Comparison of extraction solvents and sorbents in the quick, easy, cheap, effective, rugged, and safe method for the determination of pesticide multiresidue in fruits by ultra high performance liquid chromatography with tandem mass spectrometry

Abstract

An improved analytical method was developed for the simultaneous quantification of several plant growth regulators and fungicides (carbendazim, pyrimethanil, metalaxyl, triadimefon, paclobutrazol, thiophanate, prochloraz, dimethomorph, difenoconazole, (4-chlorophenoxy)-acetic acid, (2,4-dichlorophenoxy)-acetic acid, thiadiazuron, forchlorfenuron and gibberellins) in fruits followed by ultra high performance liquid chromatography with tandem mass spectrometry. Samples were extracted and purified using a modified QuEChERS method. Different extraction solvents and sorbents in the QuEChERS method were compared. Optimum results were followed by the addition of 1% acetic acid in acetonitrile; C18 sorbent was added due to the acidic nature of several pesticides. The recoveries of the pesticides were in the range 73.7-118.4%, with relative standard deviations lower than 16.63%. Limits of detection ranged from 0.1-1.0 μg/kg. The method presented here is simple, rapid, sensitive and can be applied to large-scale monitoring programs to screen the presences of pesticides in fruits.

Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach

“…Optimization of the sample purification method was a necessary step to remove the ME and obtain a good signal response. MEs (ion suppression and ion enhancement) were ubiquitous during the LC–MS/MS analysis due to the ionization competition between coeluting compounds in the system, causing loss of method accuracy, reproducibility, and detection, leading to incorrect analytical determinations . In this work, ME was evaluated using the strategy applied by Masuszewski et al.…”

Development and validation of an analytical method for multiresidue determination of pesticides in lettuce using QuEChERS–UHPLC–MS/MS

“…A simple, fast and, effective sample preparation approach for the determination of pesticide residues in fruits and vegetables QuEChERS was introduced by Anastassiades et al. in 2003 and has been widely accepted by the scientific community due to advantages such as low amounts of solvent and glassware required, wide analyte scope (including highly polar, acidic, and basic pesticides) and simple instrumentation, being successfully used for the determination of multiclass pesticides in many fruits and vegetables . The QuEChERS method consists in an initial extraction with an organic solvent followed by an extraction/partitioning step with the addition of a salt mixture.…”

Analysis of forchlorfenuron and thidiazuron in fruits and vegetables by surface‐enhanced Raman spectroscopy after selective solid‐phase extraction with modified β‐cyclodextrin

“…Therefore, the rapid determination of CPPU and TDZ in fruits and vegetables is of great significance. So far, chromatographic methods such as HPLC , GC–MS , and ELISA methods have been widely used for the detection of CPPU and TDZ in complex samples. However, chromatographic methods are relatively time consuming while ELISA may suffer from matrix interferences and leading to false‐positive results.…”