Comparison of genetic transformation in Morus alba L. via different regeneration systems

Agarwal, Swapnil; Kanwar, Kamlesh

doi:10.1007/s00299-006-0217-3

Cited by 35 publications

(15 citation statements)

References 12 publications

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“…The osmotin gene construct was introduced into mulberry by Agrobacterium-mediated transformation using different mulberry explants and regenerative callus tissue. A number of prior reports describe the use of regenerative callus for the transformation in mulberry (Nozue et al 2000;Aggarwal and Kanwar 2006), but the efficiency of transformation was low in these studies. In contrast, in our Inducible expression by the rd29A promoter…”

Section: Osmotin Overexpression By the Camv 35s Promotermentioning

confidence: 91%

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

et al. 2010

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Osmotin and osmotin-like proteins are stress proteins belonging to the plant PR-5 group of proteins induced in several plant species in response to various types of biotic and abiotic stresses. We report here the overexpression of tobacco osmotin in transgenic mulberry plants under the control of a constitutive promoter (CaMV 35S) as well as a stress-inducible rd29A promoter. Southern analysis of the transgenic plants revealed the stable integration of the introduced genes in the transformants. Real-time PCR analysis provided evidence for the expression of osmotin in the transgenic plants under both the constitutive and stress-inducible promoters. Transgenic plants with the stress-inducible promoter were observed to better tolerate salt and drought stress than those with the constitutive promoter. Transgenic plants when subjected to simulated salinity and drought stress conditions showed better cellular membrane stability (CMS) and photosynthetic yield than non-transgenic plants under conditions of both salinity and drought stress. Proline levels were very high in transgenic plants with the constitutive promoter relative to those with the stress-inducible promoter. Fungal challenge undertaken with three fungal species known to cause serious losses to mulberry cultivation, namely, Fusarium pallidoroseum, Colletotrichum gloeosporioides and Colletotrichum dematium, revealed that transgenic plants with osmotin under control of the constitutive promoter had a better resistance than those with osmotin under the control of the stress-inducible promoter. Evaluation in next generation was undertaken by studying bud break in transgenic and non-transgenic plants under simulated drought (2% polyethylene glycol) and salt stress (200 mM NaCl) conditions. The axillary buds of the selected transgenic lines had a better bud break percentage under stressed conditions than buds from non-transgenic mulberry lines. A biotic assay with Bombyx mori indicated that osmotin protein had no undesirable effect on silkworm rearing and feeding. We therefore conclude that 35S transgenic plants are better suited for both abiotic stress also biotic challenges (fungal), while the rd29A transgenic plants are more responsive to drought.

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Section: Osmotin Overexpression By the Camv 35s Promotermentioning

confidence: 91%

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

et al. 2010

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“…In the present studies we found that roots developed on charcoal medium without any plant growth regulators. This promotive effect of a growth regulator free medium on in vitro root induction has been recorded in other trees such as Robinia pseudoacacia (Kanwar et al 2009) and Morus alba (Agarwal and Kanwar 2007). Plant Cell Tiss Organ Cult (2010) 100:199-207 205 Acclimatization of in vitro raised plantlets…”

Section: Influence Of Agno 3 On Shoot Bud Regenerationmentioning

confidence: 92%

Comparison of in vitro regeneration pathways in Punica granatum L.

Kanwar

Joseph

Deepika

2009

Plant Cell Tiss Organ Cult

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When cotyledonary explants, excised from in vitro germinated seedlings, of pomegranate (Punica granatum L.) were incubated on solid Murashige and Skoog (1962) medium supplemented with 21 lM naptheleneacetic acid (NAA) and 9 lM 6-benzyladenine (BA), 80% of explants developed callus. A high frequency of shoot organogensis was obtained when explants were incubated on MS medium supplemented with 8 lM BA, 6 lM NAA, and 6 lM giberrellic acid (GA 3 ). However, adding 24 lM silver nitrate (AgNO 3 ) to this medium markedly enhanced shoot regeneration frequency (63%) and mean number of shoots per explant (11.26) and length of shoots (2.22 cm). Highest frequency of in vitro rooting, mean number of roots/shoot (4.32), and mean root length (2.71 cm) were obtained when regenerated shoots were transferred to halfstrength MS medium supplemented with 0.02% activated charcoal. Well-rooted plantlets were acclimatized, and then transferred to soil medium. Moreover, when zygotic embryos of P. granatum, excised from seeds collected at 16 weeks following full bloom, were incubated on MS medium containing 30 g l -1 sucrose, 15% coconut water, 21 lM NAA, and 9 lM BA, they developed the highest frequency of embryogenic callus, clumps with globular embryos, and mean number of both globular and heartshaped embryos per callus clump. Subjecting zygotic embryo explants to six-week dark incubation period was essential for embryogenic callus induction, and these were subsequently transferred to 16 h photoperiod for further growth and development of somatic embryos. Germination of somatic embryos was observed when these were transferred to MS medium was supplemented with 60 g l -1 sucrose.

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“…In addition, gynogenic haploids of M. alba were obtained by culturing unpollinated ovaries (Thomas et al 1999), triploid plants were regenerated from immature endosperm (Thomas et al 2000) and tetraploids were produced from apical buds following colchicine treatments (Chakraborti et al 1998). Somatic embryogenesis from mature zygotic embryos and the development of an efficient secondary embryogenic system have also been reported (Agarwal et al 2004;Agarwal and Kanwar 2007). On the other hand, black mulberry is a somewhat difficultto-propagate species, as it tends to bleed heavily when cut.…”

Section: Introductionmentioning

confidence: 98%

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

Ďurkovič

Kaňuchová

Kačík

et al. 2011

Plant Cell Tiss Organ Cult

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Proliferating shoot cultures of black mulberry (Morus nigra), derived from axillary buds of two donor trees designated as Mn1 and Mn2, more than 80 years of age, were established in vitro. Subsequently, shoot-tips were used to induce both axillary and adventitious shoot regeneration following incubation on Murashige and Skoog medium containing 14 different treatments of various concentrations of plant growth regulators, including 6-benzyladenine (BA), thidiazuron (TDZ), and combinations of BA with indole-3-butyric acid (IBA) and TDZ with BA. The highest shoot proliferation of 5.3 shoots per explant for the Mn1 tree and 6.9 shoots per explant for the Mn2 tree were obtained when explants were incubated on a medium containing 0.5 mg l -1 BA and 0.1 mg l -1 IBA. The maximum frequency of adventitious rooting was similar for both genotypes. Changes in lignin and cellulose content, macromolecular properties of dioxane and Klason lignins, lignin monomer composition, and macromolecular properties of cellulose were determined in 1-year-old and 3 year-old micropropagated plants, as well as in the donor trees. Lignin and cellulose properties were significantly dependent on the genotype, the age and the mutual interaction of both these factors. The syringyl to guaiacyl weight ratio in lignin rose with the age of the micropropagated plants. Moreover, the tensile strength of wood in 1-year-old plants was supported by a high cellulose degree of polymerization. The highest polydispersity index of cellulose was detected in 3-year-old plants.

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Comparison of genetic transformation in Morus alba L. via different regeneration systems

Cited by 35 publications

References 12 publications

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

Comparison of in vitro regeneration pathways in Punica granatum L.

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

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