Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

Wang, Bing; Li, Fuhua; Luan, Weiling; Xie, Yusu; Zhang, Chengsong; Luo, Zhan; Liu, Gui; Yan, Hui; Xiang, Jianhai

doi:10.1007/s10126-008-9105-x

Cited by 49 publications

(10 citation statements)

References 49 publications

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“…Thus, WSSV and YHV viral infections affect the expression of a 4.6-and 2.7-fold, respectively, higher number of shrimp genes than that induced by V. harveyi bacterial infection. This finding is in accordance with the study by Wang et al [9] who reported that the number of WSSV responsive genes in the Chinese shrimp, Fenneropenaeus chinensis, was 1.7-fold higher than that found in Vibrio anguilarum infected shrimps. Comparison of the number of responsive genes between WSSV and YHV infected shrimps revealed a 1.7-fold higher number for WSSV infection.…”

Section: Microarray Analysis Of Differentially Expressed Genes In Patsupporting

confidence: 95%

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

Pongsomboon

Tang

Boonda

et al. 2011

Fish & Shellfish Immunology

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Section: Microarray Analysis Of Differentially Expressed Genes In Patsupporting

confidence: 95%

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

Pongsomboon

Tang

Boonda

et al. 2011

Fish & Shellfish Immunology

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“…However, few reports suggest that FABPs is associated with immunity [1]. For example, differential expression of L-FABP and H-FABP was observed in Fenneropenaeus chinensis challenged with white spot syndrome virus (WSSV) and Vibrio [26]. Suppression subtractive hybridization results showed upregulated expression of FABP in the hepatopancreas of WSSV resistant and susceptible shrimp innate immunity [27].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Binding Proteins FABP9 and FABP10 Participate in Antibacterial Responses in Chinese Mitten Crab, Eriocheir sinensis

Lin¹,

Jin²,

Li³

et al. 2013

PLoS ONE

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Invertebrates rely solely on the innate immune system for defense against pathogens and other stimuli. Fatty acid binding proteins (FABP), members of the lipid binding proteins superfamily, play a crucial role in fatty acid transport and lipid metabolism and are also involved in gene expression induced by fatty acids. In the vertebrate immune system, FABP is involved in inflammation regulated by fatty acids through its interaction with peroxidase proliferator activate receptors (PPARs). However, the immune functions of FABP in invertebrates are not well characterized. For this reason, we investigated the immune functionality of two fatty acid binding proteins, Es-FABP9 and Es-FABP10, following lipopolysaccharide (LPS) challenge in the Chinese mitten crab (Eriocheir sinensis). An obvious variation in the expression of Es-FABP9 and Es-FABP10 mRNA in E. sinensis was observed in hepatopancreas, gills, and hemocytes post-LPS challenge. Recombinant proteins rEs-FABP9 and rEs-FABP10 exhibited distinct bacterial binding activity and bacterial agglutination activity against Escherichia coli and Staphylococcus aureus. Furthermore, bacterial growth inhibition assays demonstrated that rEs-FABP9 responds positively to the growth inhibition of Vibrio parahaemolyticuss and S. aureus, while rEs-FABP10 responds positively to the growth inhibition of Aeromonas hydrophila and Bacillus subtilis. Coating of agarose beads with recombinant rEs-FABP9 and rEs-FABP10 dramatically enhanced encapsulation of the beads by crab hemocytes in vitro. In conclusion, the data presented here demonstrate the participation of these two lipid metabolism-related proteins in the innate immune system of E. sinensis.

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“…Thus far, the response of shrimps upon Vibrio infection has been reported only at the transcriptional level [5-7]. Indeed, several groups of V. harveyi -responsive genes in shrimps have been identified.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection

et al. 2010

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BackgroundViral and bacterial diseases can cause mass mortalities in commercial shrimp aquaculture. In contrast to studies on the antiviral response, the responses of shrimps to bacterial infections by high throughput techniques have been reported only at the transcriptional level and not at the translational level. In this study, a proteomic analysis of shrimp hemocytes to identify differentially expressed proteins in response to a luminous bacterium Vibrio harveyi was evaluated for its feasibility and is reported for the first time.ResultsThe two-dimensional gel electrophoresis (2-DE) patterns of the hemocyte proteins from the unchallenged and V. harveyi challenged shrimp, Penaeus monodon, at 24 and 48 h post infection were compared. From this, 27 differentially expressed protein spots, and a further 12 weakly to non-differentially regulated control spots, were selected for further analyses by the LC-ESI-MS/MS. The 21 differentially expressed proteins that could be identified by homologous annotation were comprised of proteins that are directly involved in the host defense responses, such as hemocyanin, prophenoloxidase, serine proteinase-like protein, heat shock protein 90 and alpha-2-macroglobulin, and those involved in signal transduction, such as the14-3-3 protein epsilon and calmodulin. Western blot analysis confirmed the up-regulation of hemocyanin expression upon bacterial infection. The expression of the selected proteins which were the representatives of the down-regulated proteins (the 14-3-3 protein epsilon and alpha-2-macroglobulin) and of the up-regulated proteins (hemocyanin) was further assessed at the transcription level using real-time RT-PCR.ConclusionsThis work suggests the usefulness of a proteomic approach to the study of shrimp immunity and revealed hemocyte proteins whose expression were up regulated upon V. harveyi infection such as hemocyanin, arginine kinase and down regulated such as alpha-2-macroglobulin, calmodulin and 14-3-3 protein epsilon. The information is useful for understanding the immune system of shrimp against pathogenic bacteria.

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Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

Cited by 49 publications

References 49 publications

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

Fatty Acid Binding Proteins FABP9 and FABP10 Participate in Antibacterial Responses in Chinese Mitten Crab, Eriocheir sinensis

Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection

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