Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum

Chen, L.J.; Song, Hang; Lan, Xianqiu; Games, David E.; Sutherland, Ian

doi:10.1016/j.chroma.2004.11.072

Cited by 55 publications

(29 citation statements)

References 8 publications

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“…Compared with traditional separation methods, such as silica gel column chromatography, thin-layer chromatography (TLC), HSCCC has a series of advantages including high sample loading capacity, high sample recovery, low solvent consuming and no irreversible absorption. It is especially suitable for separation and purification of active components from natural products [9][10][11][12][13][14]. According to our literature research, up to now, there is no report on the use of HSCCC for the separation and purification of daurisolin, dauricine, daurinoline and dauricicoline from the roots of M. dauricum.…”

Section: Introductionmentioning

confidence: 99%

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Luo

Peng

et al. 2010

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Luo

Peng

et al. 2010

Journal of Chromatography B

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“…Countercurrent chromatography (CCC) was first invented by Ito et al in the early 1970s, which provides an advantage over the conventional separation techniques by not using a solid stationary phase and eliminating the irreversible adsorption of the samples from the support. In the intervening years, the method has been radically improved in terms of resolution, separation time, and sample loading capacity by the development of high speed countercurrent chromatography (HSCCC), which yields a highly efficient separation and has been widely used for isolation and purification of alkaloids, [1,2] flavonoids, [3,4] quinones, [5,6] coumarins, [7] lignans, [8] and other natural products [9] from traditional Chinese medicines (TCMs).…”

Section: Introductionmentioning

confidence: 99%

New Approach for Application of High Speed Countercurrent Chromatography Coupled with Direct Injection of the Powders of a Raw Material without any Preparation, for Isolation and Separation of Four Alkaloids with High Recoveries from Coptis chinensis Franch

Peng

Han

et al. 2007

Journal of Liquid Chromatography & Related Technologies

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Generally, before a successful high speed countercurrent chromatography (HSCCC) separation, crude samples were extracted from medicinal plants by aqueous ethanol or other organic solvents, and then the extracts were cleaned up by liquid-liquid extraction or column chromatography to produce the suitable samples for further purification, which were hostile for our environment and natural resource reserve because more time and solvent were consumed, and there was sample loss with a low recovery. In view of these drawbacks, a new approach of using HSCCC coupled with direct injection of the powders of the raw material without any preparation method, was successfully developed to separate and purify jatrorrhizine, coptisine, palmatine, and berberine from Coptis chinensis Franch, with high recoveries of over 92% determined by high performance liquid chromatography and standards compared with approximately 70% recoveries in common HSCCC purification. Our study showed a new approach in the HSCCC research field for combining the extraction and separation process together in the HSCCC column only, to eliminate sample loss and which decreases solvent and length of time.

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“…In the previous studies, the analytical CCC had been used to support method development and solvent selection in preparative HSCCC with its short separation time and minimum solvent consumption. [3,8] The results clearly demonstrated that it is feasible to evaluate raw herb's quality by this method.…”

Section: Resultsmentioning

confidence: 85%

Rapid and Simple Method for Quality Control of Raw Materials of Herbs by HSCCC

Wang

Liu

Zhang

et al. 2007

Journal of Liquid Chromatography & Related Technologies

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The component of the traditional Chinese medicine (TCM) can be influenced by soils, climates, and growth stages. The quality of TCM mostly depends on the quality of the raw materials of the used herbs. A portable instrument and simple analysis method are urgently needed to be used for quality control of the raw herbs. In this study, analytical CCC (Model HS06) was applied to analyze three herbs including Fructus Arctii (Arctium lappa L.), Cortex Magnoliae Officinalis (Magnolia officinalis Rehd. et Wils.), and Fructus Psoraleae (Psoralea corylitolia L.), which were collected from different growth locations. The results showed that analytical CCC gave similar resolution as preparative CCC. In this analytical CCC operation, the crude sample is loaded directly without any pre-treatment, and only 1-10 mg sample is used for each injection. The separation time is often shorter than 1 hour and can compete with that of HPLC. HSCCC chromatograms can be used as the comparison patterns for the content control of the major bioactive compounds. The results demonstrated that it is feasible to control the raw herb's quality by analytical CCC.

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Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum

Cited by 55 publications

References 8 publications

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

New Approach for Application of High Speed Countercurrent Chromatography Coupled with Direct Injection of the Powders of a Raw Material without any Preparation, for Isolation and Separation of Four Alkaloids with High Recoveries from Coptis chinensis Franch

Rapid and Simple Method for Quality Control of Raw Materials of Herbs by HSCCC

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