Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp

Zhou, Lei; Han, Yu; Wei, Dapeng; Li, Yusen; Huang, Xiande; He, Anyou

doi:10.1007/s00343-020-0214-3

Cited by 11 publications

(22 citation statements)

References 48 publications

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“…However, in the present study, signi cant differences were observed only between the fore-and mid-intestine of L. calcarifer, and between the three different intestinal segments of T. blochii. While no remarkable differences were observed for fungal community in other segments of the intestine, which was comparable to the ndings for bighead carp where there were no remarkable differences in fungi between intestinal segments (Zhou et al 2021). Among the intestinal fungi, we found a dramatic decrease in the number of yeasts in the hind-intestine in comparison to the fore-and mid-intestine, in addition to differences in the distribution of Trichoderma spp.…”

Section: Comparison Of Fungal In Environments and Intestines From The...supporting

confidence: 85%

“…and Talaromyces spp.. In a survey of tilapia and bighead carp, it was also found that the hind-intestines showed a signi cant reduction in pathogenicity and saprotrophicity compared with the fore-intestine (Zhou et al 2021). These results indicated that the distribution of the fungi in the sh intestine is associated with the physiological functions of various intestinal segments except for the speci city of the host species.…”

Section: Comparison Of Fungal In Environments and Intestines From The...mentioning

confidence: 96%

“…As a diversi ed and widespread group of eukaryotes, fungi are major decomposers of microorganisms (Zhou et al 2021). They should be regarded as essential parts of the intestinal microbiota due to their ability to maintain the normal functioning of physiological processes and tissue health and promote the proper progression of the immune system (Underhill and Iliev 2014).…”

Section: )mentioning

confidence: 99%

“…As fungi derived from marine origins, Cladosporium and Trichoderma species are widely found in sediment, seagrass, sponges, and different sh species (Silphaduang et al 2000; Panno et al 2013; Yu et al 2013; Zhang et al 2018; Meyers et al 2019; Cohen et al 2021; Zou et al 2021).Yet, Aureobasidium is a black yeast-like fungus, and some strains produce various polysaccharides, including β-glucan, which are known to exert immunostimulatory effects (Tanioka et al 2013). It is present in the intestinal tract of many freshwater sh as symbiotrophic fungi, such tilapia (Oreochromis mossambicus) and bighead carp (Aristichthys nobilis)(Zhou et al 2021). And Aureobasidium had also been found in the intestine of marine sh.…”

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confidence: 99%

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Biodiversity and antimicrobial activity of intestinal fungi from three species of coral reef fishes

Chen

Zeng

et al. 2022

Preprint

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Although intestinal microbiota plays crucial roles in fish digestion and health, little is known about intestinal fungi in fishes. This study investigated the intestinal fungal diversity of three coral reef fishes (Lates calcarifer, Trachinotus blochii and Lutjanus argentimaculatus) from the South China Sea using a culturable method. A total of 387 isolates were recovered and identified by sequencing their internal transcribed spacer sequences, belonging to 29 known fungal species. The similarity of fungal community in intestines of the three fishes verified the fungal colonization may be influenced by their surrounding environments. Furthermore, the fungal communities in different intestines of some fishes were significantly different, and the number of yeasts in hindgut was less than that in fore- and mid-intestine, suggesting that the distribution of fungi in fishes intestines may be related to the physiological functions of various intestinal segment. In addition, 51.4% tested fungal isolates exhibited antimicrobial activity against at least one marine pathogenic microorganisms. Notably, isolate Aureobasidium pullulans SCAU243 exhibited a strong antifungal activity against Aspergillus versicolor, and isolate Schizophyllum commune SCAU255 displayed extensive antimicrobial activity against four marine pathogenic microorganisms. This study contributed to our understanding of intestinal fungi in coral reef fish and further increasing the library of fungi available for natural bioactive product screening.

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Section: Comparison Of Fungal In Environments and Intestines From The...supporting

confidence: 85%

Section: Comparison Of Fungal In Environments and Intestines From The...mentioning

confidence: 96%

Section: )mentioning

confidence: 99%

mentioning

confidence: 99%

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Biodiversity and antimicrobial activity of intestinal fungi from three species of coral reef fishes

Chen

Zeng

et al. 2022

Preprint

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“…We also detected Ascomycota in low abundance in two samples of the control group, but this taxon was not detected in the M-primers group. These three fungal phyla were recently documented as being part of fish commensal mycobiome in tilapia and bighead carp (Zhou et al . 2021).…”

Section: Festivus Eukaryotic Microbiomementioning

confidence: 99%

Elongation arrest blocking primers enhance parasite detection in 18S rRNA metabarcoding study of Mesonauta festivus gut eukaryotic communities.

Leroux

Bouslama

Sylvain

et al. 2022

Preprint

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The study of the eukaryotic fraction of the microbiota using a metabarcoding approach is usually hindered by the high host to eukaryotic microbiota DNA ratio in samples. Indeed, the 18S rRNA gene is very similar for both the host and its eukaryotic communities, leading to a preferential amplification of the predominant host DNA when using universal primers. Multiple approaches have been developed to reduce host DNA amplification. One method is based on elongation arrest blocking primers, oligonucleotides modified with a C3 Spacer that stops the advancement of the DNA polymerase at non-conserved regions of a target gene. In this paper, we successfully developed and tested species-specific elongation arrest blocking primers to block the Flag cichlid, Mesonauta festivus, 18S rRNA SSU. Our elongation arrest blocking primers significantly reduced the amount of host DNA in samples by 66 %. In addition to reducing the amount of sequencing wasted, the blocking primers increased the detectability of potentially dangerous parasitic taxa in fish gut, highlighting the potential of the method for parasitic screening. For instance, we discovered a case of infection by the parasitic ciliate Nyctotherus sp. Also, we detected the presence of a parasitic Trematode and an Amoebae, collected compelling data on the species feeding habits and obtained data on the commensal eukaryotic diversity present in M. festivus gut. While our data support the possibility of achieving a complete inhibition of host DNA amplification using elongation arrest blocking primers, more research is still required. Still, there is a need for the development and additional testing of protocols to study the eukaryotic diversity present in fish gut, a slow-growing field of study in comparison to its prokaryotic counterpart.

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Connection between the gut microbiota of largemouth bass (Micropterus salmoides) and microbiota of the aquaponics system environment

Yang,

Li,

et al. 2024

Environ Sci Pollut Res

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Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp

Cited by 11 publications

References 48 publications

Biodiversity and antimicrobial activity of intestinal fungi from three species of coral reef fishes

Biodiversity and antimicrobial activity of intestinal fungi from three species of coral reef fishes

Elongation arrest blocking primers enhance parasite detection in 18S rRNA metabarcoding study of Mesonauta festivus gut eukaryotic communities.

Connection between the gut microbiota of largemouth bass (Micropterus salmoides) and microbiota of the aquaponics system environment

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