Comparison of high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and ultra-high performance liquid chromatography with evaporative light scattering (UHPLC-ELSD) for the analyses of fructooligosaccharides in onion ( Allium cepa L.)

Pöhnl, Tobias; Böttcher, Christoph; Schulz, Hartwig; Stürtz, Melanie; Widder, Sabine; Carle, Reinhold; Schweiggert, Ralf M.

doi:10.1016/j.jfca.2017.08.001

Cited by 37 publications

(21 citation statements)

References 25 publications

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“…It is a well-recognized class of prebiotics [13–15], which are dietary ingredients that cannot be digested by human-produced digestive enzymes, yet they provide a health benefit to the host mediated by selectively stimulating the growth and/or activity of one or a limited number of host gut microbiota [16]. It is becoming increasingly clear that FOS can reduce or prevent gastroenteritis, inflammatory bowel disease (IBS), reduce the risk of colon cancer and reduce potentially pathogenic gastro-intestinal bacteria [17]. These activities are in line with the quality of Atractylodis rhizome for both natural and cultured products.…”

Section: Introductionmentioning

confidence: 99%

Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control

et al. 2019

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Background Atractylodis rhizoma, is the dried rhizomes of Atractylodes lancea (Thunb.) DC. or A. chinensis (DC.) Koidz. Both of two are pharmacologically and economically important, while with differences in efficacy. Therefore, an authentication system is vital for evaluation the quality and discrimination adulteration of Atractylodis rhizoma. Fructooligosaccharides (FOS), which are regarded as functional ingredients in Atractylodis rhizoma, have not been used for quality control of Atractylodis rhizoma for shortage of reference compounds. Results A HPLC-ELSD method was developed for the quantification of FOS in Atractylodis rhizoma. And chemometrics analysis showed that 2 markers including content of degree of polymerization (DP) 12 and total content of DP 3-15 could be used as the main distinctive elements for quality evaluation of Atractylodis rhizome. Actually, the separation and purification of high DP FOS, such as DP 12, is still a challenge because of high polarity. Then DP 5-based qualification evaluation was investigated for quality control of Atractylodis rhizoma. The results showed that A. lancea and A. chinensis could be clearly separated. Conclusions DP 5-based quantification method was credible and effectively adopted for solving the shortage of reference compounds and improving the quality control of Atractylodis rhizoma

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Section: Introductionmentioning

confidence: 99%

Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control

et al. 2019

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“…Nonstructural carbohydrates comprise the majority (up to 80%) of the DMC of onion bulbs . Major constituents are glucose, fructose, sucrose, inulin- and neo -inulin-type FOSs with a degree of polymerization (DP) in the range of 3–20. − DMC and soluble solid content (SSC) exhibit a strong linear relationship in onion and are positively correlated with the total concentration of FOS but negatively with the concentration of reducing sugars. − In addition, higher DMC is associated with a higher mean DP of FOSs . DMC displays a wide genetic variability in onion. , Regarding this trait, three types of onion cultivars are distinguished: fresh market types (5 to 9% DMC), storage types (8 to 12% DMC), and dehydrator types (>18% DMC) …”

Section: Introductionmentioning

confidence: 99%

Effect of Cultivar and Cultivation Year on the Metabolite Profile of Onion Bulbs (Allium cepa L.)

Böttcher

Krähmer

Stürtz

et al. 2018

J. Agric. Food Chem.

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This study investigated the variation of metabolite profiles of onion bulbs ( Allium cepa L.) depending on genetic and environmental factors. Nine onion cultivars ("Corrado", "Cupido", "Forum", "Hytech", "Picador", "Redlight", "Snowpack", "Stardust", "Sturon") with different scale color and dry matter content were grown in a two-year field trial. Using a recently established metabolite profiling approach based on liquid chromatography-coupled electrospray ionization quadrupole time-of-flight mass spectrometry, 106 polar and semipolar metabolites which belong to compound classes determining nutritional, sensory, and technological quality of onion bulbs such as saccharides, flavonoids, S-substitued cysteine conjugates, amino acids, and derived γ-glutamyl peptides were relatively quantitated in parallel. Statistical analyses of the obtained data indicated that depending on the compound class genetic and environmental factors differently contributed to variation of metabolite levels. For saccharides and flavonoids the genetic factor was the major source of variation, whereas for cysteine sulfoxides, amino acids, and peptides both genetic and environmental factors had a significant impact on corresponding metabolite levels.

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“…The summed area was quantified with a 6‐point standard curve (0–24 μg·ml −1 , linear, R 2 = 0.9988) of the summed peak areas in Orafti OPS inulin (β‐1,2‐linked fructan with a mean DP ≤10), which had a similar range of retention times (Figure 1b, bottom chromatogram). While the numerical values obtained may have been inaccurate due to the variability of response factors among short‐DP fructans, 21 differences between species and time points were captured by this method.…”

Section: Methodsmentioning

confidence: 99%

A chromatographic method to monitor fructan catabolism in two cool‐season grasses fermented by mixed bovine ruminal microbiota

2022

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BACKGROUND: Cool-season grasses contain fructans (fructose-based polymers), whose monomers are linked by β-2,6 or β-1,2 bonds. Fructans in the rumen are hydrolysed to fructose by bacterial enzymes, but little is known about the kinetics of fructan catabolism in the rumen. A chromatographic method, using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection, was developed to monitor changes in fructan profiles during the in vitro fermentation of timothy (Phleum pratense L.) and cocksfoot (Dactylis glomerata L.) by mixed rumen microbes. Sterile suspensions of freeze-dried and milled tissue in minimal media were inoculated with washed rumen microbial cells under anaerobic conditions, and aliquots of the incubations were profiled 0, 2, and 5 h post-inoculation.RESULTS: Timothy and cocksfoot differed in initial concentrations of glucose and sucrose, and in the amounts remaining 2 h post-inoculation (p < 0.01). The grasses also differed in initial concentrations of short-chain fructan, and in concentrations present 2 h post-inoculation (p = 0.01). Timothy and cocksfoot also differed in amounts of longchain fructans remaining 2 and 5 h post-inoculation (p = 0.03), and in the maximum degree of polymerisation after 2 h (p < 0.01).CONCLUSIONS: Over the first 2 h, long-chain fructans in cocksfoot decreased while short-chain fructans increased. In timothy, concentrations of short-chain fructans doubled, and the degree of polymerisation decreased by 15%. The results support a model of degradation of the longest fructan chains and conversion into shorter ones.

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Comparison of high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and ultra-high performance liquid chromatography with evaporative light scattering (UHPLC-ELSD) for the analyses of fructooligosaccharides in onion ( Allium cepa L.)

Cited by 37 publications

References 25 publications

Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control

Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control

Effect of Cultivar and Cultivation Year on the Metabolite Profile of Onion Bulbs (Allium cepa L.)

A chromatographic method to monitor fructan catabolism in two cool‐season grasses fermented by mixed bovine ruminal microbiota

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