Nutritional composition, functional and antioxidant properties of different parts from grass turtle were investigated. Muscle contained high protein, essential amino acids, and vitamin B6, which were 74.97%, 28363.84, and 14.47 mg/100 g, respectively. Unsaturated fatty acids, vitamin K were significantly higher in the liver which reached 7489.45 and 1591.67 mg/100 g, respectively. Hardshell high content of minerals and vitamin B3 (48998.06 and 17.52 mg/100 g), correspondingly. The molecular weight was obtained in the range of 0.27-87.38% (Mw˂1000 Da). Solubility, emulsifying activity, and emulsion stability index at different pH ranged from 3.89 to 45.89%, 47.53 to 90.22 m 2 /g, and 65.45 to 89.93%, respectively. Water and oil holding capacities were high in muscle (7.97 and 2.19 mL/g). The highest antioxidant activity was observed in the muscle (88.58% ABTS) and liver (66.71% DPPH and 0.49 FRAP). Muscle, liver, and hard shell properties will provide producers and consumers with useful new information about the nutritional value and health benefits.