apoptosis and suppression of tumor growth in zebrafish xenograft model by cyclic dipeptides identified from Exiguobacterium acetylicum

Sekar

Teng

Dahms‬

et al. 2020

Sci Rep

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“…In animal models, the antitumor activity of Endostar is more preponderant than bevacizumab, while they show comparative antiangiogenic effects . The traditional method of Endostar administration reduces patient compliance and affects quality of life.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Endostar continuous versus intermittent intravenous infusion in combination with first‐line chemotherapy in patients with advanced non‐small cell lung cancer

et al. 2019

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Background Intravenous infusion of Endostar for three to four hours per day for 14 days reduces patient compliance and affects quality of life. Continuous intravenous infusion (CI) represents a novel method of administration; however, it is unclear whether it is effective and safe when compared to the traditional method. Methods We retrospectively reviewed patients with advanced non‐small cell lung cancer (NSCLC) administered CI (20 patients) or intermittent intravenous infusion (II, 49 patients) of Endostar combined with first‐line chemotherapy. Three patients in the II group discontinued therapy because of adverse effects. Results Median progression‐free survival was 6.0 months in the CI group and 3.8 months in the II group, with no significant difference (P = 0.1). The objective response and disease control rates were also similar in the CI and II groups (40.0 vs. 32.6%, P = 0.562; 65 vs. 69.6%, P = 0.714, respectively). Conclusion CI of Endostar combined with first‐line chemotherapy for advanced NSCLC had similar progression‐free survival, objective response, and overall response rates as II, with tolerable adverse effects.

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“…A zebrafish subintestinal vessel angiogenesis assay was performed according to a previously reported method . Zebrafish embryos were injected with different concentrations of Tp47 (22, 44 or 88 ng/fish) or PBS (control group).…”

Section: Methodsmentioning

confidence: 99%

Recombinant Treponema pallidum protein Tp47 induces angiogenesis by modulating the matrix metalloproteinase/tissue inhibitor of metalloproteinase balance in endothelial cells

Gao

Luo

Liu

et al. 2019

Acad Dermatol Venereol

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Background Although angiogenesis is an obvious pathological manifestation in the pathogenesis of syphilis, little is known about the underlying mechanisms of angiogenesis induced by reactions to Treponema pallidum antigens.Objective In this study, we sought to determine the role of recombinant T. pallidum Tp47 in promoting angiogenesis in endothelial cells and the related mechanism.Methods Evaluation of the pro-angiogenic activity of recombinant T. pallidum Tp47 in human umbilical vein endothelial cells (HUVECs) was assessed, and the balance of matrix metalloproteinase (MMP)/tissue inhibitor of metalloproteinase (TIMP) and the mechanisms underlying the involvement of Akt/mTOR/S6 pathways in this process were explored.Results Under stimulation by Tp47, HUVECs exhibited obvious proliferation, migration and tube formation. In addition, the apparent promotion of angiogenesis by Tp47 was observed using a zebrafish embryo model. During angiogenesis, the levels of MMP-1 and MMP-10 were significantly elevated, whereas those of TIMP-1 and TIMP-2 did not change. In addition, after transfection with siRNAMMP-1 and siRNAMMP-10, migration and tube formation were significantly inhibited. Akt/mTOR/S6 signalling was found to be involved in upregulating MMP-1 and MMP-10 expression, and the sequential blockade of steps in the pathways effectively prevented Tp47-induced angiogenesis. ConclusionThe results reveal the underlying mechanism of angiogenesis promoted by Tp47, namely, upregulating MMP-1 and MMP-10 expression to disrupt the MMP/TIMP balance through the Akt/mTOR/S6 pathway. These findings contribute to our understanding of the pathophysiology of syphilis. At the second stage (secondary syphilis), T. pallidum causes a plethora of clinical manifestations characterized by vascular inflammation and increased angiogenesis. 5,6 In tertiary syphilis, unresolved angiogenesis is the underlying mechanism responsible for skeletal changes. 7 Overall, angiogenesis appears to play a pivotal role in syphilis pathogenesis, as with other diseases (e.g. cancer, infections and immune disorders). 8 † These authors contributed equally to this work.

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Comparison of efficacy and toxicity of bevacizumab, endostar and apatinib in transgenic and human lung cancer xenograftzebrafish model

Cited by 29 publications

References 50 publications

Induction of mitochondria-mediated apoptosis and suppression of tumor growth in zebrafish xenograft model by cyclic dipeptides identified from Exiguobacterium acetylicum

Induction of mitochondria-mediated apoptosis and suppression of tumor growth in zebrafish xenograft model by cyclic dipeptides identified from Exiguobacterium acetylicum

Comparison of Endostar continuous versus intermittent intravenous infusion in combination with first‐line chemotherapy in patients with advanced non‐small cell lung cancer

Recombinant Treponema pallidum protein Tp47 induces angiogenesis by modulating the matrix metalloproteinase/tissue inhibitor of metalloproteinase balance in endothelial cells

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