REFERENCES1. Kawasaki R, Wang JJ, Rochtchina E, Lee AJ, Wong TY, Mitchell P. Retinal vessel caliber is associated with the 10-year incidence of glaucoma: the Blue Mountains Eye Study. Ophthalmology 2013; 120: 84-90. 2. Sugiyama T, Araie M, Riva CE, Schmetterer L, Orgul S. Use of laser speckle flowgraphy in ocular blood flow research. Acta Ophthalmol (Copenh) 2010; 88: 723-9. 3. Aizawa N, Yokoyama Y, Chiba N et al. Reproducibility of retinal circulation measurements obtained using laser speckle flowgraphy-NAVI in patients with glaucoma. Clin Ophthalmol 2011; 5: 1171-6. 4. Aizawa N, Kunikata H, Yokoyama Y, Nakazawa T. Correlation between optic disc microcirculation in glaucoma measured with laser speckle flowgraphy and fluorescein angiography, and the correlation with mean deviation. Clin Experiment Ophthalmol 2014; 42: 293-4. 5. Shiga Y, Omodaka K, Kunikata H et al. Waveform analysis of ocular blood flow and the early detection of normal tension glaucoma. Inv Ophth Vis Sci 2013; 54: 7699-706.One-step reconstruction of large lower eyelid defects: technique and outcomesModerate to large lower eyelid defects following skin cancer excision or trauma present a challenge for the reconstructive surgeon. A myriad of techniques have been described in the literature; however, the goal remains to replace the missing anatomical layers and restore lower eyelid form and function. For the purpose of reconstruction, the lower eyelid is often divided into anterior and posterior lamellae: the posterior lamella, comprising tarsus and conjunctiva, provides protection to the eye by preventing conjunctival drying, providing a mechanical barrier to trauma and facilitating atraumatic eyelid closure with its lubricated, smooth surface. The anterior lamella, comprising skin and orbicularis oculi, provides an additional barrier and also facilitates eyelid movement. Large defects of the Competing/conflicts of interest: No stated conflict of interest. Funding sources: No stated funding sources.Figure 2. Box plot of vessel pixel count in the four study groups. Outlying values are included as small dots. The asterisks indicate statistically significant differences with normal eyes (Kruskal-Wallis test, followed by Steel-Dwass test; *P < 0.05, **P < 0.01).Figure 3. Box plot of area ratio of blood stream (ARBS) in the four study groups. Outlying values are included as small dots.The asterisks indicate statistically significant differences with normal eyes (Kruskal-Wallis test, followed by Steel-Dwass test; *P < 0.05; **P < 0.01).