Comparison of haemoglobin assessments by HemoCue and two automated haematology analysers in young Laotian children

Hinnouho, Guy‐Marino; Barffour, Maxwell A.; Wessells, K. Ryan; Brown, Kenneth H.; Kounnavong, Sengchanh; Chanhthavong, Bigphone; Ratsavong, Kethmany; Kewcharoenwong, Chidchamai; Hess, Sonja Y.

doi:10.1136/jclinpath-2017-204786

Cited by 49 publications

(55 citation statements)

References 34 publications

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“…3B) and from arterial, venous, and capillary blood among adult males and females aged 18 years and older. 18,84,95,113,[116][117][118][119]146 All comparisons reported higher Hb concentrations compared with the AHA (0.5−6.1 g/L), but were within the ±7% threshold for mean difference in Hb concentrations. 18,84,95,113,[116][117][118][119]146…”

Section: Comparability Of the Automated Hematology Analyzer With Hemomentioning

confidence: 99%

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Whitehead

Mei

Mapango

et al. 2019

Annals of the New York Academy of Sciences

123

100

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This paper describes and compares methods and analyzers used to measure hemoglobin (Hb) in clinical laboratories and field settings. We conducted a literature review for methods used to measure Hb in clinical laboratories and field settings. We described methods to measure Hb and factors influencing results. Automated hematology analyzer (AHA) was reference for all Hb comparisons using evaluation criteria of ±7% set by College of American Pathologists (CAP) and Clinical Laboratory Improvement Amendments (CLIA). Capillary fingerprick blood usually produces higher Hb concentrations compared with venous blood. Individual drops produced lower concentrations than pooled capillary blood. Compared with the AHA: (1) overall cyanmethemoglobin (1.0–8.0 g/L), WHO Colour Scale (0.5–10.0 g/L), paper-based devices (5.0–7.0 g/L), HemoCue® Hb-201 (1.0–16.0 g/L) and Hb-301 (0.5–6.0 g/L), and Masimo Pronto® (0.3–14.0 g/L) overestimated concentrations; (2) Masimo Radical® −7 both under- and overestimated concentrations (0.3–104.0 g/L); and (3) other methods underestimated concentrations (2.0–16.0 g/L). Most mean concentration comparisons varied less than ±7% of the reference. Hb measurements are influenced by several analytical factors. With few exceptions, mean concentration bias was within ±7%, suggesting acceptable performance. Appropriate, high-quality methods in all settings are necessary to ensure the accuracy of Hb measurements.This paper describes and compares methods and analyzers used to measure hemoglobin (Hb) in clinical laboratories and field settings. With few exceptions, mean concentration bias was within ±7%, suggesting acceptable performance. Appropriate, high-quality methods in all settings are necessary to ensure the accuracy of Hb measurements.

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Section: Comparability Of the Automated Hematology Analyzer With Hemomentioning

confidence: 99%

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Whitehead

Mei

Mapango

et al. 2019

Annals of the New York Academy of Sciences

123

100

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“…reported that Hemocue 301 system was less sensitive and detected lesser concentrations when compared with analyzer. 6 There was a significantly strong correlation between HemoCue Hb301 and automated analyzer (rvalue = 0.732, p <0.0001). However, the difference was small but clinically significant.…”

Section: Resultsmentioning

confidence: 87%

Comparison of haemoglobin assessment by HemoCue 301 and automated haematology analyser using flowcytometry among school going children: a one year study at a tertiary care hospital

