Histochemical examination of adipose derived stem cells combined with β-TCP for bone defects restoration under systemic administration of 1α,25(OH) 2 D 3

Feng, Wei; Lv, Shengyu; Cui, Jian; Han, Xiuchun; Du, Juan; Sun, Jing; Wang, Kefeng; Wang, Zhenming; Li, Xiong; Guo, Jie; Oda, Kimimitsu; Amizuka, Norio; Xu, Xin; Li, Minqi

doi:10.1016/j.msec.2015.05.037

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…[34][35][36] In several in vivo studies, ADSCs were used to reconstruct critical sized bone defects. [37][38][39][40] Considering the fact that most studies used rodent cells and/or focused on global effects of coculturing ADSCs and endothelial cells, we pursued a translational approach using human primary cells and described cell type specific effects upon coculturing using negative immunoselection. In addition to that, we tried to determine the ideal cell ratio of ADSCs and HUVECs with regard to proliferation, apoptosis, osteogenic differentiation and angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Bone tissue engineering using adipose‐derived stem cells and endothelial cells: Effects of the cell ratio

Mutschall

Winkler

Weisbach

et al. 2020

J Cellular Molecular Medi

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The reconstruction of critical sized bone defects can be challenging in clinical practice. Critical bone defects can be caused by malformation, cancer, trauma or infection. Regardless of the entity, the current gold standard is autologous tissue transfer, which can be associated with significant donor side morbidity and limited tissue availability. One way to circumvent these problems is the generation of bioartificial bone tissue.For bone formation and regeneration, a sufficient vascularization providing oxygen and nutrition supply is indispensable. [1][2][3] Strategies to improve vascularization in bone tissue engineering applications include the use of angiogenic growth factors, endothelial cells (ECs) and the surgical induced angiogenesis by means of arteriovenous

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Section: Discussionmentioning

confidence: 99%

Bone tissue engineering using adipose‐derived stem cells and endothelial cells: Effects of the cell ratio

Mutschall

Winkler

Weisbach

et al. 2020

J Cellular Molecular Medi

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“…Among the scaffolds, the implants bearing similarity to native bone composition including β-tricalcium phosphate, hydroxyapatite, and type I collagen have been reported to be effective in the repair of bone defects [44–48]. Moreover, the implantation of a scaffold-cell combination has demonstrated better osteogenic capacity that that obtained when the scaffolds were co-transplanted with MSCs [20,37,39,49,50]. In transplanting MSCs, important factors include whether the cells can be transported into the surgical site and whether the implanted cells can be functionally engrafted therein.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cell sheets accelerate bone healing in rat femoral defects

et al. 2019

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In the present study, we investigated whether both adipose-derived stem cell (ADSC) and osteogenic-induced ADSC sheets could promote bone healing in a rat distal femoral metaphysis bone defect model. A through-hole defect of 1 mm diameter was drilled into each distal femur of 12 week old rats. Forty-five rats were randomly assigned to three groups: (1) control group; (2) ADSC sheet group; or (3) osteogenic-induced ADSC sheet group. We evaluated each group by analysis of computerized tomography scans every week after the surgery, histological analysis, and DiI labeling (a method of membrane staining for post implant cell tracing). Radiological and histological evaluations showed that a part of the hole persisted in the control group at four weeks after surgery, whereas the hole was restored almost completely by new bone formation in both sheet groups. The mean value of bone density (in Houndsfield units) for the bone defect area was significantly higher in both sheet groups than that in the control group ( p = 0.05) at four weeks postoperative. A large number of osteocalcin positive osteoblasts were observed at the area of bone defect, especially in the osteogenic-induced ADCS sheet group. DiI labeling in the newly formed bone showed that each sheet had differentiated into bone tissue at four weeks after surgery. The ADSC and the osteogenic-induced ADSC sheets promoted significantly quicker bone healing in the bone defect. Moreover, the osteogenic-induced ADSC sheet may be more advantageous for bone healing than the ADSC sheet because of the higher number of osteocalcin positive osteoblasts via the transplantation.

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“…6,7 Higher performance silicone rubber requires vulcanization process because this process could build highly three dimensional networks in the materials, making them more tougher than linear PDMS. [2][3][4][5][6][8][9][10][11][12][13][14][15][16] Vulcanization of silicone rubber can be carried out at both elevated and ambient temperatures, and the silicone rubber obtained is called high temperature vulcanization (HTV) silicone rubber and room temperature vulcanization (RTV) silicone rubber, respectively. 6,8,16 Although RTV silicon rubber have poorer mechanical properties compared to HTV silicones, they are easier to make and hence widely used.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of room temperature vulcanized silicone rubber using α-amine ketoximesilanes as auto-catalyzed cross-linkers

Zhao

Liu

et al. 2016

RSC Adv.

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Histochemical examination of adipose derived stem cells combined with β-TCP for bone defects restoration under systemic administration of 1α,25(OH) 2 D 3

Cited by 10 publications

References 40 publications

Bone tissue engineering using adipose‐derived stem cells and endothelial cells: Effects of the cell ratio

Bone tissue engineering using adipose‐derived stem cells and endothelial cells: Effects of the cell ratio

Adipose-derived stem cell sheets accelerate bone healing in rat femoral defects

Preparation and characterization of room temperature vulcanized silicone rubber using α-amine ketoximesilanes as auto-catalyzed cross-linkers

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