Comparison of Fenestra LC, ExiTron nano 6000, and ExiTron nano 12000 for Micro-CT Imaging of Liver and Spleen in Mice

Boll, Hanne; Figueiredo, Giovanna Negrão de; Fiebig, Teresa; Nittka, Stefanie; Doyon, Fabian; Kerl, H; Nölte, I.; Förster, Alex; Kramer, Martin; Brockmann, Marc A.

doi:10.1016/j.acra.2013.06.002

Cited by 44 publications

(64 citation statements)

References 21 publications

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“…Contrast was diluted 1:1 in sterile saline (50 μL contrast per animal, equivalent to ~640 mg iodine/kg body weight). Because this agent is taken up by cells of the reticuloendothelial system, it provides excellent contrast in liver and spleen lasting several weeks after injection (Supplemental Fig 1), and it has demonstrated low toxicity in small animal imaging (Boll et al, 2013; Boll et al, 2011). CT scans were performed at three different time points: pre-contrast, 2 h after contrast injection, and 2 weeks after capture.…”

Section: ) Materials and Methodsmentioning

confidence: 99%

Experimentally reducing corticosterone mitigates rapid captivity effects on behavior, but not body composition, in a wild bird

Lattin

Pechenenko

Carson

2017

Hormones and Behavior

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Wild animals and captives display physiological and behavioral differences, and it has been hypothesized, but rarely tested, that these differences are caused by sustained elevation of the hormone corticosterone. We used repeated computed tomography (CT) imaging to examine body composition changes in breeding male and female wild house sparrows (Passer domesticus; n=20) in response to two weeks of captivity, and assessed behavioral changes using video recordings. Half of the birds received the drug mitotane, which significantly decreased stress-induced corticosterone titers compared to controls. Based on the CT images, fat volumes increased, and pectoralis muscle density and heart and testes volumes decreased, over the two weeks of captivity in both groups of birds. However, beak-wiping, a behavior that can indicate anxiety and aggression, showed increased occurrence in controls compared to mitotane-treated birds. While our results do not support the hypothesis that these body composition changes were primarily driven by stress-induced corticosterone, our data suggest that experimentally reducing stress-induced corticosterone may mitigate some captivity-induced behavioral changes. Broadly, our results emphasize that researchers should take behavioral and physiological differences between free-living animals and captives into consideration when designing studies and interpreting results. Further, time in captivity should be minimized when birds will be reintroduced back to the wild.

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Section: ) Materials and Methodsmentioning

confidence: 99%

Experimentally reducing corticosterone mitigates rapid captivity effects on behavior, but not body composition, in a wild bird

Lattin

Pechenenko

Carson

2017

Hormones and Behavior

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“…To reduce skin doses, a 0.5 mm aluminium filter was used [29, 30]. Raw-data were reconstructed using a filtered back projection algorithm with a matrix of 512 x 512 x 512 (Reconstruction Studio, Tera Recon) [17, 18, 31, 32] and a smooth band-pass filter method, which we evaluated to provide the best soft tissue contrast. In-plane resolution was 40x40 μm.…”

Section: Methodsmentioning

confidence: 99%

Imaging of Orthotopic Glioblastoma Xenografts in Mice Using a Clinical CT Scanner: Comparison with Micro-CT and Histology

et al. 2016

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PurposeThere is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated.Materials and Methods2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i.v.) imaging using a micro-CT (80 kV, 75 μAs, 360° rotation, 1,000 projections, scan time 33 s, resolution 40 x 40 x 53 μm) and a clinical CT scanner (4-row multislice detector; 120 kV, 150 mAs, slice thickness 0.5 mm, feed rotation 0.5 mm, resolution 98 x 98 x 500 μm). Mice were sacrificed and the brain was worked up histologically. In all modalities tumor volume was measured by two independent readers. Contrast-to-noise ratio (CNR) and Signal-to-noise ratio (SNR) were measured from reconstructed CT-scans (0.5 mm slice thickness; n = 18).ResultsTumor volumes (mean±SD mm3) were similar between both CT-modalities (micro-CT: 19.8±19.0, clinical CT: 19.8±18.8; Wilcoxon signed-rank test p = 0.813). Moreover, between reader analyses for each modality showed excellent agreement as demonstrated by correlation analysis (Spearman-Rho >0.9; p<0.01 for all correlations). Histologically measured tumor volumes (11.0±11.2) were significantly smaller due to shrinkage artifacts (p<0.05). CNR and SNR were 2.1±1.0 and 1.1±0.04 for micro-CT and 23.1±24.0 and 1.9±0.7 for the clinical CTscanner, respectively.ConclusionClinical CT scanners may reliably be used for in vivo imaging and volumetric analysis of brain tumor growth in mice.

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“…Most currently commercially available MicroCT contrasts can be generally subdivided into particulate non-metabolizable macrophage specific and polydisperse metabolizable iodine-based contrasts 23,[33][34][35][36] . Although particulate agents offer greater X-ray opacification due to their higher atomic number (barium, Z=56; and gold, Z=79), they cannot be used for metabolic assessment.…”

Section: Discussionmentioning

confidence: 99%

<em>In Vivo</em> Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

Deel

Ridwan

Vliet

et al. 2016

JoVE

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The use of Micro-Computed Tomography (MicroCT) for in vivo studies of small animals as models of human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with the development of a novel preclinical iodinated contrast agent called eXIA160, functional and metabolic assessment of the heart became possible. However, prior to the advent of commercial MicroCT scanners equipped with X-ray flat-panel detector technology and easy-to-use cardio-respiratory gating, preclinical studies of cardiovascular disease (CVD) in small animals required a MicroCT technologist with advanced skills, and thus were impractical for widespread implementation. The goal of this work is to provide a practical guide to the use of the high-speed Quantum FX MicroCT system for comprehensive determination of myocardial global and regional function along with assessment of myocardial perfusion, metabolism and viability in healthy mice and in a cardiac ischemia mouse model induced by permanent occlusion of the left anterior descending coronary artery (LAD).

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Comparison of Fenestra LC, ExiTron nano 6000, and ExiTron nano 12000 for Micro-CT Imaging of Liver and Spleen in Mice

Cited by 44 publications

References 21 publications

Experimentally reducing corticosterone mitigates rapid captivity effects on behavior, but not body composition, in a wild bird

Experimentally reducing corticosterone mitigates rapid captivity effects on behavior, but not body composition, in a wild bird

Imaging of Orthotopic Glioblastoma Xenografts in Mice Using a Clinical CT Scanner: Comparison with Micro-CT and Histology

<em>In Vivo</em> Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

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