High-performance thin-layer chromatographic determination of lamotrigine in serum

Patil, Kuldeep M.; Bodhankar, S.L.

doi:10.1016/j.jchromb.2005.06.029

Cited by 28 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These AEDs were selected based on a recent audit by our group of the most commonly prescribed AEDs in children with epilepsy in N. Ireland (data not published). Determination of the AEDs of interest in plasma or serum samples using HPLC with UV or diode array detection (DAD) detection has been reported in the literature [29][30][31][32][33]. The analytical methodology was optimised and applied in the analysis of the AEDs in DBS samples of children with epilepsy collected at the clinic and in the patients' home for adherence determination.…”

Section: Introductionmentioning

confidence: 99%

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Shah

Hawwa

Millership

et al. 2013

Journal of Chromatography B

View full text Add to dashboard Cite

Publisher rights This is the author's version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, [VOL 923-924, (2013)] General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The authors wish also to acknowledge the Ministry of Higher Education, Malaysia for the studentship provided. The funder of the current study had no involvement in: (1) design and conduct of the study; (2) collection, management, analysis, and interpretation of the data;and (3) preparation, review, or approval of the manuscript. Conflict of Interest:The authors have no conflicts of interest relevant to this article to disclose.Page 2 Chromatographic separation of the AEDs was achieved using Waters XBridge™ C18 column with a gradient system. The developed method was linear over the concentration ranges studied with r ≥0.995 for all compounds. The lower limits of quantification (LLOQs) were 2, 1, 2, 0.5 and 1 μg/mL for LVT, LTG, PHB, CBZE and CBZ, respectively. Accuracy (%RE) and precision (%CV) values for within and between day were <20% at the LLOQs and <15% at all other concentrations tested. This method was successfully applied to the analysis of the AEDs in DBS samples taken from children with epilepsy for the assessment of their adherence to prescribed treatments. Page 3 of 35A c c e p t e d M a n u s c r i p t 4 Highlights: We report a simple method for the analysis of four antiepileptic drugs in DBS samples  The method was applied to DBS samples collected from children with epilepsy  Such technique has potential in assessing adherence to AEDs using home sampling

show abstract

Section: Introductionmentioning

confidence: 99%

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Shah

Hawwa

Millership

et al. 2013

Journal of Chromatography B

View full text Add to dashboard Cite

show abstract

“…In recent years HPTLC has gained importance since it allows reliable quantification of analytes at micro and even nanogram levels 9 . Lamotrigine, an antiepileptic drug in human serum has been estimated by HPTLC method 10 . HPTLC was also developed to study the Dog serum concentration of Pentoxifylline 11 .…”

Section: Introductionmentioning

confidence: 99%

Determination of Andrographolide in Andrographis Paniculata Extracts With and Without Human Serum by High Performance Thin Layer Chromatography

Mohan¹

2014

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Andrographis paniculata (Acanthaceae)commonly known as Kalmegh is used both in Ayurvedic and Unani system of medicines for a number of ailments. Kalmegh contains bitter principles-Andrographolide, 14-deoxy-11, 12-didehydroandrographolide, neoandrographolide, 14-deoxy-11, oxyandrographolide, 14-deoxy-andrographolide, and flavonoids. HPTLC analysis indicates that the maximum amount of andrographolide was present in extracts of Andrographis paniculata prepared by refluxation. Amount of Andrographolide in all extracts with and without serum along with the % recovery is compared and the variation was found to be < 15 %. The HPTLC method developed for estimation of andrographolide is simple, sensitive and accurate.

show abstract

“…Chromatographic techniques have been widely employed for the determination of LMT in body fluids. Published methods for the determination of LMT in biological samples include high-performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10], high-performance thin layer chromatography (HPTLC) [11] and gas-chromatography (GC) [12] and for assay in pharmaceuticals include planar chromatography [13], TLC and HPLC [14], HPLC and GC [15] and capillary electrophoresis [16,17] have been reported. Two immunoassay techniques [18,19] have also been developed for determination of LMT in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Simple and Sensitive Spectrophotmetric Determination of Lamotrigine in Pure Form and in Dosage Forms

Rajendraprasad¹,

Basavaiah²

2012

Pharm Anal Acta

View full text Add to dashboard Cite

Two new simple, sensitive and cost-effective spectrophotometric methods for the determination of lamotrigine (LMT) in bulk drug and in tablets are described. The methods are based on the measurement of absorbance of LMT either in 0.1 M H 2 SO 4 (method A) or in methanol (method B) at 225 nm. Linearity was found to be in the ranges, 0.5-5.0 and 1.25-12.5 µgmL-1 LMT, for method A and method B, respectively with apparent molar absorptivity values of 8.65×10 4 and 2.11×10 4 l mol-1 cm-1. The Sandell sensitivity values, limits of detection (LOD) and quantification (LOQ) values have also been reported for both the methods. The accuracy and precision of the methods were evaluated on intra-day and inter-day basis; the relative error (%RE) and the relative standard deviation (RSD) were <2.0%. The proposed methods were applied to the determination of the examined drug in coated tablet and no interference from any common pharmaceutical additives and diluents was observed. Results of assay were validated statistically by parallel analysis and by recovery studies.

show abstract

High-performance thin-layer chromatographic determination of lamotrigine in serum

Cited by 28 publications

References 19 publications

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Determination of Andrographolide in Andrographis Paniculata Extracts With and Without Human Serum by High Performance Thin Layer Chromatography

Simple and Sensitive Spectrophotmetric Determination of Lamotrigine in Pure Form and in Dosage Forms

Contact Info

Product

Resources

About