Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles

Kostrominova, Tatiana Y.; Dow, Douglas E.; Dennis, Robert G.; Miller, Richard A.; Faulkner, John A.

doi:10.1152/physiolgenomics.00210.2004

Cited by 70 publications

(61 citation statements)

References 99 publications

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“…Consistent with mTORC1 activation, we observed that protein synthesis is up-regulated in denervated muscles using the recently developed SUnSET method; this finding supports previously published observations using radioisotope-labeled amino acids (10,11). Furthermore, it has been shown that denervation increases the mRNA expression of a wide range of genes involved in protein translation such as ribosomal subunits and translation initiation and elongation factors (2,37,43). Thus, our study confirms that denervated muscles show both catabolic and anabolic features.…”

Section: Discussionsupporting

confidence: 85%

“…mTORC1 activation can also stimulate ribosome biogenesis through translation of ribosome proteins and transcription of rRNA genes (27,36). Indeed, it has been reported that transcripts of ribosomal subunits are increased in denervated muscles (2,37). We confirmed that the amount of ribosomal proteins S6 and L7, members of the 40 S and 60 S ribosomal subunit, were increased by 80 and 50%, respectively, in denervated muscles at 2 days post-operation (Fig.…”

Section: Mtorc1 Activation By Denervationsupporting

confidence: 76%

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Proteasome-dependent Activation of Mammalian Target of Rapamycin Complex 1 (mTORC1) Is Essential for Autophagy Suppression and Muscle Remodeling Following Denervation

Quy

Kuma

Pierre

et al. 2013

Journal of Biological Chemistry

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Background:The overall protein metabolism during denervation atrophy remains unclear. Results: Autophagy is suppressed, whereas ribosome and protein synthesis are up-regulated in denervated muscles by proteasome-dependent activation of mTORC1. Conclusion: Denervation does not simply induce muscle degradation but also promotes proteasome-and mTORC1-dependent muscle remodeling. Significance: This information is beneficial for understanding the pathophysiology of other types of muscle atrophy.

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Section: Discussionsupporting

confidence: 85%

Section: Mtorc1 Activation By Denervationsupporting

confidence: 76%

Proteasome-dependent Activation of Mammalian Target of Rapamycin Complex 1 (mTORC1) Is Essential for Autophagy Suppression and Muscle Remodeling Following Denervation

Quy

Kuma

Pierre

et al. 2013

Journal of Biological Chemistry

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“…3,16,17 Genes inhibiting cell cycle progression were significantly downregulated in rat gastrocnemius muscle 1 month after denervation, with return to baseline in muscle denervated for 3 months. 3 It was suggested that this pattern could result in activation and proliferation of muscle satellite cells.…”

Section: Discussionmentioning

confidence: 99%

“…3 Similarly, rat extensor digitorum longus muscle showed upregulation of NCAM and other developmentally and neurally regulated molecules in muscle denervated for 2 months. 16 Moreover, immunofluorescence demonstrated that these molecules were expressed by small fibers at the protein level. 16 We reject the hypothesis that these small fibers are the result of denervation.…”

Section: Discussionmentioning

confidence: 99%

“…16 Moreover, immunofluorescence demonstrated that these molecules were expressed by small fibers at the protein level. 16 We reject the hypothesis that these small fibers are the result of denervation. In the first place, denervated fibers showing as little cytoplasm as those depicted in Figures 1 and 2 are expected to contain abundant myonuclei, resulting in "nuclear clumps."…”

Section: Discussionmentioning

confidence: 99%

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Myogenesis in human denervated muscle biopsies

2007

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Electron microscopic studies of long-term denervated rat muscles have identified very small, immature myofibers that are believed to arise from detached satellite cells that have fused to form new fibers within the interstitial space. At present, it is unknown whether and to what extent equivalent fibers exist in denervated human muscle. Serial sections of muscle biopsies from 66 patients diagnosed with polyneuropathy or amyotrophic lateral sclerosis were immunolabeled with anti-NCAM and antineonatal myosin heavy chain monoclonal antibodies that are both neurally and developmentally regulated. We evaluated 200 myofibers in each section. Of the biopsy specimens, 75% contained small myofibers that showed a thin perinuclear cytoplasmic rim. Small fibers expressing neonatal myosin heavy chain (MHCnϩ) were found in all of these biopsies (100%) and NCAMϩ fibers in 98%. The percentage of MHCnϩ small fibers averaged 82% and NCAMϩ small myofibers averaged 40%. The percentage of NCAMϩ small fibers was significantly lower than that of MHCnϩ fibers. In contrast, the percentage of MHCnϩ vs. NCAMϩ angular atrophic fibers did not show a significant difference. A substantial subset of neurogenic biopsies showed small fibers that differ from angular atrophic fibers both in size and expression pattern of MHCn and NCAM. Myogenesis appears to be a frequent finding in neurogenic atrophy.

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Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies

Kostrominova

2010

Microscopy Res & Technique

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During immunostaining of specific proteins in tissue sections using monoclonal and polyclonal antibodies visualization of general tissue staining/background or major structural features is helpful to pinpoint precise localization of the protein of interest. Often in skeletal muscle research immunostaining with antibodies against connective tissue or plasma membrane proteins (collagen 1, laminin, caveolin 3) are used for this purpose. Although immunostaining for these proteins works well, it is time consuming, costly, limits the number of antibodies against protein of interest that can be used on a single section, and is not applicable to some staining techniques. Lectins were frequently used in earlier publications for skeletal muscle fiber boundaries and connective tissue visualization, but are not common in the current research studies. The present paper investigates co-staining of muscle, bone, ligament and tendon tissue sections with fluorescently tagged wheat germ agglutinin (WGA) lectin as a tool for visualization of connective tissue. The results of the current study show that fluorescent WGA lectin co-staining is a cost-effective, fast and convenient method for connective tissue visualization, especially in the studies where extensive washes reduce staining of the structures that are the primary interest of the investigation.

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Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles

Abstract: . Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.

Cited by 70 publications

References 99 publications

Proteasome-dependent Activation of Mammalian Target of Rapamycin Complex 1 (mTORC1) Is Essential for Autophagy Suppression and Muscle Remodeling Following Denervation

Proteasome-dependent Activation of Mammalian Target of Rapamycin Complex 1 (mTORC1) Is Essential for Autophagy Suppression and Muscle Remodeling Following Denervation

Myogenesis in human denervated muscle biopsies

Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies

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