Kumar

Ramalingam

2019

Int J Res Med Sci

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Background: Anaemia defined as reduction in the concentration in Haemoglobin is one of the key health indicators of health care system of the country. Accurate screening methods are required to estimate the levels of haemoglobin for diagnosing the cause of anaemia. Objectives of the study was to analyze and compare the results of haemoglobin concentrations estimated with automated haematology analyzer and point of care device HemoCue Hb301.Methods: It is a prospective cross-sectional study was conducted for one year after ethical approval. Non fasting capillary and venous blood samples were collected from the selected cases of children and Haemoglobin concentrations were estimated by automated analyzer and HemoCue Hb301 system and the values were noted. Quality control checks were performed for both. Statistical analysis was done using IBM SPSS Version 24.0.Results: Mean Hb% concentration was estimated in 108 children with 44 female and 64 males. The mean value of Automated hematology analyzer (11.965±1.012) was significantly higher when compared with the mean value of HemoCue Hb301 (11.697±1.312) (p=0.002). There was a significantly strong correlation between HemoCue Hb301and Automated hematology analyzer (r-value = 0.732, p <0.0001).Conclusions: The HemoCue is useful in many different settings and remains a widely used method in field settings as it has several advantages and is relatively inexpensive compared with automated haematology analysers. Further studies are needed to better understand potential sources of error in the Hb assessment by HemoCue with the aim to better train phlebotomists and implement appropriate standardised procedures.

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“…Yet, these findings are based on capillary blood analysis. In a recent study among children from Laos, Hb concentration by HemoCue was significantly higher in venous blood samples compared to capillary blood, resulting in different anemia prevalence data [52]. Although Hb measurement via HemoCue is thought to be more reliable in venous samples [53], some report that HemoCue showed poor agreement compared with automated hematology analyzers [52].…”

Section: Discussionmentioning

confidence: 99%

Impact of lunch provision on anthropometry, hemoglobin, and micronutrient status of female Cambodian garment workers: exploratory randomized controlled trial

et al. 2019

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Background: Lunch provision is expected to improve the nutritional status of Cambodian garment workers. The objective of this study is to evaluate the effects of a model lunch provision through a canteen on anthropometry, hemoglobin, and micronutrient status in female garment workers in Cambodia. Methods: This exploratory randomized controlled trial was implemented at a garment factory in Phnom Penh, Cambodia. Female workers (nulliparous, non-pregnant) were recruited and randomly allocated into an intervention arm (workday's lunch provision) and a control arm. Served lunch sets (~700 kcal on average) included diverse local dishes. Anthropometry (body mass index, weight, triceps skinfold thickness, and mid-upper arm muscle circumference), as well as hemoglobin, serum ferritin and soluble transferrin receptor, serum retinol binding protein, and serum folate concentrations were assessed at baseline and after 5 months of lunch provision. A general linear model with adjustments for baseline values was used to estimate intervention effects for each outcome variable. Results: Two hundred twenty-three women were recruited (n = 112 control and n = 111 intervention). 172 (n = 86 in each arm) completed the study. Baseline prevalence of underweight, anemia, depleted iron stores, and marginal iron stores, were 31, 24, 21, and 50%, respectively. Subjects were not affected by frank vitamin A or folate deficiency, whereas 30% showed a marginal folate status. Overall, mean changes in anthropometric variables, hemoglobin, and retinol binding protein were marginal and not significant among intervention subjects. Mean folate concentration increased insignificantly by + 1.1 ng/mL (− 0.02, 2.2) (p = 0.054). On the other hand, mean ferritin decreased by − 6.6 μg/L (− 11.9, − 1.3) (p = 0.015). Subgroup analysis prompts that effects are differently pronounced according to the baseline status of workers. Conclusions: Findings indicate that model lunch sets provided a beneficial amount of dietary folate, but need to be revisited for iron content and/or iron bioavailability. It is believed that distinct positive effects on anthropometry, hemoglobin, and micronutrient status can solely be expected in malnourished individuals. The authors suggest that similar larger trials, which include sets adapted to the concrete needs of workers affected by underweight, anemia and/or definite micronutrient deficiencies, should be performed.

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Comparison of haemoglobin assessments by HemoCue and two automated haematology analysers in young Laotian children

Cited by 49 publications

References 34 publications

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Comparison of haemoglobin assessment by HemoCue 301 and automated haematology analyser using flowcytometry among school going children: a one year study at a tertiary care hospital

Impact of lunch provision on anthropometry, hemoglobin, and micronutrient status of female Cambodian garment workers: exploratory randomized controlled trial

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