2004
DOI: 10.1002/elan.200402999
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Comparison of Hemoglobin and Myoglobin for In Situ Metabolite Generation in Chemical Toxicity Sensors Using a Metallopolymer Catalyst for DNA Damage Detection

Abstract: Detection of DNA damage can serve as the basis for rapid, inexpensive screening of the toxicity of new chemicals. Sensors for this purpose can be constructed on electrodes using layer-by-layer electrostatic adsorption of DNA and bioactivating proteins that mimic liver cytochrome P450s. This paper evaluates the use of the inexpensive protein hemoglobin (Hb) as the bioactivating ™enzyme∫ in such sensors. Stable films containing myoglobin (Mb) and DNA, and Hb and DNA, were assembled one layer at a time on rough p… Show more

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Cited by 17 publications
(11 citation statements)
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“…Components including ascorbate, uric acid, glutathione, and -carotene were rapidly oxidized by ferrylMb whereas ferrylHb oxidized the components very slowly (10,11). Hb and Mb promoted DNA oxidation similarly (12).…”
Section: Introductionmentioning
confidence: 99%
“…Components including ascorbate, uric acid, glutathione, and -carotene were rapidly oxidized by ferrylMb whereas ferrylHb oxidized the components very slowly (10,11). Hb and Mb promoted DNA oxidation similarly (12).…”
Section: Introductionmentioning
confidence: 99%
“…We also explore ART interaction with hemoglobin in solution using both chromatography and UV-vis techniques. We previously used hemoglobin and myoglobin films on electrode surface for bio-sensing and electro-catalysis 25,26 . In aqueous solution and in presence of Fe 2+ , ART was found to decompose 27,28 .…”
Section: Introductionmentioning
confidence: 99%
“…The consequent opening of the DNA double helix facilitated electrocatalytic oxidation of other guanine moieties mediated by the ruthenium complex. 118,119 Simultaneous optical and voltammetric detection of bioactivated genotoxicity was also reported employing ultrathin films of DNA, model metabolic enzymes (cytochrome P450 cam and myoglobin), and electrochemiluminescence (ECL) generating metallopolymer [Ru(bpy) 2 PVP 10 ] 21 on pyrolytic graphite (PG) electrodes. 120 Sensor film growth and component amounts were monitored using a quartz crystal microbalance (QCM).…”
Section: Layered Assemblies For Genotoxicity Screeningmentioning
confidence: 99%