Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs

Varello, Katia; Pezzolato, Marzia; Mascarino, Daniela; Ingravalle, Francesco; Caramelli, Maria; Bozzetta, Elena

doi:10.1177/104063870802000204

Cited by 36 publications

(34 citation statements)

References 29 publications

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“…There was good agreement between the histopathology findings, bacterial culture and identification (Rivera-Posada et al 2011a,b, present study), suggesting that histopathological examination is a reliable tool that allowed identification of typical vibriosis lesions and could contribute to the differentiation from other causes. Varello et al (2008) compared histologic techniques and bacteria cultures for the diagnosis of bovine tuberculosis reporting similar results.…”

Section: Discussionsupporting

confidence: 49%

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Rivera-Posada¹,

Pratchett²,

Owens³

2011

Dis. Aquat. Org.

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ABSTRACT:We assessed histological changes in the tissues of the crown-of-thorns starfish Acanthaster planci (COTS) after injection of thiosulfate-citrate-bile-sucrose agar (TCBS) which was used as a disease inducer (potential outbreak control method), by conventional and scanning electron microscopy. Digestive glands were processed and stained with hematoxylin and eosin to describe the histological architecture of the intestinal epithelium. Subsequently comparison of healthy versus infected tissues and Gram stains were carried out to confirm bacterial occurrence on infected tissues, characterize the structural changes induced by bacterial communities in COTS tissues, and to determine if the histopathological changes of intestinal tissues were consistent with vibrio infection. TCBS injections induced marked epithelial desquamation, hypertrophy and hypersecretion of glandular cells, epithelial cell destruction, pyknosis, reduction of thickness and disorganization of connective tissue and associated nerve plexus, presence of bacterial colonies, irregular eosinophilic foci in glandular cells, brush border disruption, atrophy and detachment of intestinal microvilli and cell debris in the lumen. All these changes were attributed to a fulminating systemic dysbiosis and were consistent with vibrio infections.

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Section: Discussionsupporting

confidence: 49%

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Rivera-Posada¹,

Pratchett²,

Owens³

2011

Dis. Aquat. Org.

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“…VARELLO et al (2008) analyzed 173 suspected tissue samples from bovines and identified 117 of them (67.63%) as tuberculosis using Hematoxylin-eosin staining, but when Ziehl-Neelsen staining was used, AFB was identified in only 31 samples (17.91%). This data underlines the need to associate histopathological characterization and AFB identification, since the latter can be limited due to low quantity of bacteria in lesions.…”

Section: Discussionmentioning

confidence: 99%

Comparison between tests for tuberculosis diagnosis in slaughtered bovines

et al. 2018

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RESUMO:O objetivo deste estudo foi a comparação entre diferentes testes de diagnóstico para tuberculose bovina. Foram realizados o isolamento bacteriano, a caracterização histopatológica, a identificação de bacilos álcool-ácido resistentes e a detecção do DNA de M. bovis pela reação em cadeia da polimerase, em bovinos adultos abatidos em matadouros frigoríficos sob o Serviço de Inspeção Federal, valendo-se de amostras de linfonodos com lesões macroscópicas sugestivas de tuberculose, identificadas e coletadas durante o abate. O isolamento bacteriano foi realizado pelo cultivo em meios de cultura sólidos; a caracterização histopatológica, pela coloração com hematoxilina-eosina; e a identificação de bacilos álcool-ácido resistentes foi feita pela coloração de Ziehl-Neelsen. A detecção de DNA foi realizada em amostra extraída das lesões sugestivas de tuberculose pela reação em cadeia da polimerase, seguida da nested reação em cadeia da polimerase e por meio das colônias isoladas para identificação do M. bovis, utilizando-se também da reação em cadeia da polimerase . Os resultados obtidos permitiram concluir que os testes histopatológicos, o isolamento bacteriano e a identificação de bacilos álcool-ácido resistentes são aconselháveis para o diagnóstico da tuberculose bovina. Além disso, ensaios de reação em cadeia da polimerase utilizando amostras de lesões sugestivas de tuberculose são um modo mais rápido e promissor para diagnosticar a enfermidade, no entanto não deve ser utilizado sozinho, em virtude da baixa sensibilidade apresentada neste estudo. PALAVRAS-CHAVE: doenças infecciosas; Mycobacterium bovis;tuberculose; zoonose. ABSTRACT:Our goal for this article is to compare several different diagnosis tests for bovine tuberculosis identification. We have performed bacterial isolation, histopathological characterization, acid-fast bacilli (AFB) identification and M. bovis DNA detection. Lesions suggestive of Tuberculosis were sampled from bovine lymph nodes during slaughtering of bovines at an abattoir that operates under federal inspection. The bacterial isolation was performed in solid culture mediums, the histopathological characterization was made by Hematoxylin-eosinstaining, and AFB identification by Ziehl-Neelsen staining. Bacterial DNA detection was performed by Polymerase Chain Reaction (PCR) using DNA from two different sources, directly collected from the tuberculosis-like lesions (PCR followed by nested PCR) and from isolated bacteria. We have concluded that the multi-step approach, including histopathological characterization, bacterial isolation and AFB identification, is strongly recommended to diagnose tuberculosis in bovines. Furthermore, PCR assays using specimens of lesions suggestive of tuberculosis are a faster and more promising way to diagnose the disease. However, it should not be used alone due to the low sensitivity shown in this study.

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“…An estimate of granuloma area in a lung section was determined by taking the average of approximately 5 microscopic (x100) ϐiel-ds examined in one plane of section. Despite been lung samples instead lymph nodes, samples were categorized according to Varello et al (2008), as follows: Positive: tubercular granuloma displaying central necrosis with or without mineralization surrounded by macrophages, lymphocytes, plasma cells, neutrophils, epithelioid cells, and Langerhan's giant cells, and enclosed partly or completely by a thin capsule. Inconclusive: lesion characterized by irregular with no capsulated clusters of epithelioid macrophages; associated with a not Langerhan's-type multinucleated giant cells and necrosis.…”

Section: Methodsmentioning

confidence: 99%

Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd

et al. 2012

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The standard method for detection of bovine tuberculosis (TB) is the single intradermal tuberculin test (SITT). Nevertheless, current studies suggest that a single test is not enough to detect all cattle infected by TB, particularly when animals present different stages of infection. A dairy herd comprised of 270 cows was studied and 15 were reactive to SITT plus nine inconclusive animals. Blood samples (for IFN and ELISA) were collected from these 24 cows. At 30 days after injection of PPD, all the cows that were reactive to any of the employed tests were slaughtered, and tissues were processed by Bacteriology, Histopathology (HP) and PCR. According to HP 33.4% of the animals were positive, 45.8% inconclusive and 20.8% were negative. The inconclusive samples came from IFN positive animals, signalizing recent infection. Regarding the animals that were negative to HP, all of them were identiϐied by IFN while ELISA was negative. Immune responses are different in recent and advanced infections, what supports the identiϐication between chronically or recently infected animals. This multidisciplinary approach is mandatory for the interpretation of the various tools that are frequently employed for the diagnosis of TB and mainly to identify all infected animals.INDEX TERMS: Bovine tuberculosis, diagnosis, multidisciplinary approach, histopathology.

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Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs

Cited by 36 publications

References 29 publications

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). II. Histopathological changes

Comparison between tests for tuberculosis diagnosis in slaughtered bovines

Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd

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