Comparison of ELISA formats for detection of antibodies specific for nervous necrosis virus (Betanodavirus) in the serum of immunized barramundi Lates calcarifer and Australian bass Macquaria novemaculeata

Jaramillo, Diana; Hick, Paul; Deece, Kylie; Tweedie, Alison; Kirkland, Peter D.; Arzey, Edla; Whittington, Richard J.

doi:10.1016/j.aquaculture.2015.08.015

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…The major reasons for this are poor knowledge on the kinetics of the antibody response in fish at various water temperatures and lack of validation data. Nevertheless, several ELISA or serum neutralization tests described and improved over time proved their efficiency to detect antibodies specific to VNN (Watanabe et al 1998;Huang et al 2001;Fenner et al 2006b;Scapigliati et al 2010;Choi et al 2014;Jaramillo et al 2016b). For ELISA tests, the determination of the cut-off point is critical to make the distinction between virus free status and viral infection.…”

Section: Indirect Serological Methodsmentioning

confidence: 99%

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Viral encephalopathy and retinopathy in aquaculture: a review

Doan

Vandeputte

Chatain

et al. 2016

Journal of Fish Diseases

142

115

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Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a major devastating threat for aquatic animals. Betanodaviruses have been isolated in at least 70 aquatic animal species in marine and in freshwater environments throughout the world, with the notable exception of South America. In this review, the main features of betanodavirus, including its diversity, its distribution and its transmission modes in fish, are firstly presented. Then, the existing diagnosis and detection methods, as well as the different control procedures of this disease, are reviewed. Finally, the potential of selective breeding, including both conventional and genomic selection, as an opportunity to obtain resistant commercial populations, is examined.

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Section: Indirect Serological Methodsmentioning

confidence: 99%

“…; Jaramillo et al . ). For ELISA tests, the determination of the cut‐off point is critical to make the distinction between virus free status and viral infection.…”

Section: Diagnosis/detectionmentioning

confidence: 97%

Viral encephalopathy and retinopathy in aquaculture: a review

Doan

Vandeputte

Chatain

et al. 2016

Journal of Fish Diseases

142

115

View full text Add to dashboard Cite

show abstract

“…However, ELISA is problematic for detecting fish antibodies due to its low reproducibility and high background optical density (OD) [293,294], caused by non-specific reactions between antibodies and NNV particles [295]. To solve this problem, a sandwich ELISA using immobilized fish sera has recently been reported [295].…”

Section: Diagnostic Proceduresmentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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“…Denzin and Staak (2000) proposed that competitive ELISA with an alien species (mammalian or avian) was more effective than indirect ELISA at discriminating specific reactions. However, competitive ELISA may have lower sensitivity than indirect ELISA (Swain and Nayak 2003;Jaramillo et al 2015).…”

Section: Quality Controlmentioning

confidence: 99%

“…For every set of serum replicates tested with the antigen of interest, another set of replicates is tested without the antigen of interest. Thus, reactivity in the absence of the targeted antigen is an indicator of the relative specificity of the reaction-it indicates how much of the binding is directed towards the targeted antigen (Whittington et al 1994;Jaramillo et al 2015).…”

Section: Quality Controlmentioning

confidence: 99%

Serology in Finfish for Diagnosis, Surveillance, and Research: A Systematic Review

et al. 2017

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Historically, serological tests for finfish diseases have been underused when compared with their use in terrestrial animal health. For years the nonspecific immune response in fish was judged to make serology unreliable and inferior to the direct measurement of agent analytes. We conducted a systematic review of peer-reviewed publications that reported on the development, validation, or application of serological tests for finfish diseases. A total of 168 articles met the screening criteria; most of them were focused on salmonid pathogens (e.g., Aeromonas spp. and viral hemorrhagic septicemia virus). Before the 1980s, most publications reported the use of agglutination tests, but our review indicates that enzyme-linked immunosorbent assay (ELISA) has more recently become the dominant serological test. The main application of serological tests has been in the assessment of vaccine efficacy, with few applications for surveillance or demonstration of freedom from disease, despite the advantages of serological tests over direct detection at the population level. Nonlethal sampling, low cost, and postinfection persistence of antibodies make serological assays the test of choice in surveillance, especially of valuable broodstock. However, their adoption has been constrained by poor characterization and validation. The number of publications in our review reporting diagnostic sensitivity and specificity of serological tests in finfish was small (n = 7). Foreseeing a wider use of serological tests in the future for diagnostic end purposes, we offer recommendations for mitigating deficiencies in the development and evaluation of serological tests, including optimization, control of nonspecific reactions, informed cutoff points, diagnostic accuracy, and serological baseline studies. Achieving these goals will facilitate greater international recognition of serological testing in programs supporting aquatic animal health. Received March 21, 2016; accepted September 24, 2016.

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Comparison of ELISA formats for detection of antibodies specific for nervous necrosis virus (Betanodavirus) in the serum of immunized barramundi Lates calcarifer and Australian bass Macquaria novemaculeata

Cited by 16 publications

References 26 publications

Viral encephalopathy and retinopathy in aquaculture: a review

Viral encephalopathy and retinopathy in aquaculture: a review

Betanodavirus and VER Disease: A 30-year Research Review

Serology in Finfish for Diagnosis, Surveillance, and Research: A Systematic Review

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