Comparison of fetal and adult human bone: Identification of a chemotactic factor in fetal bone

Somerman, Martha J.; Hotchkiss, R.N.; Bowers, Mary R.; Termine, John D.

doi:10.1016/0221-8747(83)90005-x

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…These results are similar to other studies which have shown that bone from younger animals is more inductive than that from older animals23 24 and the amount of chemotactic factor is different in fetal and adult human bone. 25 Contrary to donor age, gender had no effect on the ability of DFDBA to induce bone. Full expression of DFDBA induction ability in the nude mouse thigh muscle can be achieved if the DFDBA is implanted for 8 weeks.…”

Section: Discussionmentioning

confidence: 93%

Ability of Commercial Demineralized Freeze‐Dried Bone Allograft to Induce New Bone Formation Is Dependent on Donor Age But Not Gender

Schwartz

Somers

Mellonig

et al. 1998

Journal of Periodontology

215

166

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Demineralized freeze-dried bone allografts (DFDBA) have been used extensively in periodontal therapy. DFDBA is used because it contains bone morphogenetic protein (BMP), which induces new bone formation during the healing process. Most commercial bone banks do not verify the presence or activity of BMP in DFDBA nor the ability of DFDBA to induce new bone. Recently, we showed that different bone bank preparations of DFDBA, even from the same bank, varied considerably in their ability to induce new bone, suggesting inherent differences in the quality of the material. Therefore, we examined whether donor age or gender contributed to the variability seen with these preparations. Twenty-seven batches of DFDBA from different donors were donated by one bone bank which had been shown previously to supply DFDBA that was consistently able to induce new bone formation. Each batch was implanted bilaterally in the thigh muscle of nude mice. After 56 days, the implants were excised and examined by light microscopy and histomorphometry. Seventy percent of the preparations tested induced new bone formation. Most of these preparations produced ossicles containing cortical bone surrounding bone marrow-like tissue. The ability to induce bone appears to be age-dependent, with DFDBA from older donors being less likely to have strong bone-inducing activity. By contrast, no difference in ability to induce new bone was noticed between male or female donors. The results of this study confirm that commercial preparations of DFDBA differ in their ability to induce new bone formation. In fact, some of the batches had no activity at all. The ability of DFDBA to induce new bone formation is suggested to be age-dependent, but not gender-dependent by our study. These results indicate that commercial bone banks need to verify the ability of DFDBA to induce new bone formation and should reconsider the advisability of using bone from older donors.

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Section: Discussionmentioning

confidence: 93%

Ability of Commercial Demineralized Freeze‐Dried Bone Allograft to Induce New Bone Formation Is Dependent on Donor Age But Not Gender

Schwartz

Somers

Mellonig

et al. 1998

Journal of Periodontology

215

166

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“…Another long-standing debate exists over the use of fetal or adult tissues. Although there is evidence on special chemotactic agents in fetal human bone [26], the fetal bone marrow dynamics are quite different than the adult one. Using adult xenografts, a more precise recapitulation of the disease is attempted, not only in the context of WM, but also in the study of osteotropism of primary malignant tumors such as prostate and/or breast [21,30,31].…”

Section: Discussionmentioning

confidence: 96%

Monitoring Mast Cell Populations in Waldenström’s Macroglobulinemia: A Xenotransplantation Study

Siskos

Bekiari

Poutahidis

et al. 2019

JHOR

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Waldenström Macroglobulinemia (WM) is a B-cell lymphoproliferative disorder characterized mainly by uncontrolled accrual of M- immunoglobulin, secreted by malignant lymphoplasmatic cells. Mast cells interacting with malignant B-cells play an important role at the manifestation of the disease. Utilizing a previous xenotransplantation mouse model, this study evaluates long-term implant viability and quantifies distinct bone marrow mast cell populations along with their dynamics in non-WM and WM human bone implants. Non-WM bone implants were obtained from the femoral head of adult humans undergoing hip arthroplasty or hemiarthroplasty, whereas WM human bone implants originated from bone biopsies obtained from the posterior iliac crest of patients with active WM. All bone particles were implanted intramuscularly in twenty-four NOD/SCID mice. Following 3, 4 or 8 months postoperatively, xenografts were removed and studied using special histological techniques to identify mature and immature mast cells. Xenografts survived up to 8 months after implantation presenting normal cytoarchitecture (non-WM) or high-grade neoplastic infiltration and microresorption (WM bone biopsies). Statistical analysis of mast cell populations showed significant elevation regarding time progression and bone marrow microenvironment, thus suggesting the possible influence of malignant cells to the mast cell population in WM. This study presents the extended survival of intramuscular implantation of human adult bone xenografts into NOD/SCID mice and provides additional information on the interaction between mast cells and malignant B-cells.

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“…19 We have sought to further characterize proteins associated with ECM of bone and other mineralized tissues; e.g., cementum and dentin, and reported that protein extracts of mineralized tissues included proteins which enhanced attachment,20-21 protein production,22 and chemotactic activity of periodontal cells and gingival fibroblasts in vitro. [23][24] The studies described here focused on establishing biological activity of protein extracts prepared from commercially-obtained bone graft material. Furthermore, we were interested in determining whether these protein extracts possessed the same activity as that of protein extracts prepared from freshly obtained human bone.…”

Section: Commercially-prepared Auograft Materials Has Biological Activitymentioning

confidence: 99%

Commercially‐Prepared Allograft Material Has Biological Activity In Vitro

Shigeyama

D'Errico

Stone

et al. 1995

Journal of Periodontology

Self Cite

124

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The well-established finding that implantation of demineralized bone matrix at non-skeletal sites results in formation of cartilage and bone has been attributed to bone morphogenetic proteins/factors. Commercially-available demineralized bone allograft materials are being used currently to reconstruct/regenerate bone. The studies described here focused on establishing biological activity of protein extracts prepared from commercially obtained bone graft material in vitro. Furthermore, the biological activity of these protein extracts in vitro was compared with similar extracts prepared from freshly obtained human bone. Biological activities of bone matrix proteins examined included their ability to promote proliferation, attachment, and migration of gingival fibroblasts using an in vitro system. Guanidine followed by guanidine/EDTA was used to separate bone matrix proteins into proteins associated with soft tissues of bone and proteins retained within the mineral compartment, respectively. Two preparations of each starting material were tested and the biological activity of each preparation was evaluated in triplicate at least three times. Slot blot analysis revealed that commercially-prepared material contained type I collagen; fibronectin; BSP; and BMP-2, 4, and 7. However, the freshly prepared bone extracts appeared to have higher BMP concentrations. The ability of commercial extracts to promote cell proliferation, while significant, was limited and significantly less when compared with similar extracts prepared from freshly obtained bone. All extracts promoted cell attachment significantly, while none of the extracts promoted cell migration. Thus, commercially-prepared material retained proteins having the capacity to influence cell behavior in vivo. However, some biological activity as measured in vitro was lost as a result of tissue processing.

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Comparison of fetal and adult human bone: Identification of a chemotactic factor in fetal bone

Cited by 10 publications

References 12 publications

Ability of Commercial Demineralized Freeze‐Dried Bone Allograft to Induce New Bone Formation Is Dependent on Donor Age But Not Gender

Ability of Commercial Demineralized Freeze‐Dried Bone Allograft to Induce New Bone Formation Is Dependent on Donor Age But Not Gender

Monitoring Mast Cell Populations in Waldenström’s Macroglobulinemia: A Xenotransplantation Study

Commercially‐Prepared Allograft Material Has Biological Activity In Vitro

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