Comparison of ganglioside expression between human adipose- and dental pulp-derived stem cell differentiation into osteoblasts

Abstract

Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoietic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast-differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.

Stem Cell Glycolipids

“…In human mesenchymal stem cells from dental papilla, GM3, GM2, and GD1a, but not GM1, were biochemically detected [82]. The expression of these gangliosides was also found in human dental pulp-derived stem cells [75] and adipose-derived stem cells [83]. The expression patterns of these a-series gangliosides in mesenchymal stem cells were found to change during their neuronal or osteoblast differentiation [51,75,82,83].…”

“…In mesenchymal stem cells isolated from human periodontal ligament, SSEA-4 and SSEA-1 have been found by flow cytometric and immunocytochemical analyses [88]. Nevertheless, GD2 and SSEA-4 have not been biochemically detected in ganglioside fractions extracted from mesenchymal stem cells in bone marrow, dental papilla, dental pulp, and adipose [51,75,82,83]. These gangliosides may be tissue type-specifically expressed or quite minor components in mesenchymal stem cells.…”

Histochemical examination of adipose derived stem cells combined with β-TCP for bone defects restoration under systemic administration of 1α,25(OH) 2 D 3

“…Multilocular adipocytes are considered intermediate adipocytic cell types between preadipocytes and mature unilocular adipocytes [12,32]. The ADSCs express the mesenchymal stem cell specific surface antigens, CD13, CD29, CD44, CD73, CD90, CD105, CD133 and CD166, but negative for hematopoietic markers, CD14, CD11b, CD34, CD45, CD19 CD79 and CD117 [12,33]. In progressive osseous heteroplasia, a rare pathological disorder, subcutaneous extramedullary fat differentiates into ectopic bone, but the underlying etiology remains unknown [34].…”

Differential expression pattern of gangliosides during the differentiation of human dental pulp-derived mesenchymal stem cells into dopaminergic neural-like cells

“…Previously, we detected GM3, GM2, and GD1a in hMSCs (Kim et al 2008;Ryu et al 2009;Lee et al 2010;Yang et al 2011). …”

“…The morphology of established hDPSCs was fibroblastic (Supplementary Figure 1A). 1 Previous studies involving FACS analysis established that hDPSCs have human mesenchymal stem cell (hMSC) characteristics (Ryu et al 2009;Lee et al 2010). Specifically, the cells expressed the MSC-specific surface antigens CD44 and CD105, but not the hematopoietic markers CD45 and CD117 (Supplementary Figure 1B).…”