Comparison of ELISA and TLC Methods for the Morphine Detection in Urine of Drug Abusers

Alireza, Timcheh Hariri; Mahdi, Balali Mood; Sadeghi, Mahmood; Lari, Niloofar; Bamdad, Riahi Zanjani

doi:10.29252/arakmu.10.3.47

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…The previously mentioned drugs could result in drug interaction mainly in the rapid strip tests and to less extent in EMIT leading to false positive or false negative results when compared with the TLC technique. This has coincided with Timcheh-Hariri et al (13) who reported higher specificity of the TLC technique in detecting false-positive results by immunoassays.…”

Section: Discussionsupporting

confidence: 78%

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Screening of Opioid Abuse in Toxicology Unit and Laboratory at Mansoura University with A Comparison of Two Detection Methods: Clinical and Legal Issues

Badawy¹,

El-Kattan²,

El-Mosallamy³

2022

The Egyptian Journal of Hospital Medicine

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Background: Drug abuse is a critical concern worldwide. Urine drug screening is a common practice applied for the detection of common drugs of abuse for both clinical and legal issues. Objective: This study was performed to screen for opioid abuse in urine samples collected from adult subjects (aged > 18 years) at the toxicology laboratory and toxicology unit, Emergency Hospital, Mansoura University, and to compare enzyme-multiplied immunoassay (EMIT) with thin layer chromatography (TLC) techniques for confirmation of opiate in samples. Patients and Methods: A total of 500 urine samples were randomly collected and screened with immunochromatographic (rapid) test strips for opiates. The positive urine samples obtained by the rapid test were further analyzed by EMIT and TLC techniques. Results: A total of 80 samples (16%) were positive for opiate by the rapid test. By analyzing these positive samples using EMIT, only 68 samples were positive (68/80; 85%). Only 66 samples (66/80; 82.5%) revealed positive results (opiate spot) meaning that TLC was able to confirm 2 false-positive results by EMIT. This might be due to drug interactions. Conclusion: Opioid abuse seems to be a problem among the Egyptian adult population. Despite the high sensitivity of EMIT (100%), TLC is more specific when compared with it. Indeed, EMIT can be a relatively accurate alternative for TLC, but, whenever positive results need to be re-checked, it is recommended to use the cheap and widely available TLC technique or ideally use the High-performance liquid chromatography (HPLC), particularly for legal purposes.

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Section: Discussionsupporting

confidence: 78%

“…According to manufacturers' claims, no crossreactivity was found for many non-related medications to morphine derivatives at different concentrations in urine. Still, related compounds to morphine had been analyzed by the qualitative method and cross-reactivity could exist (13) .…”

Section: Discussionmentioning

confidence: 99%

Screening of Opioid Abuse in Toxicology Unit and Laboratory at Mansoura University with A Comparison of Two Detection Methods: Clinical and Legal Issues

Badawy¹,

El-Kattan²,

El-Mosallamy³

2022

The Egyptian Journal of Hospital Medicine

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“…In these studies, it was also concluded that although TLC is more specific, it is more time-consuming and less-sensitive, in comparison to ELISA. It is worth mentioning that although TLC is an old method, it is much more reliable than ELISA (10).…”

Section: Discussionmentioning

confidence: 99%

The Study of Ranitidine Interference With Morphine Detection Test by Thin-Layer Chromatography

Miri

Nadi

Zeraati

2018

Avicenna J Med Biochem

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Background: Drug abuse is a global and critical problem. One of the most frequent practices done in order to detect the drugs of abuse is Urine Drug Screen. However, for changing the drug test results, adulterants and urine substitutes are being designed. As the referring people’s background has shown, ranitidine is one of the interfering drugs in morphine detection test. Therefore, in the present study, the interference of ranitidine in morphine detection test will be studied. Methods: Ten healthy volunteers who had not used any kind of drug for 72 hours before the test were recruited into the study. First, 2 doses of ranitidine (150 and 300 mg) were administered to the subjects orally and 100-mL urine samples were collected from them before and after taking ranitidine. The second urine sample was collected at 6-8 am. Ten micrograms morphine was added to both urine samples of each individual. The urine samples were tested using thin-layer chromatography (TLC) technique. The experiment was repeated after 1 week using ranitidine 300 mg. Results: The TLC test was carried out on 40 urine samples. Twenty samples were tested before and, the rest, after ranitidine consumption. The TLC test results were positive before ranitidine consumption but negative for 18 samples and positive for two samples after taking ranitidine. Conclusion: Ranitidine may change the urine morphine screening test results via TLC method and induce a false negative result.

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Noble metal nanostructures in optical biosensors: Basics, and their introduction to anti-doping detection

Malekzad¹,

Zangabad

Mohammadi

et al. 2018

TrAC Trends in Analytical Chemistry

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Nanotechnology has illustrated significant potentials in biomolecular-sensing applications; particularly its introduction to anti-doping detection is of great importance. Illicit recreational drugs, substances that can be potentially abused, and drugs with dosage limitations according to the prohibited lists announced by the World Antidoping Agency (WADA) are becoming of increasing interest to forensic chemists. In this review, the theoretical principles of optical biosensors based on noble metal nanoparticles, and the transduction mechanism of commonly-applied plasmonic biosensors are covered. We review different classes of recently-developed plasmonic biosensors for analytic determination and quantification of illicit drugs in anti-doping applications. The important classes of illicit drugs include anabolic steroids, opioids, stimulants, and peptide hormones. The main emphasis is on the advantages that noble metal nano-particles bring to optical biosensors for signal enhancement and the development of highly sensitive (label-free) biosensors. In the near future, such optical biosensors may be an invaluable substitute for conventional anti-doping detection methods such as chromatography-based approaches, and may even be commercialized for routine anti-doping tests.

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Comparison of ELISA and TLC Methods for the Morphine Detection in Urine of Drug Abusers

Abstract: Background:The current study was conducted to compare ELISA with thin layer

Cited by 5 publications

References 8 publications

Screening of Opioid Abuse in Toxicology Unit and Laboratory at Mansoura University with A Comparison of Two Detection Methods: Clinical and Legal Issues

Screening of Opioid Abuse in Toxicology Unit and Laboratory at Mansoura University with A Comparison of Two Detection Methods: Clinical and Legal Issues

The Study of Ranitidine Interference With Morphine Detection Test by Thin-Layer Chromatography

Noble metal nanostructures in optical biosensors: Basics, and their introduction to anti-doping detection

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