Comparison of high-resolution gas chromatography and high-performance liquid chromatography for simultaneous determination of lamotrigine and carbamazepine in plasma

Abstract

Key WordsColumn liquid chromatography Gas ch romatog ra phy Lamotrigine Carbamazepine Human plasma SummaryRapid, simple ancl sensitive methods have been devised for simultaneous determination of Jamotrigine (LTG) and carbamazepine (CBZ) in microsamples of human plasma by high-performance liquid chromatography (HPLC) and high resolution gas chromatography (HRGC); both methods have been validated and compared.The HPLC method was more sensitive than HRGC for both drugs, LTG and CBZ. The lowest quantitation limits (LOQ) of plasma LTG concentrations were 0.1 #g.mL ] (HPLC) and 0.5 #g.mL ] (HRGC); forCBZ theywere 0.1 #g.mL ] (HPLC) and 0.25 #g.mL ] (HRGC).Both methods, are sensitive enough to work with microsamples at subtherapeutic, therapeutic and toxic concentrations, being reliable and practical for routine therapeutic monitoring of LTG. The HPLC method proposed is suitable also for pharmacokinetic studies or chilcl patient, routine therapeutic-drug monitoring where there is a need for sensitive methods and small sample sizes.

Antipsychotic and Antiepileptic Drugs in Bipolar Disorder: The Importance of Therapeutic Drug Monitoring

“…Few studies can be found in the literature regarding the determination of LMT as a single analyte or together with other drugs or its metabolites in biological fluids by means of HPLC with UV (or diode array) [107,[185][186][187][188][189][190][191][192][193][194][195], or mass spectrometry [196][197][198] detection and by means of gaschromatography (GC) [199][200][201] or capillary electrophoresis (CE) methods [202][203][204][205]. Only a few papers describe the determination of LMT and its metabolites in biological fluids by means of HPLC with DAD detection [195], electrokinetic chromatography (MEKC) [205], Automated Sequential Trace Enrichment of Dialysates HPLC [206] and HPLC mass spectrometry [196].…”

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

“…A literature review revealed that different methods have been reported for the determination of CBZ. These include spectrophotometry , photoinduced fluorimetry , voltammetry , high‐performance liquid chromatography , spectrofluorimetry , micellar electrokinetic capillary chromatography , solid‐phase extraction , chemiluminescence , liquid chromatography , ion chromatography and gas chromatographic methods .…”

High-performance thin-layer chromatographic determination of lamotrigine in serum

“…These include HPLC [4][5][6][7][8][9], GC [10][11][12], GC-MS [13,14], radioimmunoassay [15], electrospray ionization-mass spectrometry (ESI-MS) [16], capillary electrophoresis [17][18][19], etc. Unlike HPTLC, all reported methods are cumbersome, time consuming and costlier techniques for estimation of drug in biological samples.…”