Comparison of frequency distributions in flow cytometry

Cox, Christopher; Reeder, Jay E.; Robinson, R. D.; Suppes, Sarah B.; Wheeless, Leon L.

doi:10.1002/cyto.990090404

Cited by 49 publications

(45 citation statements)

References 9 publications

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“…Cumulative histograms were overlaid to establish the maximum absolute differences (D max ), and to reveal whether the differences between histograms are significant or not at p < 0.05 [6,24].…”

Section: Com) Mitotracker Deepmentioning

confidence: 99%

Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Nagy

Kakasi

Pál

et al. 2016

Acta Biologica Hungarica

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Local extreme climatic conditions occurring as a result of global climate change may interfere with the reproduction of animals. In the present study fish spermatozoa were incubated at different temperatures (20, 25, 30 and 40 °C) for 10 and 30 minutes, respectively and plasma membrane integrity and mitochondrial membrane potential changes were evaluated with flow cytometry using SYBR-14/PI and Mitotracker Deep Red FM fluorescent dyes. No significant differences were found in plasma membrane integrity at either incubation temperatures or time points. Mitotracker Deep Red FM histogram profiles indicating mitochondrial activity showed significant (p < 0.001) alterations in all cases of higher (25, 30 and 40 °C) temperature treatments as compared to the samples incubated at 20 °C. Our results indicate that fish spermatozoa exposed to high temperatures suffer sublethal damage that cannot be detected with conventional, vital staining techniques.

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Section: Com) Mitotracker Deepmentioning

confidence: 99%

Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Nagy

Kakasi

Pál

et al. 2016

Acta Biologica Hungarica

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“…The Kolmogorov-Smirnov test was applied to calculate the D-value (ie, the maximal difference between the test and its negative control). 12 On the basis of expression in normal peripheral blood cells and bone marrow cells, cases with a D-value of >0.3 were considered to be overexpressing ABCG2.…”

Section: Abcg2 Expressionmentioning

confidence: 99%

Concomitant ABCG2 overexpression and FLT3‐ITD mutation identify a subset of acute myeloid leukemia patients at high risk of relapse

Tiribelli¹,

Geromin²,

Michelutti³

et al. 2010

Cancer

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BACKGROUND: ABCG2 protein overexpression and FLT3 internal tandem duplication (ITD) correlate with higher relapse rate and shorter disease-free survival (DFS) in acute myeloid leukemia (AML), but no data are available on the possible effect of concomitant presence of these 2 factors. METHODS: The authors analyzed the outcome of 166 cases of adult AML patients who were homogeneously treated with a fludarabine-based induction therapy. RESULTS: ABCG2 overexpression and FLT3-ITD were detected in 83 (50%) and 47 (28%) patients, respectively. A significant correlation was found between ABCG2 positivity and FLT3 mutation, with 33 (40%) ITD in 83 ABCG2-positive patients compared with 14 (17%) ITD in 83 ABCG2-negative patients (P ¼ .002). Complete remission (CR) after induction therapy was achieved in 95 (57%) patients. Neither ABCG2 overexpression nor FLT3-ITD had any impact on achievement of CR. Relapse occurred in 42 of 95 (44%) patients at a median time of 28 months. Time to relapse was shortened in patients overexpressing ABCG2 (P ¼ .0004). DFS was not affected by FLT3-ITD alone, but FLT3 mutation significantly worsened long-term outcome of ABCG2-positive patients. DFS at 1 and 3 years in patients with overexpression of both ABCG2 and FLT3-ITD was only 36% and 28%, respectively; in ABCG2-positive/FLT3-negative patients, DFS at 1 and 3 years was 65% and 48%, respectively; and in ABCG2-negative cases (regardless of FLT3 status), DFS at 1 and 3 years was greater than 85% and 75%. CONCLUSIONS: Concomitant overexpression of ABCG2 and FLT3-ITD is relatively frequent and identifies a subgroup of AML patients with a significantly worse prognosis. The possible interactions between these 2 prognostic factors need to be defined. Cancer 2011;117:2156-62.

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“…The increase (1)) MDA-MB-468 cells (23). According to the probability binning (PB) comparison algorithm, the two cell population distributions were different with 99% confidence (P \ 0.01, T(v) 5 2,812), demonstrating statistical significance (24,25). The cell population distributions as observed by the 530 channel were indistinguishable (P 5 0.5, T(v) 5 0), confirming that use of a spectral channel matched to the nanorod excitation peak was essential in observing the labeling.…”

Section: Resultsmentioning

confidence: 99%

Plasmonic flow cytometry by immunolabeled nanorods

Crow¹,

Marinakos²,

Cook³

et al. 2010

Cytometry Pt A

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Fluorescence-based flow cytometry measures multiple cellular characteristics, including levels of receptor expression, by assessing the fluorescence intensity from a population of cells whose cell surface receptors are bound by a fluorescently labeled antibody or ligand for that receptor. Functionalized noble metal nanoparticles provide a complementary method of receptor labeling based on plasmonics for population analysis by flow cytometry. The potential benefits of using plasmonic nanoparticles to label cell surface receptors in flow cytometry include scattering intensity from a single particle that is equivalent to fluorescence intensity of 10 5 fluorescein molecules, biocompatibility and low cytotoxicity, and nonquenching optical properties. The large spectral tunability of nanorods also provides convenient access to plasmonic markers with peak surface plasmon resonances ranging from 600 to 2,200 nm, unlike gold nanosphere markers that are limited to visible wavelengths. Gold nanorod-based plasmonic flow cytometry is demonstrated herein by comparing the scattering of cells bound to antiepidermal growth factor receptor (EGFR)-conjugated nanorods to the emission of cells bound to anti-EGFR-conjugated fluorescent labels. EGFR-expressing cells exhibited a statistically significant six-fold increase in scattering when labeled with anti-EGFRconjugated nanorods compared with labeling with IgG1-conjugated nanorods. Large scattering intensities were observed despite using a 1,000-fold lower concentration of nanorod-conjugated antibody relative to the fluorescently labeled antibody. ' 2010International Society for Advancement of Cytometry Key terms flow cytometry; nanorod; plasmonic markers; epidermal growth factor receptor NOBLE metal nanoparticles exhibit unique optical properties that can be exploited for a variety of applications. These properties arise from the interaction of an incident electromagnetic (EM) field with free conduction band electrons in the nanoparticle. The conduction electrons are polarized relative to the heavier positive core ions because of the exciting EM field (1). The charge separation results in a restoring force and subsequent coherent oscillation of the conduction band electrons, sharply enhancing scattering and absorption at specific resonance frequencies. The scattering spectra of nanoparticles are dictated by intrinsic parameters such as their size, shape, and composition, as well as extrinsic factors such as the local refractive index of the surrounding medium. These intrinsic parameters enable the design of gold nanoparticles with peak resonances that can be tuned to specific wavelengths across a relatively wide spectral region (600-2,200 nm) simply by modifying the synthesis protocol to realize the appropriate structural parameters (2,3). Because slight changes in the local refractive index of nanoparticles result in a peak wavelength shift, noble metal nanoparticles are also exquisitely sensitive reporters of their local, nanoscale environment.The unique properties of p...

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Comparison of frequency distributions in flow cytometry

Cited by 49 publications

References 9 publications

Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Concomitant ABCG2 overexpression and FLT3‐ITD mutation identify a subset of acute myeloid leukemia patients at high risk of relapse

Plasmonic flow cytometry by immunolabeled nanorods

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